scholarly journals Global gene expression reveals an increase of HMGB1 and APEX1 proteins and their involvement in oxidative stress, apoptosis and inflammation pathways among beta‐thalassaemia intermedia and major phenotypes

2019 ◽  
Vol 186 (4) ◽  
pp. 608-619 ◽  
Author(s):  
João Pedro Maia de Oliveira da Silva ◽  
Ana Flávia Brugnerotto ◽  
Karen S. Romanello ◽  
Karina K. L. Teixeira ◽  
Carolina Lanaro ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Global Gene Expression ◽  
Beta Thalassaemia ◽  
Apoptosis And Inflammation ◽  
Thalassaemia Intermedia

scholarly journals Transcriptome Sequencing Data of Bacillus anthracis Vollum ΔhtrA and Its Parental Strain, Isolated under Oxidative Stress

2020 ◽  
Vol 9 (35) ◽  
Author(s):  
Theodor Chitlaru ◽  
Inbar Cohen-Gihon ◽  
Ofir Israeli ◽  
Uri Elia ◽  
Galia Zaide ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Bacillus Anthracis ◽  
Transcriptome Sequencing ◽  
Global Gene Expression ◽  
Data Sets ◽  
Transcriptome Data ◽  
Wild Type ◽  
Sequencing Data ◽  
Content Type

ABSTRACT The high-temperature requirement chaperone/protease (HtrA) is involved in the stress response of the anthrax-causing pathogen Bacillus anthracis. Resilience to oxidative stress is essential for the manifestation of B. anthracis pathogenicity. Here, we announce transcriptome data sets detailing global gene expression in B. anthracis wild-type and htrA-disrupted strains following H2O2-induced oxidative stress.

scholarly journals Stat3 oxidation-dependent regulation of gene expression impacts on developmental processes and involves cooperation with Hif-1α

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0244255
Author(s):  
Michela Grillo ◽  
Carolyn Palmer ◽  
Nadine Holmes ◽  
Fei Sang ◽  
Andrew C. Larner ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Cell Metabolism ◽  
Second Messengers ◽  
Regulation Of Gene Expression ◽  
Global Gene Expression ◽  
Gene Promoters ◽  
Immune Functions ◽  
Developmental Processes ◽  
The Impact

Reactive oxygen species are bona fide intracellular second messengers that influence cell metabolism and aging by mechanisms that are incompletely resolved. Mitochondria generate superoxide that is dis-mutated to hydrogen peroxide, which in turn oxidises cysteine-based enzymes such as phosphatases, peroxiredoxins and redox-sensitive transcription factors to modulate their activity. Signal Transducer and Activator of Transcription 3 (Stat3) has been shown to participate in an oxidative relay with peroxiredoxin II but the impact of Stat3 oxidation on target gene expression and its biological consequences remain to be established. Thus, we created murine embryonic fibroblasts (MEFs) that express either WT-Stat3 or a redox-insensitive mutant of Stat3 (Stat3-C3S). The Stat3-C3S cells differed from WT-Stat3 cells in morphology, proliferation and resistance to oxidative stress; in response to cytokine stimulation, they displayed elevated Stat3 tyrosine phosphorylation and Socs3 expression, implying that Stat3-C3S is insensitive to oxidative inhibition. Comparative analysis of global gene expression in WT-Stat3 and Stat3-C3S cells revealed differential expression (DE) of genes both under basal conditions and during oxidative stress. Using differential gene regulation pattern analysis, we identified 199 genes clustered into 10 distinct patterns that were selectively responsive to Stat3 oxidation. GO term analysis identified down-regulated genes to be enriched for tissue/organ development and morphogenesis and up-regulated genes to be enriched for cell-cell adhesion, immune responses and transport related processes. Although most DE gene promoters contain consensus Stat3 inducible elements (SIEs), our chromatin immunoprecipitation (ChIP) and ChIP-seq analyses did not detect Stat3 binding at these sites in control or oxidant-stimulated cells, suggesting that oxidised Stat3 regulates these genes indirectly. Our further computational analysis revealed enrichment of hypoxia response elements (HREs) within DE gene promoters, implying a role for Hif-1. Experimental validation revealed that efficient stabilisation of Hif-1α in response to oxidative stress or hypoxia required an oxidation-competent Stat3 and that depletion of Hif-1α suppressed the inducible expression of Kcnb1, a representative DE gene. Our data suggest that Stat3 and Hif-1α cooperate to regulate genes involved in immune functions and developmental processes in response to oxidative stress.

