Analysis of viral load between different tissues and rate of progression of white spot syndrome virus (WSSV) inPenaeus monodon

2013 ◽  
Vol 46 (8) ◽  
pp. 2003-2012 ◽  
Author(s):  
Joseph Jeswin ◽  
Antony Anju ◽  
Palahani Chacko Thomas ◽  
Meleth Porinchu Paulton ◽  
Koyadan Kizhakedath Vijayan
Keyword(s):  
Viral Load ◽  
White Spot Syndrome Virus ◽  
White Spot ◽  
Rate Of Progression ◽  
White Spot Syndrome ◽  
Different Tissues

scholarly journals Sulfated galactans isolated from the red seaweed Gracilaria fisheri target the envelope proteins of white spot syndrome virus and protect against viral infection in shrimp haemocytes

2014 ◽  
Vol 95 (5) ◽  
pp. 1126-1134 ◽  
Author(s):  
Tawut Rudtanatip ◽  
Somluk Asuvapongpatana ◽  
Boonsirm Withyachumnarnkul ◽  
Kanokpan Wongprasert
Keyword(s):  
Viral Load ◽  
Viral Infection ◽  
White Spot Syndrome Virus ◽  
Penaeus Monodon ◽  
White Spot ◽  
Red Seaweed ◽  
Viral Attachment ◽  
Sulfated Galactans ◽  
Cell Mortality ◽  
White Spot Syndrome

The present study was aimed at evaluating an underlying mechanism of the antiviral activity of the sulfated galactans (SG) isolated from the red seaweed Gracilaria fisheri against white spot syndrome virus (WSSV) infection in haemocytes of the black tiger shrimp Penaeus monodon. Primary culture of haemocytes from Penaeus monodon was performed and inoculated with WSSV, after which the cytopathic effect (CPE), cell viability and viral load were determined. Haemocytes treated with WSSV-SG pre-mix showed decreased CPE, viral load and cell mortality from the viral infection. Solid-phase virus-binding assays revealed that SG bound to WSSV in a dose-related manner. Far Western blotting analysis indicated that SG bound to VP 26 and VP 28 proteins of WSSV. In contrast to the native SG, desulfated SG did not reduce CPE and cell mortality, and showed low binding activity with WSSV. The current study suggests that SG from Gracilaria fisheri elicits its anti-WSSV activity by binding to viral proteins that are important for the process of viral attachment to the host cells. It is anticipated that the sulfate groups of SG are important for viral binding.

scholarly journals Hyperthermia reduces viral load of white spot syndrome virus in Penaeus vannamei

2006 ◽  
Vol 68 ◽  
pp. 175-180 ◽  
Author(s):  
CB Granja ◽  
OM Vidal ◽  
G Parra ◽  
M Salazar
Keyword(s):  
Viral Load ◽  
White Spot Syndrome Virus ◽  
White Spot ◽  
Penaeus Vannamei ◽  
White Spot Syndrome

White spot syndrome virus in decapods from Mississippi Sound, USA, and susceptibility of Palaemonetes pugio and Uca panacea to a Chinese isolate

2020 ◽  
Vol 138 ◽  
pp. 121-131
Author(s):  
M Muhammad ◽  
JM Lotz ◽  
RB Blaylock ◽  
SS Curran
Keyword(s):  
Viral Load ◽  
Native Species ◽  
White Spot Syndrome Virus ◽  
Reservoir Host ◽  
White Spot ◽  
Chinese Isolate ◽  
Palaemonetes Pugio ◽  
Mississippi Sound ◽  
Total Dna ◽  
White Spot Syndrome

The presence and quantity of white spot syndrome virus (WSSV) was surveyed using TaqMan real-time PCR to assess the extent of the virus in Mississippi Sound, USA. A total of 3577 wild decapods comprising 11 species was collected between November 2012 and August 2015: WSSV was present in 10 of the 11 species. Prevalence ranged from 5.1% in Uca rapax to 38.8% in U. spinicarpa. Viral load ranged from 1.8 to 7.3 log10 copies of WSSV µg-1 total DNA. Two Gulf species, Palaemonetes pugio and U. panacea, were injected with a series of doses of a virulent WSSV isolate from China to determine relative susceptibility and virulence because continuing translocation of highly pathogenic isolates of WSSV poses risk to native species. Survival was 0-65% for P. pugio and 5-60% for U. panacea. Median survival time was lower for P. pugio than U. panacea at all doses. Mean (±SD) lethal load was 9.0 ± 8.9 log copies of WSSV µg-1 total DNA in P. pugio and 8.2 ± 8.3 in U. panacea. Mean viral load in survivors was higher in U. panacea than in P. pugio (5.8 ± 6.1 vs. 3.2 ± 3.0 log copies of WSSV µg-1 total DNA); mean viral load was lower in wild individuals of those species (2.9 ± 3.2 for P. pugio and 4.9 ± 5.0 for U. panacea). U. panacea is potentially more tolerant of WSSV than P. pugio and may serve as an important reservoir host in the community.

Water temperature influences viral load and detection of White Spot Syndrome Virus (WSSV) in Litopenaeus vannamei and wild crustaceans

Aquaculture ◽  
2012 ◽  
Vol 326-329 ◽  
pp. 9-14 ◽  
Author(s):  
Juliana R. Moser ◽  
Diego A. Galván Álvarez ◽  
Fernando Mendoza Cano ◽  
Trinidad Encinas Garcia ◽  
Daniel E. Coronado Molina ◽  
...  
Keyword(s):  
Viral Load ◽  
Water Temperature ◽  
Litopenaeus Vannamei ◽  
White Spot Syndrome Virus ◽  
White Spot ◽  
White Spot Syndrome
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