In situ ruminal degradation and in vitro fermentation characteristics, and antioxidative activities of the lotus rhizome

2019 ◽  
Vol 90 (11) ◽  
pp. 1453-1459
Author(s):  
Sanae Asano ◽  
Kohei Aoki ◽  
Yuna Kaizuka ◽  
Yuhi Kobayashi ◽  
Kenta Watanabe ◽  
...  
Keyword(s):  
In Vitro Fermentation ◽  
Ruminal Degradation

In vitro digestion and ruminal degradation of soybean hulls

2007 ◽  
Vol 2007 ◽  
pp. 166-166 ◽  
Author(s):  
Hamid Mohammadzadeh ◽  
Ali Nikkhah ◽  
Kamran Reza-Yazdi ◽  
Hassan Mehrabani-Yeganeh
Keyword(s):  
Dairy Cows ◽  
In Vitro Digestion ◽  
Soybean Hulls ◽  
Lactating Dairy Cows ◽  
Ruminal Degradation ◽  
Ruminant Animals ◽  
Soybean Processing

Dairy producers use soyhulls, a byproduct of soybean processing, to replace either grain or forage in diets of lactating dairy cows. In view of the nutritional and economical value of soyhulls it is anticipated that this practice will continue to increase in popularity among nutritionists and producers of ruminant animals. According to the NRC (2001), SH contain 60.3% NDF and 44.6% ADF on a DM basis. Also The CP content of SH averaged 11.8%, which is within the range of 13.9± 4.6%. The objectives of this paper are to evaluate the in vitro DM and OM digestibility and in situ degradability of DM, CP and NDF contents of soybean hulls.

In situ degradability of organic matter, crude protein and cell wall of various tree forages

1992 ◽  
Vol 55 (1) ◽  
pp. 29-34 ◽  
Author(s):  
P. Kamatali ◽  
E. Teller ◽  
M. Vanbelle ◽  
G. Collignon ◽  
M. Foulon
Keyword(s):  
Cell Wall ◽  
Organic Matter ◽  
Leucaena Leucocephala ◽  
Crude Protein ◽  
In Vitro Digestibility ◽  
Sesbania Sesban ◽  
Holstein Friesian ◽  
Ruminal Degradation

AbstractLeaves of Leucaena leucocephala, Sesbania sesban and Calliandra callothyrsus were harvested in Rwanda from young shoots at 8 weeks after the first cutting. They were dried, ground and incubated at the same time in polyester bags in three non-lactating Holstein-Friesian cows each fitted with a ruminal cannula. The bags were removed at 0, 2, 4, 8, 24, 48, 72 and 144 h after the start of incubations. The different parameters characterizing extent and rate of ruminal degradation of organic matter (OM), neutral-detergent fibre (NDF), and crude protein (CP) were calculated. In vitro digestibility of residual protein after 24 h and 48 h incubation was also determined. Sesbania sesban had lowest cell wall contents and gave highest ruminal degradability for OM, NDF and CP. Leucaena leucocephala was degraded to a lesser extent, but its undegraded protein had a somewhat higher in vitro digestibility. In contrast, protein of Calliandra callothyrsus was poorly degraded and digested. The proportion and composition of cell wall could not explain these differences in digestion characteristics and other measurements, such as tannins, were incriminated. Increased ruminal incubation time augmented the extent of ruminal degradation and reduced in vitro digestibility of undegraded protein but did not affect the undigestible protein fraction.

scholarly journals In situ and in vitro estimation of mineral release from common feedstuffs fed to cattle

2017 ◽  
Vol 155 (7) ◽  
pp. 1160-1173 ◽  
Author(s):  
D. ZANETTI ◽  
A. C. B. MENEZES ◽  
F. A. S. SILVA ◽  
L. F. COSTA E SILVA ◽  
P. P. ROTTA ◽  
...  
Keyword(s):  
Release Rate ◽  
Soluble Fraction ◽  
Limiting Factor ◽  
Asymptotic Model ◽  
Mineral Absorption ◽  
Large Variability ◽  
Group 4 ◽  
Ruminal Degradation

