Haematology and plasma biochemistry reference intervals in wild bearded dragons ( Pogona vitticeps )

Author(s):  
JG Howard ◽  
S Jaensch
2014 ◽  
Vol 17 (8) ◽  
pp. 667-679 ◽  
Author(s):  
Randolph M Baral ◽  
Navneet K Dhand ◽  
Mark B Krockenberger ◽  
Merran Govendir

2016 ◽  
Vol 47 (2) ◽  
pp. 486-492
Author(s):  
Alexandra Scope ◽  
Ilse Schwendenwein ◽  
Gabriela Stanclova ◽  
Angela Vobornik ◽  
Richard Zink

PLoS ONE ◽  
2021 ◽  
Vol 16 (6) ◽  
pp. e0246982
Author(s):  
Carla B. Schubiger ◽  
M. Elena Gorman ◽  
Jennifer L. Johns ◽  
Mary R. Arkoosh ◽  
Joseph P. Dietrich

Plasma biochemistry and hematology reference intervals are integral health assessment tools in all medical fields, including aquatic animal health. As sablefish (Anoplopoma fimbria) are becoming aquaculturally and economically more important, this manuscript provides essential reference intervals (RI) for their plasma biochemistry and hematology along with reference photomicrographs of blood cells in healthy, fasted sablefish. Blood cell morphology can differ between fish species. In addition, blood cell counts and blood chemistry can vary between fish species, demographics, water conditions, seasons, diets, and culture systems, which precludes the use of RI’s from other fish species. For this study, blood was collected for plasma biochemistry and hematology analysis between June 20 and July 18, 2019, from healthy, yearling sablefish, hatched and reared in captivity on a commercial diet. Overnight fast of 16–18 hours did not sufficiently reduce lipids in the blood, which led to visible lipemia and frequent rupture of blood cells during analysis. Therefore, sablefish should be fasted for 24 to 36 hours before blood is collected to reduce hematology artifacts or possible reagent interference in plasma biochemistry analysis. Lymphocytes were the most dominant leukocytes (98%), while eosinophils were rare, and basophils were not detected in sablefish. Neutrophils were very large cells with Döhle bodies. In mammals and avian species, Döhle bodies are usually signs of toxic change from inflammation, but no such association was found in these fish. In conclusion, lipemia can interfere with sablefish blood analysis, and available removal methods should be evaluated as fasting for up to 36 h might not always be feasible. Also, more studies are required to establish RI for different developmental stages and rearing conditions.


2020 ◽  
Vol 51 (3) ◽  
Author(s):  
Michelle Kneeland ◽  
Emily Berman ◽  
Tiffany Grade ◽  
John Cooley ◽  
Harry Vogel ◽  
...  

2021 ◽  
Author(s):  
Carla B. Schubiger ◽  
M. Elena Gorman ◽  
Jennifer L. Johns ◽  
Mary R. Arkoosh ◽  
Joseph P. Dietrich

AbstractPlasma biochemistry and hematology reference intervals are integral health assessment tools in all medical fields, including aquatic animal health. As sablefish (Anoplopoma fimbria) are becoming aquaculturally and economically more important, this manuscript provides essential reference intervals (RI) for their plasma biochemistry and hematology along with reference photomicrographs of blood cells in healthy, fasted sablefish. Blood cell counts and morphology can vary between species, precluding the use of RI’s from other fish species for use in sablefish. Blood was collected for plasma biochemistry and hematology analysis between June 20 and July 18, 2019, from healthy, yearling sablefish, hatched and reared in captivity on a commercial diet. Overnight fast of 16-18 hours did not sufficiently reduce lipids in the blood, which lead to visible lipemia and frequent rupture of blood cells during analysis. Therefore, sablefish should be fasted for 24 to 36 hours before blood is collected to reduce hematology artifacts or possible reagent interference in plasma biochemistry analysis. RI’s may also be influenced by feed, demographics, and production systems, warranting more studies on this topic. In sablefish, lymphocytes are the dominant leukocytes (98%), while eosinophils are rare, and basophils were not detected. Neutrophils are very large cells with Döhle bodies. These bodies are usually signs of toxic changes, but no such association was found in these fish.


2016 ◽  
Vol 52 (3) ◽  
pp. 631-635 ◽  
Author(s):  
Matthew T. Hamilton ◽  
Caitlin A. Kupar ◽  
Meghan D. Kelley ◽  
John W. Finger ◽  
Tracey D. Tuberville

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