scholarly journals Hyperthermia reduces viral load of white spot syndrome virus in Penaeus vannamei

2006 ◽  
Vol 68 ◽  
pp. 175-180 ◽  
Author(s):  
CB Granja ◽  
OM Vidal ◽  
G Parra ◽  
M Salazar
Keyword(s):  
Viral Load ◽  
White Spot Syndrome Virus ◽  
White Spot ◽  
Penaeus Vannamei ◽  
White Spot Syndrome

scholarly journals Sulfated galactans isolated from the red seaweed Gracilaria fisheri target the envelope proteins of white spot syndrome virus and protect against viral infection in shrimp haemocytes

2014 ◽  
Vol 95 (5) ◽  
pp. 1126-1134 ◽  
Author(s):  
Tawut Rudtanatip ◽  
Somluk Asuvapongpatana ◽  
Boonsirm Withyachumnarnkul ◽  
Kanokpan Wongprasert
Keyword(s):  
Viral Load ◽  
Viral Infection ◽  
White Spot Syndrome Virus ◽  
Penaeus Monodon ◽  
White Spot ◽  
Red Seaweed ◽  
Viral Attachment ◽  
Sulfated Galactans ◽  
Cell Mortality ◽  
White Spot Syndrome

The present study was aimed at evaluating an underlying mechanism of the antiviral activity of the sulfated galactans (SG) isolated from the red seaweed Gracilaria fisheri against white spot syndrome virus (WSSV) infection in haemocytes of the black tiger shrimp Penaeus monodon. Primary culture of haemocytes from Penaeus monodon was performed and inoculated with WSSV, after which the cytopathic effect (CPE), cell viability and viral load were determined. Haemocytes treated with WSSV-SG pre-mix showed decreased CPE, viral load and cell mortality from the viral infection. Solid-phase virus-binding assays revealed that SG bound to WSSV in a dose-related manner. Far Western blotting analysis indicated that SG bound to VP 26 and VP 28 proteins of WSSV. In contrast to the native SG, desulfated SG did not reduce CPE and cell mortality, and showed low binding activity with WSSV. The current study suggests that SG from Gracilaria fisheri elicits its anti-WSSV activity by binding to viral proteins that are important for the process of viral attachment to the host cells. It is anticipated that the sulfate groups of SG are important for viral binding.

scholarly journals Protector effect of beta-glucans from shrimp pond-related yeasts in Penaeus vannamei rearing under white spot syndrome virus presence

2021 ◽  
Vol 49 (1) ◽  
pp. 18-28
Author(s):  
Norma A. Ochoa-Álvarez ◽  
Ramón Casillas-Hernández ◽  
Francisco J. Magallón-Barajas ◽  
Jesús M. Ramirez-Orozco ◽  
Elizabeth Carbajal-Millán
Keyword(s):  
Protective Effect ◽  
White Spot Syndrome Virus ◽  
White Spot ◽  
Proton Nuclear Magnetic Resonance ◽  
Penaeus Vannamei ◽  
Marine Yeasts ◽  
Shrimp Pond ◽  
Beta Glucans ◽  
White Spot Syndrome ◽  
Excellent Source

This research study tested the protective effect of prolonged exposure of shrimp food supplemented with glucans from shrimp-pond related yeasts on shrimp Penaeus vannamei reared under the presence of the white spot syndrome virus (WSSV). The glucans extracted and purified from isolated marine yeasts identified as Debaryomyces hansenii, Candida tropicalis, Candida humilis, Candida glabrata, Pichia kudriavzevyi, Wickerhamomyces anomalus and the terrestrial Saccharomyces cerevisiae yeast were characterized by the Fourier-transform infrared spectroscopy and proton nuclear magnetic resonance spectroscopy. The treatments were prepared with food enriched with the yeast beta-glucans and the control groups without beta-glucans. Shrimp were fed thrice a day and challenged orally with WSSV on days 31, 54, 66 and muscularly at day 70. The animals were assessed for the protective effect in terms of post-infection total hemocyte counts, and survival rate. The results indicated that marine yeasts possessed ß-1,3/1,6-glucans, and that D. hansenii was an excellent source yielding 30% of its dry biomass of pure glucans. For the positive control group where no glucans were added, WSSV challenges showed 100% survival when the virus was provided orally, and 40% when the virus was injected. These results also indicated that the shrimp line selected for this study was a resistant line for WSSV. Shrimp groups fed with glucans of the marine yeasts D. hansenii and C. humillis showed a significant protection, allowing shrimp survival of 66% while terrestrial yeast showed 57.14%. These results indicated that marine yeasts growing in the shrimp pond were an excellent source of beta-glucans that allowed extra protection against the mortality caused by this pathogenic virus.

scholarly journals The effect of raising water temperature to 33 °C in Penaeus vannamei juveniles at different stages of infection with white spot syndrome virus (WSSV)

Aquaculture ◽  
2007 ◽  
Vol 272 (1-4) ◽  
pp. 240-245 ◽  
Author(s):  
M.M. Rahman ◽  
M. Corteel ◽  
M. Wille ◽  
V. Alday-Sanz ◽  
M.B. Pensaert ◽  
...  
Keyword(s):  
Water Temperature ◽  
White Spot Syndrome Virus ◽  
White Spot ◽  
Penaeus Vannamei ◽  
White Spot Syndrome
Sign in / Sign up

Export Citation Format

Share Document