Vitamin E Supplementation Reduces Oxidative Stress in Beta Thalassaemia Intermedia

2008 ◽  
Vol 120 (4) ◽  
pp. 225-231 ◽  
Author(s):  
W.P. Pfeifer ◽  
G.R. Degasperi ◽  
M.T. Almeida ◽  
A.E. Vercesi ◽  
F.F. Costa ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Beta Thalassaemia ◽  
Thalassaemia Intermedia ◽  
Vitamin E Supplementation

scholarly journals Forty-eight hours of unloading and 24 h of reloading lead to changes in global gene expression patterns related to ubiquitination and oxidative stress in humans

2010 ◽  
Vol 109 (5) ◽  
pp. 1404-1415 ◽  
Author(s):  
Kimberly A. Reich ◽  
Yi-Wen Chen ◽  
Paul D. Thompson ◽  
Eric P. Hoffman ◽  
Priscilla M. Clarkson
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Stress Response ◽  
Expression Patterns ◽  
Global Gene Expression ◽  
Canonical Pathway ◽  
Gene Expression Patterns ◽  
Heme Oxygenase 1 ◽  
Short Term ◽  
Qrt Pcr

Although short-term disuse does not result in measurable muscle atrophy, studies suggest that molecular changes associated with protein degradation may be initiated within days of the onset of a disuse stimulus. We examined the global gene expression patterns in sedentary men ( n = 7, mean age ± SD = 22.1 ± 3.7 yr) following 48 h unloading (UL) via unilateral lower limb suspension and 24 h reloading (RL). Biopsy samples of the left vastus lateralis muscle were collected at baseline, 48 h UL, and 24 h RL. Expression changes were measured by microarray and gene clustering; identification of enriched functions and canonical pathways were performed using the Database for Annotation, Visualization and Integrated Discovery (DAVID) and Ingenuity Pathway Analysis (IPA). Four genes were validated with quantitative RT-PCR (qRT-PCR), and protein levels were measured with Western blot. Of the upregulated genes after UL, the most enriched functional group and highest ranked canonical pathway were related to protein ubiquitination. The oxidative stress response pathway was the second highest ranked canonical pathway. Of the downregulated genes, functions related to mitochondrial metabolism were the most highly enriched. In general, gene expression patterns following UL persisted following RL. qRT-PCR confirmed increases in mRNA for ubiquitin proteasome pathway-related E3 ligase Atrogin1 (but not accompanying increases in protein products) and stress response gene heme oxygenase-1 (HMOX, which showed a trend toward increases in protein products at 48 h UL) as well as extracellular matrix (ECM) component COL4A3. The gene expression patterns were not reversed on RL, suggesting that molecular responses to short-term periods of skeletal muscle inactivity may persist after activity resumes.

scholarly journals Genistein Induces Deleterious Effects during Its Acute Exposure in Swiss Mice

2014 ◽  
Vol 2014 ◽  
pp. 1-14 ◽  
Author(s):  
Prabhat Singh ◽  
Sharad Sharma ◽  
Srikanta Kumar Rath
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Differentially Expressed Genes ◽  
Global Gene Expression ◽  
Differentially Expressed ◽  
Therapeutic Effects ◽  
Biologically Relevant ◽  
Swiss Mice ◽  
Tissue Homogenates ◽  
Cellular Pathways

Genistein is a soy derived isoflavone. It has wide variety of therapeutic effects against certain diseases including cancer. Although toxic effects of genistein have been studied, its effect on the gene expression and the reason behind toxicity have not been identified yet. In the present study, genistein was administered to age and body weight matched Swiss mice at the doses of 125, 250, 500 and 1000 mg/kg. The biomarkers of hepatotoxicity in serum, liver histology, oxidative stress parameters in tissue homogenates, and global gene expression were examined. Elevated alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) levels and degenerated liver tissue were observed in 500, and 1000 mg/kg genistein treated groups. Oxidative stress was significant at these doses as considerable increase in lipid peroxidation (LPO) and decrease in total glutathione (GSH) were observed. Gene expression analysis showed 40 differentially expressed genes at twofold change andP<0.05.Differentially expressed genes were corresponding to different biologically relevant pathways including metabolic and oxidative stress pathways. In 500 mg/kg group, Cyp4a14, Sult1e1, Gadd45g, Cidec, Mycs, and so forth genes were upregulated. These results suggested that the higher dose of genistein can produce several undesirable effects by affecting multiple cellular pathways.

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