The use of dietary oak acorn extract to improve haematological parameters, mucosal and serum immunity, skin mucus bactericidal activity, and disease resistance in rainbow trout ( Oncorhynchus mykiss )

2021 ◽  
Author(s):  
Hamed Ghafarifarsani ◽  
Ghasem Rashidian ◽  
Atefeh Sheikhlar ◽  
Mehdi Naderi Farsani ◽  
Seyed Hossein Hoseinifar ◽  
...  
2016 ◽  
Vol 48 (6) ◽  
pp. 2672-2682 ◽  
Author(s):  
Youngjin Park ◽  
Seunghan Lee ◽  
Jeongwhui Hong ◽  
Doyoung Kim ◽  
Mohammad Moniruzzaman ◽  
...  

2008 ◽  
Vol 411 (1) ◽  
pp. 33-40 ◽  
Author(s):  
Virginie Molle ◽  
Sylvie Campagna ◽  
Yannick Bessin ◽  
Nathalie Ebran ◽  
Nathalie Saint ◽  
...  

The epidermis of fish is covered with a layer of mucus, which contributes to the defence of the species against parasites, bacteria and fungi. We have previously extracted glycoproteins from various mucus samples from fish and have shown that they present pore-forming activities well correlated with strong antibacterial properties [Ebran, Julien, Orange, Saglio, Lemaitre and Molle (2000) Biochim. Biophys. Acta 1467, 271–280]. The present study focuses on the 65 kDa glycoprotein, Tr65, from the rainbow trout (Oncorhynchus mykiss, formerly Salmo gairdneri). Enzymatic digestion of Tr65 yielded a fragment pattern with strong homology with that of trout type II cytokeratin. Sequence analysis of the cDNA clone obtained by PCR confirmed this homology. We thus constructed a plasmid to overproduce the recombinant Tr65. We extracted and purified this recombinant Tr65, using it for multichannel and single-channel experiments in azolectin bilayers. Our results with recombinant Tr65 confirmed the pore-forming properties already shown with native antibacterial Tr65. These findings offer new insights into the function of keratin proteins present in various mucosal surfaces of animals and human beings.


Author(s):  
Ünal İspir ◽  
Mikail Özcan

Rainbow trout (Oncorhynchus mykiss) were examined effects of apricot kernel oil (Prunus armeniaca L.) on the immune mechanisms. After Fish weight 40 ±0,03 g was intraperitoneal (i.p.) injection 1% and 10% apricot kernel oil (AKO), blood was taken from the caudal vein of anesthetized (50 ppm, benzocaine) fish. Myeloperoxidase production (MPO), Serum bactericidal activity, protein levels, Lysozyme response (LYS) and total immunoglobulin (Ig) were determined on days 3, 7, 14 and 21. The same strategy was conducted on a control bunch. No contrasts were found within the levels of immunuglobulin between the control and test groups. However, there were considerable increases in bactericidal activity, MPO, LYS, protein levels and important differences were detected between the control and experimental groups. AKO can be utilized to improve the resistant instruments of rainbow trout.


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