Characterization of a lytic EBP bacteriophage with large size genome against Enterobacter cloacae

Apmis ◽  
2021 ◽  
Author(s):  
Muhammad Asif ◽  
Hafsa Naseem ◽  
Iqbal Ahmed Alvi ◽  
Abdul Basit ◽  
Shafiq‐ur‐Rehman
Keyword(s):  
Enterobacter Cloacae ◽  
Large Size

scholarly journals Characterization of resistance mechanisms of Enterobacter cloacae Complex co-resistant to carbapenem and colistin

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Shixing Liu ◽  
Renchi Fang ◽  
Ying Zhang ◽  
Lijiang Chen ◽  
Na Huang ◽  
...  
Keyword(s):  
Lipid A ◽  
Efflux Pump ◽  
Resistance Mechanism ◽  
Carbapenem Resistance ◽  
Enterobacter Cloacae ◽  
Resistance Mechanisms ◽  
Colistin Resistance ◽  
Carbapenem Resistant ◽  
Horizontal Spread

Abstract Background The emergence of carbapenem-resistant and colistin-resistant ECC pose a huge challenge to infection control. The purpose of this study was to clarify the mechanism of the carbapenems and colistin co-resistance in Enterobacter cloacae Complex (ECC) strains. Results This study showed that the mechanisms of carbapenem resistance in this study are: 1. Generating carbapenemase (7 of 19); 2. The production of AmpC or ESBLs combined with decreased expression of out membrane protein (12 of 19). hsp60 sequence analysis suggested 10 of 19 the strains belong to colistin hetero-resistant clusters and the mechanism of colistin resistance is increasing expression of acrA in the efflux pump AcrAB-TolC alone (18 of 19) or accompanied by a decrease of affinity between colistin and outer membrane caused by the modification of lipid A (14 of 19). Moreover, an ECC strain co-harboring plasmid-mediated mcr-4.3 and blaNDM-1 has been found. Conclusions This study suggested that there is no overlap between the resistance mechanism of co-resistant ECC strains to carbapenem and colistin. However, the emergence of strain co-harboring plasmid-mediated resistance genes indicated that ECC is a potential carrier for the horizontal spread of carbapenems and colistin resistance.

scholarly journals Prevalence and characterization of extended spectrum beta-lactamase-producing Enterobacter cloacae strains in Algeria

2013 ◽  
Vol 7 (11) ◽  
pp. 804-811 ◽  
Author(s):  
Sabrina Nedjai ◽  
Abouddihaj Barguigua ◽  
Nassima Djahmi ◽  
Loubna Jamali ◽  
Khalid Zerouali ◽  
...  
Keyword(s):  
Enterobacter Cloacae ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Extended Spectrum Beta Lactamase ◽  
Agar Dilution ◽  
Synergy Test ◽  
High Level ◽  
Genomic Typing ◽  
Microbiological Data

Introduction: Expended spectrum β-lactamase (ESBL)-producing Enterobacter cloacae is an important nosocomial pathogen. In this study, the prevalence and the molecular epidemiology of ESBL producing E. cloacae strains isolated from various hospitals in Annaba, Algeria were investigated. Methodology: The study involved 63 isolates of E. cloacae obtained during 2009 at the four hospitals in Annaba. The detection of ESBL was performed using the double-disk synergy test and the combined disk test. Minimum inhibitory concentrations (MICs) were determined using the agar dilution method. The presence of blaCTX-M, blaSHV, blaTEM, and blaDHA β-lactamase genes was evaluated by PCR, and genomic typing was determined by pulsed-field gel electrophoresis (PFGE) analysis. The clinical and microbiological data were entered into the EpiI Info database. Results: Thirty isolates (47.6%) had an ESBL phenotype. BlaCTX-M group1 (76%); blaTEM (70%) were the most prevalent, followed by blaDHA (16.6%) and blaSHV (10%). Eighteen strains expressed at least two bla genes. MICs revealed a high level of resistance to cefotaxime, ceftazidime, and cefepime.  PFGE revealed an epidemic clonal dissemination of these isolates. Various risk factors associated with the occurrence of ESBL-producing E. cloacae were detected. Conclusions: A higher frequency of ESBL-producing isolates and a diversity of β-lactamases were detected among ESBL-producing E. cloacae; these resulted from an epidemic clonal dissemination and high transference of ESBL genes between bacteria in hospital settings. Strict measures will be required to control the further spread of these pathogens in hospital settings.

