Basket‐weave structure in the stratum corneum is an important factor for maintaining the physiological properties of human skin as studied using reconstructed human epidermis and tape stripping of human cheek skin

2019 ◽  
Vol 182 (2) ◽  
pp. 364-372 ◽  
Author(s):  
H. Goto ◽  
A. Tada ◽  
A. Ibe ◽  
Y. Kitajima
Keyword(s):  
Stratum Corneum ◽  
Human Skin ◽  
Human Epidermis ◽  
Tape Stripping ◽  
Reconstructed Human Epidermis ◽  
Weave Structure ◽  
Physiological Properties

scholarly journals Tape stripping method is useful for the quantification of antimicrobial peptides on the human skin surface including the stratum corneum

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Shigeyuki Ono ◽  
Nobuhiko Eda ◽  
Takuya Mori ◽  
Atsuko Otsuka ◽  
Nobuhiro Nakamura ◽  
...  
Keyword(s):  
Antimicrobial Peptides ◽  
Stratum Corneum ◽  
Human Skin ◽  
Significant Positive Correlation ◽  
Skin Surface ◽  
Tape Stripping ◽  
Established Method ◽  
Positive Correlation ◽  
Physiological Importance ◽  
Stripping Method

Abstract Antimicrobial peptides (AMPs) play an important role in innate immunity in human skin. It is known that AMPs mainly function in the stratum corneum. Therefore, AMP concentrations in the stratum corneum need to be precisely measured to clarify functional and physiological importance of AMPs in cutaneous defence. Tape stripping (TS) is a well-established method by which components in the stratum corneum can be collected. However, the usefulness of the TS method for measuring AMP concentration in human skin remains unclear. Therefore, we compared it with another popular method, skin rinsing, which had been established as a method for measuring AMP concentration in human skin. When investigated on healthy medial forearm using RNase 7, which is one of the typical AMPs, as an index, there was a significant positive correlation between RNase 7 concentrations measured by the TS method at adjacent forearm sites, demonstrating the reproducibility of the TS method. Next, a significant positive correlation was detected in RNase 7 concentrations measured using the TS and the skin rinsing method, indicating that the TS method is comparable to the skin rinsing method. Thus, we speculate that the TS method is useful for measuring AMP concentration in human skin.

Permeability of the reconstructed human epidermis model Episkin® in comparison to various human skin preparations☆

2007 ◽  
Vol 66 (1) ◽  
pp. 127-134 ◽  
Author(s):  
Frank Netzlaff ◽  
Monika Kaca ◽  
Udo Bock ◽  
Eleonore Haltner-Ukomadu ◽  
Peter Meiers ◽  
...  
Keyword(s):  
Human Skin ◽  
Human Epidermis ◽  
Reconstructed Human Epidermis

scholarly journals The Reconstructed Human Epidermis in vitro — a Model for Basic and Applied Research of Human Skin

2020 ◽  
Vol 96 (2) ◽  
pp. 24-34
Author(s):  
Arkadii K. Beilin ◽  
Alexandra L. Rippa ◽  
Valentin I. Sharobaro ◽  
Nadejda G. Gurskaya ◽  
Ekaterina A. Vorotelyak
Keyword(s):  
Basement Membrane ◽  
Stratum Corneum ◽  
Basal Layer ◽  
Human Epidermis ◽  
Human Donor ◽  
Laminin 5 ◽  
Reconstructed Human Epidermis ◽  
Donor Skin ◽  
Granular Layers

Background. The reconstructed human epidermis (RE) is an in vitro tissue-engineering construct similar to the native epidermis. Objective. To develop a full-layer RE. Describe its structure: determine the presence of all layers of the epidermal component, including basal, spinous and granular layers and stratum corneum of the epidermis; detect the basement membrane, the border between the epidermal and mesenchymal component. Materials and methods. Isolation of keratinocytes and fibroblasts from human donor skin. Cultivation of keratinocytes and fibroblasts in vitro under 2D conditions, cell subculturing and 3D modeling of RE, obtaining cryosections, histological staining, immunohistochemical (IHC) study with antibodies to cytokeratins 14 and 10, Ki67 protein, loricrin, laminin 5 and plectin. Results. A technique was developed for the formation of RE. Histological examination showed that the stratification of keratinocyte layers occurs during the formation of RE. Layers are formed including basal, spinous and granular layers and stratum corneum. The IHC study has shown the proliferative activity of keratinocytes of the basal layer and has detected the presence of marker proteins of keratinocytes at different stages of differentiation. RE basal keratinocytes, like native ones, form hemidesmosomes and synthesize basement membrane proteins. Conclusions. A full-layer human RE was obtained in vitro. RE meets all the characteristics of the native epidermis and it is suitable for basic and practical research in the field of skin biology, dermatology, and cosmetology.

Effect of Acetone and Kerosene on Skin Ultrastructure

1972 ◽  
Vol 30 ◽  
pp. 92-93
Author(s):  
A. P. Lupulescu ◽  
H. Pinkus ◽  
D. J. Birmingham
Keyword(s):  
Electron Microscopy ◽  
Human Skin ◽  
Osmium Tetroxide ◽  
Human Epidermis ◽  
Ultrastructural Changes ◽  
Chemical Agents ◽  
Skin Ultrastructure ◽  
Volar Surface

Our laboratory is engaged in the study of the effect of different chemical agents on human skin, using electron microscopy. Previous investigations revealed that topical use of a strong alkali (NaOH 1N) or acid (HCl 1N), induces ultrastructural changes in the upper layers of human epidermis. In the current experiments, acetone and kerosene, which are primarily lipid solvents, were topically used on the volar surface of the forearm of Caucasian and Negro volunteers. Skin specimens were bioptically removed after 90 min. exposure and 72. hours later, fixed in 3% buffered glutaraldehyde, postfixed in 1% phosphate osmium tetroxide, then flat embedded in Epon.

Changes in corneocyte element concentrations occur in skin inner stratum corneum

1990 ◽  
Vol 48 (2) ◽  
pp. 146-147
Author(s):  
R. R. Warner
Keyword(s):  
Stratum Corneum ◽  
Human Skin ◽  
Element Concentration ◽  
Skin Barrier ◽  
Barrier Properties ◽  
Brick Wall ◽  
Inorganic Elements ◽  
Dry Skin ◽  
Skin Biopsies ◽  
Intercellular Lipid

Keratinocytes undergo maturation during their transit through the viable layers of skin, and then abruptly transform into flattened, anuclear corneocytes that constitute the cellular component of the skin barrier, the stratum corneum (SC). The SC is generally considered to be homogeneous in its structure and barrier properties, and is often shown schematically as a featureless brick wall, the “bricks” being the corneocytes, the “mortar” being intercellular lipid. Previously we showed the outer SC was not homogeneous in its composition, but contained steep gradients of the physiological inorganic elements Na, K and Cl, likely originating from sweat salts. Here we show the innermost corneocytes in human skin are also heterogeneous in composition, undergoing systematic changes in intracellular element concentration during transit into the interior of the SC.Human skin biopsies were taken from the lower leg of individuals with both “good” and “dry” skin and plunge-frozen in a stirred, cooled isopentane/propane mixture.

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