Effect of different methods for conserving rice grain on in situ ruminal degradation and in vivo nutrient digestion and rumen fermentation in steers

2018 ◽  
Vol 89 (7) ◽  
pp. 972-978 ◽  
Author(s):  
Makoto Miyaji ◽  
Hidehiko Inoue ◽  
Tetsuo Kawaide ◽  
Masanori Tohno ◽  
Yuko Kamiya ◽  
...  
Keyword(s):  
Rumen Fermentation ◽  
Rice Grain ◽  
Nutrient Digestion ◽  
Ruminal Degradation

Relationship between the gas production profiles of mixtures of hay and maize and the patterns of rumen fermentation observed in sheep fed those mixtures

1998 ◽  
Vol 1998 ◽  
pp. 63-63
Author(s):  
C. Rymer ◽  
D.I. Givens
Keyword(s):  
Growth Rate ◽  
Feed Intake ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Post Feeding ◽  
Molar Proportion ◽  
Rumen Ph ◽  
The Mean

The gas production (GP) technique has been developed to assess dynamics of ruminant digestion. Relationships have been observed between a feed's GP profile and in vivo parameters such as digestibility (Khazaal et al., 1993), feed intake and growth rate (Blümmel and Ørskov, 1993), and in situ degradability (Sileshi et al., 1997). However, there are few studies which relate GP data to the in vivo pattern of rumen fermentation (in terms of the rate of pH decline 2 h post-feeding and the mean rumen pH, concentration of total VFA and molar proportion of individual VFA). The object of this experiment was to determine whether such a relationship existed between a feed's GP profile and the pattern of rumen fermentation observed in animals fed that feed.

Effect of diet synchrony on rumen fermentation, production performance, immunity status and endocrine in Chinese Holstein cows

2019 ◽  
Vol 59 (4) ◽  
pp. 664 ◽  
Author(s):  
G. H. Qiao ◽  
Z. G. Xiao ◽  
Y. Li ◽  
G. J. Li ◽  
L. C. Zhao ◽  
...  
Keyword(s):  
Organic Matter ◽  
Nitrogen Concentration ◽  
Rumen Fermentation ◽  
Production Performance ◽  
Holstein Cows ◽  
Productive Performance ◽  
Chinese Holstein ◽  
Chinese Holstein Cows

In order to maximise utilisation of nutrients for ruminants, researchers directed the supply of nitrogen and organic matter synchronously to the rumen. In the current research, two experiments were conducted, one using the in situ nylon bag technique and the other using in vivo study. In Experiment 1, dynamic degradation rates of organic matter (OM) and nitrogen of each feedstuff were determined using the in situ nylon bag technique. Accordingly, three diets were formulated with the same chemical components, but differing in synchrony index (0.74, 0.85 and 0.97). Experiment 2 was subsequently carried out in vivo to evaluate the influence of diet synchrony on rumen fermentation, productive performance, immunity status and endocrine in Chinese Holstein cows. Rumen degradability of OM and neutral detergent fibre (NDF) and total tract digestibility of OM were linearly increased with the increase of synchrony index, respectively (P < 0.05). Total volatile fatty acids concentration of rumen fluid was increased linearly with the increase of synchrony index (P < 0.05). Meanwhile, rumen ammonia nitrogen concentration and blood urea nitrogen concentration were decreased in cows with the increase of synchrony index, (P < 0.05). Microbial crude protein production, the efficiency of microbial protein synthesis, and milk protein percentage, were linearly increased with the increase of synchrony index (P < 0.05). Serum concentrations of prostaglandin E2 and immunoreactive fibronectin-γ were linearly decreased with the increase of diet synchrony index (P < 0.05). The aforementioned results implicate that an increase of diet synchrony index could improve diet’s nitrogen utilisation, apparent digestibility of OM, and NDF. Additionally, it could promote rumen fermentation capacity, productive performance and immunity status in dairy cows.

The effect of dietary nitrogen and energy synchronisation on rumen fermentation and microbial protein synthesis in sheep

1992 ◽  
Vol 1992 ◽  
pp. 5-5
Author(s):  
L A. Sinclair ◽  
P. C. Gamsworthy ◽  
J. R. Newbold ◽  
P. J. Buttery
Keyword(s):  
Microbial Growth ◽  
Rumen Fermentation ◽  
Nitrogen Release ◽  
Rumen Microorganisms ◽  
Dietary Nitrogen ◽  
Diet Formulation ◽  
Micro Organisms ◽  
Current Systems

Balancing the rate of nitrogen and energy yielding substrates to rumen micro-organisms has been proposed in order to maximise the capture of rumen degradable nitrogen and to optimise microbial growth rate and efficiency (Johnson 1976). Current systems for diet formulation of ruminants are based upon the extent of fermentation of organic matter or carbohydrate fractions and the supply of rumen degradable and undegradable protein (ARC 1984, Webster, Dewhurst and Waters 1988). Little attention has been paid to the effect of the rate of supply of energy and nitrogen to rumen microorganisms in-vivo. The objectives of the current experiments were to formulate diets that had similar daily degradation of nitrogen and energy yielding components but varied in the rate of energy and nitrogen release based upon in-situ degradation characteristics of the raw ingredients, and to monitor the effect of these diets on rumen fermentation and microbial growth.