SUMMARYThe objective of the current study was to quantify the dry matter (DM) digestibility, and total ash (TA) and mineral release from 12 concentrate and 12 forage feedstuffs commonly fed to cattle using in situ and in vitro methods. Concentrate and forage feedstuffs were incubated in the rumen of ruminally cannulated beef bulls at eight different time points. Two different trials were conducted for concentrates and forages, with maximum incubation time of 72 and 120 h, respectively. The residue from samples incubated for 24 h were treated with pepsin and hydrochloric acid to simulate abomasum digestion in vitro. The initial and residual samples after in situ and in vitro incubations were measured. An asymptotic model was adopted for estimating solubility of minerals, disappearance rate of DM, and TA. Correlations between feedstuff contents and mineral release were evaluated. Residual samples from rumen fermentation after 24 h were incubated in simulated abomasal conditions and mineral release was measured. Cluster analysis was performed to group feedstuffs in relation to TA release. Large variability was observed between concentrate and forage feedstuffs for all constituents analysed. Large variability was observed for the effective ruminal degradation of TA and individual mineral release. When feedstuffs were clustered according to the immediately soluble fraction (‘a’), the insoluble by potentially releasable fraction (‘b’) and the release rate of ‘b’ (‘kd’,/h) estimates of TA ruminal release, four groups were identified. From group ‘1’ to group ‘4’, an increase in the soluble fraction and a reduction in both moderate releasable fraction and release rate was observed. Neutral detergent fibre content had a negative correlation with mineral release in the rumen, while mineral content had a positive correlation. These results demonstrate that mineral solubilization in the digestive tract is not the limiting factor for mineral absorption from the feedstuffs tested.

In vitro ruminal fermentation and in situ ruminal degradation of tamarind kernel powder extract residue in wethers

2016 ◽  
Vol 88 (7) ◽  
pp. 966-973 ◽  
Author(s):  
Lin Wang ◽  
Kazato Oishi ◽  
Yoshiaki Sato ◽  
Takashi Nakanishi ◽  
Hiroyuki Hirooka ◽  
...  
Keyword(s):  
Ruminal Fermentation ◽  
Ruminal Degradation ◽  
Tamarind Kernel ◽  
Powder Extract

In vitro fermentation and in situ rumen degradation kinetics of summer forage brassica plants

2019 ◽  
Vol 59 (7) ◽  
pp. 1271 ◽  
Author(s):  
Juan P. Keim ◽  
Jaime Cabanilla ◽  
Oscar A. Balocchi ◽  
Rubén G. Pulido ◽  
Annick Bertrand
Keyword(s):  
Chemical Composition ◽  
Degradation Kinetics ◽  
Gas Production ◽  
In Vitro Fermentation ◽  
Fermentation Products ◽  
Rumen Degradation ◽  
Kinetics Of ◽  
Forage Rape

The aim of the present study was to assess and compare the nutrient concentration, the in vitro fermentation and the in situ rumen degradation characteristics of Brassica rapa ssp. rapa L. (turnips) and Brassica napus ssp. biennis L. (forage rape). Five varieties of each species were established in three field replicates and were organised in a randomised complete-block nested design. All varieties were harvested and further analysed for chemical composition, in vitro gas-production kinetics, volatile fatty acid (VFA) production and in situ degradation kinetics of dry matter (DM) and crude protein. Turnips showed higher ash, total sugars, raffinose, sucrose, glucose and fructose concentrations (P < 0.001) than did forage rape. Turnip varieties differed in their sucrose, glucose, fructose and total soluble sugar concentration (P < 0.001), whereas rape varieties differed in their neutral detergent fibre concentration (P = 0.004) and digestible organic matter on a DM basis (P < 0.01). Regarding DM-degradation parameters, turnips had a higher soluble fraction ‘a’ (P < 0.01) and a lower insoluble, but potentially degradable fraction ‘b’ (P < 0.01) than did rape, but the fractional degradation rate ‘c’ (0.18/h) was similar to that of rape. Rates of gas production were slightly higher (P = 0.018) for turnip than for rape. No effects for brassica species nor for varieties within species were detected (P > 0.05) for total in vitro VFA production, as well as for the relative proportions of acetate, propionate, butyrate, branch chained VFA and the actetate:propionate ratio. Our study showed that most of the differences that were observed in terms of chemical composition and degradation kinetics did not result in differences in in vitro fermentation products.

The impact of particle size on the rate and extent of in vitro fermentation investigated using the Reading Pressure Technique

2000 ◽  
Vol 2000 ◽  
pp. 61-61
Author(s):  
F.L. Mould ◽  
D. Colombatto ◽  
E. Owen
Keyword(s):  
Particle Size ◽  
Pore Size ◽  
Residence Time ◽  
Fine Particles ◽  
Short Term ◽  
In Vitro Fermentation ◽  
Evaluation Systems ◽  
The Impact

The extent of rumen degradability of a feedstuff depends on the interaction between rate of degradation and residence time. In situ techniques require that substrates are ground to obtain a homogenous sample and to reduce result variability. However undegraded feed particle losses increase with bag pore size and fineness of grinding. If these particles are degraded at a similar or lower rate than the retained material, degradability, especially for short-term incubations, will be overestimated. In contrast if the feed particles lost are more readily degraded the degradability measurements obtained will be unaffected. Where improvements due to grinding have been recorded in situ these are assumed to result from variations in the proportion of fine particles that are immediately soluble or rapidly degradable. Gas-based in vitro feed evaluation systems offer the possibility of quantifying this effect directly and a study was therefore conducted to investigate the impact of particle size on the rate and extent of fermentation using the Reading Pressure Technique (Mauricio et al., 1999).

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