Nuclear and mitochondrial DNAs microsatellite instability and mitochondrial DNA copy number in adenocarcinoma and squamous cell carcinoma of lung: a pilot study

Apmis ◽  
2015 ◽  
Vol 123 (12) ◽  
pp. 1048-1054 ◽  
Author(s):  
Deok Heon Lee ◽  
Jae-Ho Lee ◽  
Dae-Kwang Kim ◽  
Dong Yoon Keum
Keyword(s):  
Squamous Cell Carcinoma ◽  
Mitochondrial Dna ◽  
Pilot Study ◽  
Microsatellite Instability ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Copy Number ◽  
Dna Copy Number ◽  
Mitochondrial Dna Copy Number ◽  
Mitochondrial Dnas

scholarly journals Copy number gains of the putative CRKL oncogene in laryngeal squamous cell carcinoma result in strong nuclear expression of the protein and influence cell proliferation and migration

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Magdalena Kostrzewska-Poczekaj ◽  
Kinga Bednarek ◽  
Malgorzata Jarmuz-Szymczak ◽  
Magdalena Bodnar ◽  
Violeta Filas ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Cell Lines ◽  
Squamous Cell ◽  
Copy Number ◽  
Laryngeal Squamous Cell Carcinoma ◽  
Dna Copy Number ◽  
Nuclear Expression ◽  
And Migration ◽  
Proliferation And Migration

AbstractLaryngeal squamous cell carcinoma is a major medical problem worldwide. Although our understanding of genetic changes and their consequences in laryngeal cancer has opened new therapeutic pathways over the years, the diagnostic as well as treatment options still need to be improved. In our previous study, we identified CRKL (22q11) as a novel putative oncogene overexpressed and amplified in a subset of LSCC tumors and cell lines. Here we analyze to what extent CRKL DNA copy number gains correlate with the higher expression of CRKL protein by performing IHC staining of the respective protein in LSCC cell lines (n = 3) and primary tumors (n = 40). Moreover, the importance of CRKL gene in regard to proliferation and motility of LSCC cells was analyzed with the application of RNA interference (siRNA). Beside the physiological cytoplasmic expression, the analysis of LSCC tumor samples revealed also nuclear expression of CRKL protein in 10/40 (25%) cases, of which three (7.5%), presented moderate or strong nuclear expression. Similarly, we observed a shift towards aberrantly strong nuclear abundance of the CRKL protein in LSCC cell lines with gene copy number amplifications. Moreover, siRNA mediated silencing of CRKL gene in the cell lines showing its overexpression, significantly reduced proliferation (p < 0.01) as well as cell migration (p < 0.05) rates. Altogether, these results show that the aberrantly strong nuclear localization of CRKL is a seldom but recurrent phenomenon in LSCC resulting from the increased DNA copy number and overexpression of the gene. Moreover, functional analyses suggest that proliferation and migration of the tumor cells depend on CRKL expression.

scholarly journals Integrative Analysis in Oral Squamous Cell Carcinoma Reveals DNA Copy Number–Associated miRNAs Dysregulating Target Genes

2012 ◽  
Vol 147 (3) ◽  
pp. 501-508 ◽  
Author(s):  
Nicholas A. Serrano ◽  
Chang Xu ◽  
Yan Liu ◽  
Pei Wang ◽  
Wenhong Fan ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Copy Number ◽  
Target Genes ◽  
Integrative Analysis ◽  
Dna Copy Number

scholarly journals Role of Mitochondrial DNA Copy Number Alteration in Human Renal Cell Carcinoma

2016 ◽  
Vol 17 (6) ◽  
pp. 814 ◽  
Author(s):  
Chen-Sung Lin ◽  
Hui-Ting Lee ◽  
Ming-Huei Lee ◽  
Siao-Cian Pan ◽  
Chen-Yeh Ke ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Mitochondrial Dna ◽  
Cell Carcinoma ◽  
Renal Cell ◽  
Copy Number ◽  
Copy Number Alteration ◽  
Dna Copy Number ◽  
Human Renal Cell Carcinoma ◽  
Mitochondrial Dna Copy Number
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