Effect of bromochloromethane and fumarate on phylogenetic diversity of the formyltetrahydrofolate synthetase gene in bovine rumen

2013 ◽  
Vol 85 (1) ◽  
pp. 25-31 ◽  
Author(s):  
Makoto Mitsumori ◽  
Hiroki Matsui ◽  
Kiyoshi Tajima ◽  
Takumi Shinkai ◽  
Akio Takenaka ◽  
...  
Keyword(s):  
Phylogenetic Diversity ◽  
Formyltetrahydrofolate Synthetase ◽  
Bovine Rumen

scholarly journals Functional Gene Analysis Suggests Different Acetogen Populations in the Bovine Rumen and Tammar Wallaby Forestomach

2010 ◽  
Vol 76 (23) ◽  
pp. 7785-7795 ◽  
Author(s):  
Emma J. Gagen ◽  
Stuart E. Denman ◽  
Jagadish Padmanabha ◽  
Someshwar Zadbuke ◽  
Rafat Al Jassim ◽  
...  
Keyword(s):  
Pcr Primers ◽  
Tammar Wallaby ◽  
Amino Acid Sequences ◽  
Functional Gene ◽  
Acetyl Coa ◽  
Formyltetrahydrofolate Synthetase ◽  
Bovine Rumen ◽  
Specific Pcr ◽  
Acetyl Coa Pathway ◽  
Functional Gene Analysis

ABSTRACT Reductive acetogenesis via the acetyl coenzyme A (acetyl-CoA) pathway is an alternative hydrogen sink to methanogenesis in the rumen. Functional gene-based analysis is the ideal approach for investigating organisms capable of this metabolism (acetogens). However, existing tools targeting the formyltetrahydrofolate synthetase gene (fhs) are compromised by lack of specificity due to the involvement of formyltetrahydrofolate synthetase (FTHFS) in other pathways. Acetyl-CoA synthase (ACS) is unique to the acetyl-CoA pathway and, in the present study, acetyl-CoA synthase genes (acsB) were recovered from a range of acetogens to facilitate the design of acsB-specific PCR primers. fhs and acsB libraries were used to examine acetogen diversity in the bovine rumen and forestomach of the tammar wallaby (Macropus eugenii), a native Australian marsupial demonstrating foregut fermentation analogous to rumen fermentation but resulting in lower methane emissions. Novel, deduced amino acid sequences of acsB and fhs affiliated with the Lachnospiraceae in both ecosystems and the Ruminococcaeae/Blautia group in the rumen. FTHFS sequences that probably originated from nonacetogens were identified by low “homoacetogen similarity” scores based on analysis of FTHFS residues, and comprised a large proportion of FTHFS sequences from the tammar wallaby forestomach. A diversity of FTHFS and ACS sequences in both ecosystems clustered between the Lachnospiraceae and Clostridiaceae acetogens but without close sequences from cultured isolates. These sequences probably originated from novel acetogens. The community structures of the acsB and fhs libraries from the rumen and the tammar wallaby forestomach were different (LIBSHUFF, P < 0.001), and these differences may have significance for overall hydrogenotrophy in both ecosystems.

scholarly journals Plane of nutrition affects the phylogenetic diversity and relative abundance of transcriptionally active methanogens in the bovine rumen

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Emily McGovern ◽  
Matthew S. McCabe ◽  
Paul Cormican ◽  
Milka Popova ◽  
Kate Keogh ◽  
...  
Keyword(s):  
Relative Abundance ◽  
Phylogenetic Diversity ◽  
Bovine Rumen

Lactic acid in the bovine rumen

1966 ◽  
Author(s):  
Jerry Vern De Barthe
Keyword(s):  
Lactic Acid ◽  
Bovine Rumen

Phylogenetic Diversity of Rhizobial Bacteria Isolated from Astragalus sinicus*

2010 ◽  
Vol 16 (3) ◽  
pp. 380-384 ◽  
Author(s):  
Xiaoxia ZHANG ◽  
Xiaotong MA ◽  
Weidong CAO ◽  
Shanjun WEI ◽  
Jianhui CAI ◽  
...  
Keyword(s):  
Phylogenetic Diversity ◽  
Astragalus Sinicus
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