Relationship between the gas production profiles of mixtures of hay and maize and the patterns of rumen fermentation observed in sheep fed those mixtures

1998 ◽  
Vol 1998 ◽  
pp. 63-63 ◽  
Author(s):  
C. Rymer ◽  
D.I. Givens
Keyword(s):  
Growth Rate ◽  
Feed Intake ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Post Feeding ◽  
Molar Proportion ◽  
Rumen Ph ◽  
The Mean

The gas production (GP) technique has been developed to assess dynamics of ruminant digestion. Relationships have been observed between a feed's GP profile and in vivo parameters such as digestibility (Khazaal et al., 1993), feed intake and growth rate (Blümmel and Ørskov, 1993), and in situ degradability (Sileshi et al., 1997). However, there are few studies which relate GP data to the in vivo pattern of rumen fermentation (in terms of the rate of pH decline 2 h post-feeding and the mean rumen pH, concentration of total VFA and molar proportion of individual VFA). The object of this experiment was to determine whether such a relationship existed between a feed's GP profile and the pattern of rumen fermentation observed in animals fed that feed.

In vitro and in vivo polysaccharides assembly: ultrastructural and cytochemical study of growing plant cell wall components.

1978 ◽  
Vol 36 (2) ◽  
pp. 434-435
Author(s):  
D. Reis ◽  
B. Vian ◽  
J. C. Roland
Keyword(s):  
Cell Wall ◽  
Self Assembly ◽  
Plant Cell Wall ◽  
Higher Plants ◽  
Three Dimensional ◽  
Cytochemical Study ◽  
Wall Components

Wall morphogenesis in higher plants is a problem still open to controversy. Until now the possibility of a transmembrane control and the involvement of microtubules were mostly envisaged. Self-assembly processes have been observed in the case of walls of Chlamydomonas and bacteria. Spontaneous gelling interactions between xanthan and galactomannan from Ceratonia have been analyzed very recently. The present work provides indications that some processes of spontaneous aggregation could occur in higher plants during the formation and expansion of cell wall.Observations were performed on hypocotyl of mung bean (Phaseolus aureus) for which growth characteristics and wall composition have been previously defined.In situ, the walls of actively growing cells (primary walls) show an ordered three-dimensional organization (fig. 1). The wall is typically polylamellate with multifibrillar layers alternately transverse and longitudinal. Between these layers intermediate strata exist in which the orientation of microfibrils progressively rotates. Thus a progressive change in the morphogenetic activity occurs.

Three-Dimensional Subcellular Organization of Hepatocytes in Intact Liver: Simultaneous Visualization of Cytoskeletal and Membrane Markers by Confocal Microscopy

1996 ◽  
Vol 54 ◽  
pp. 288-289
Author(s):  
Greg V. Martin ◽  
Ann L. Hubbard
Keyword(s):  
Three Dimensional ◽  
Integral Membrane Proteins ◽  
Polarized Secretion ◽  
Microscope Images ◽  
Computer Aided ◽  
Intracellular Organelles ◽  
Cytoskeletal Elements ◽  
Simultaneous Visualization

The microtubule (MT) cytoskeleton is necessary for many of the polarized functions of hepatocytes. Among the functions dependent on the MT-based cytoskeleton are polarized secretion of proteins, delivery of endocytosed material to lysosomes, and transcytosis of integral plasma membrane (PM) proteins. Although microtubules have been shown to be crucial to the establishment and maintenance of functional and structural polarization in the hepatocyte, little is known about the architecture of the hepatocyte MT cytoskeleton in vivo, particularly with regard to its relationship to PM domains and membranous organelles. Using an in situ extraction technique that preserves both microtubules and cellular membranes, we have developed a protocol for immunofluorescent co-localization of cytoskeletal elements and integral membrane proteins within 20 µm cryosections of fixed rat liver. Computer-aided 3D reconstruction of multi-spectral confocal microscope images was used to visualize the spatial relationships among the MT cytoskeleton, PM domains and intracellular organelles.

Evaluation of Structure-Controlled Silk Fibroin Coatings for Orthopedic Infection and In-Situ Osteogenesis: In Vitro and In Vivo

2020 ◽  
Author(s):  
Wenhao Zhou ◽  
Teng Zhang ◽  
Jianglong Yan ◽  
QiYao Li ◽  
Panpan Xiong ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Orthopedic Infection

scholarly journals Ferromagnetic soft catheter robots for minimally invasive bioprinting

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Cheng Zhou ◽  
Youzhou Yang ◽  
Jiaxin Wang ◽  
Qingyang Wu ◽  
Zhuozhi Gu ◽  
...  
Keyword(s):  
Minimally Invasive ◽  
The Body ◽  
Magnetic Actuation ◽  
Internal Organs ◽  
Planar Surfaces ◽  
Reinforced Polymer ◽  
Direct Fabrication ◽  
Digitally Controlled

AbstractIn vivo bioprinting has recently emerged as a direct fabrication technique to create artificial tissues and medical devices on target sites within the body, enabling advanced clinical strategies. However, existing in vivo bioprinting methods are often limited to applications near the skin or require open surgery for printing on internal organs. Here, we report a ferromagnetic soft catheter robot (FSCR) system capable of in situ computer-controlled bioprinting in a minimally invasive manner based on magnetic actuation. The FSCR is designed by dispersing ferromagnetic particles in a fiber-reinforced polymer matrix. This design results in stable ink extrusion and allows for printing various materials with different rheological properties and functionalities. A superimposed magnetic field drives the FSCR to achieve digitally controlled printing with high accuracy. We demonstrate printing multiple patterns on planar surfaces, and considering the non-planar surface of natural organs, we then develop an in situ printing strategy for curved surfaces and demonstrate minimally invasive in vivo bioprinting of hydrogels in a rat model. Our catheter robot will permit intelligent and minimally invasive bio-fabrication.

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