Growth and characterization of large-size bismuth germanate single crystals by low thermal gradient Czochralski method

1999 ◽  
Vol 197 (4) ◽  
pp. 865-873 ◽  
Author(s):  
R.V Anantha Murthy ◽  
M Ravikumar ◽  
A Choubey ◽  
Krishan Lal ◽  
Lyudmila Kharachenko ◽  
...  
Keyword(s):  
Single Crystals ◽  
Thermal Gradient ◽  
Czochralski Method ◽  
Bismuth Germanate ◽  
Large Size

scholarly journals Growth and Characterization of Epsomite Single Crystals Doped with KCl from low Temperature Aqueous Solutions

1970 ◽  
Vol 33 (1) ◽  
pp. 47-54 ◽  
Author(s):  
S Ferdous ◽  
J Podder
Keyword(s):  
Aqueous Solutions ◽  
Single Crystals ◽  
Optical Quality ◽  
Dielectric Studies ◽  
Slow Cooling ◽  
Large Size ◽  
Isothermal Evaporation ◽  
Academy Of Sciences

Highly transparent and well faceted large size epsomite single crystals have been grown in pure form and doped with KCl from aqueous solutions by slow cooling and isothermal evaporation method. The optical quality of the epsomite improves on doping by KCl. Mass growth rates were found to increase with doping of lower concentrations of KCl and then decreases with the higher concentration of KCl. KCl doped epsomite crystal reveals that structures are slightly distorted due to adsorption of Cl- ion into the crystal lattice. DC conductivity along the growth axis for all of the grown crystals increases with temperature in the range of 25 to 70ºC and also increases with the KCl concentration. Dielectric constant is found to be almost independent of frequency up to range of 106Hz. The dielectric studies show the suitability of these grown crystals for optoelectronic applications. DOI: 10.3329/jbas.v33i1.2949 Journal of Bangladesh Academy of Sciences, Vol. 33, No. 1, 47-54, 2009

scholarly journals Molecular characterization of an Enterobacter cloacae outer membrane protein (OmpX).

1991 ◽  
Vol 173 (1) ◽  
pp. 156-160 ◽  
Author(s):  
J Stoorvogel ◽  
M J van Bussel ◽  
J Tommassen ◽  
J A van de Klundert
Keyword(s):  
Membrane Protein ◽  
Outer Membrane ◽  
Molecular Characterization ◽  
Outer Membrane Protein ◽  
Enterobacter Cloacae

scholarly journals Molecular Characterization of Carbapenem-Resistant Enterobacter cloacae in 11 Chinese Cities

2018 ◽  
Vol 9 ◽  
Author(s):  
Chunmei Jin ◽  
Jiangang Zhang ◽  
Qi Wang ◽  
Hongbin Chen ◽  
Xiaojuan Wang ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Enterobacter Cloacae ◽  
Chinese Cities ◽  
Carbapenem Resistant

Characterization of Enterobacter cloacae BAGM01 Producing a Thermostable and Alkaline-Tolerant Rhamnolipid Biosurfactant from the Gulf of Mexico

2020 ◽  
Author(s):  
Nidya Fabiola Curiel-Maciel ◽  
Fernando Martínez-Morales ◽  
Alexei Fedorovish Licea-Navarro ◽  
Brandt Bertrand ◽  
A. Berenice Aguilar-Guadarrama ◽  
...  
Keyword(s):  
Gulf Of Mexico ◽  
Enterobacter Cloacae ◽  
Rhamnolipid Biosurfactant ◽  
Alkaline Tolerant

Characterization of NDM-1- and CMH-3-producing Enterobacter cloacae complex ST932 in a patient transferred from Ukraine to Spain

2020 ◽  
Vol 38 (7) ◽  
pp. 327-330
Author(s):  
Inmaculada López-Hernández ◽  
Aurora García Barrionuevo ◽  
Paula Díaz de Alba ◽  
Encarnación Clavijo ◽  
Alvaro Pascual
Keyword(s):  
Enterobacter Cloacae ◽  
Enterobacter Cloacae Complex

scholarly journals Characterization of the Enterobacter Phage vB_EclM_CIP9

2020 ◽  
Vol 9 (13) ◽  
Author(s):  
Klara Wang ◽  
Marielou G. Tamayo ◽  
Tiffany V. Penner ◽  
Bradley W. M. Cook ◽  
Deborah A. Court ◽  
...  
Keyword(s):  
Enterobacter Cloacae ◽  
Opportunistic Pathogen ◽  
Multidrug Resistant ◽  
Open Reading Frames ◽  
Resistant Isolate ◽  
Content Type ◽  
Hospital Acquired Infections ◽  
Hospital Acquired ◽  
Reading Frames

Enterobacter cloacae is an opportunistic pathogen that causes hospital-acquired infections in immunocompromised patients. Here, we describe vB_EclM_CIP9, a novel Enterobacter phage that infects a multidrug-resistant isolate of E. cloacae. Phage vB_EclM_CIP9 is a myovirus that has a 174,924-bp genome, with 296 predicted open reading frames.

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