scholarly journals Role of Mitochondrial DNA Copy Number Alteration in Human Renal Cell Carcinoma

2016 ◽  
Vol 17 (6) ◽  
pp. 814 ◽  
Author(s):  
Chen-Sung Lin ◽  
Hui-Ting Lee ◽  
Ming-Huei Lee ◽  
Siao-Cian Pan ◽  
Chen-Yeh Ke ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Mitochondrial Dna ◽  
Cell Carcinoma ◽  
Renal Cell ◽  
Copy Number ◽  
Copy Number Alteration ◽  
Dna Copy Number ◽  
Human Renal Cell Carcinoma ◽  
Mitochondrial Dna Copy Number

scholarly journals A Case-Control Study of Peripheral Blood Mitochondrial DNA Copy Number and Risk of Renal Cell Carcinoma

PLoS ONE ◽  
2012 ◽  
Vol 7 (8) ◽  
pp. e43149 ◽  
Author(s):  
Mark P. Purdue ◽  
Jonathan N. Hofmann ◽  
Joanne S. Colt ◽  
Mirjam Hoxha ◽  
Julie J. Ruterbusch ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Mitochondrial Dna ◽  
Cell Carcinoma ◽  
Peripheral Blood ◽  
Renal Cell ◽  
Copy Number ◽  
Case Control Study ◽  
Case Control ◽  
Mitochondrial Dna Copy Number ◽  
Control Study

scholarly journals SPOP Could Play a Potential Inhibitory Role in Human Renal Cell Carcinoma

2021 ◽  
Author(s):  
zhi chen ◽  
Zuan Li ◽  
Deyong Nong ◽  
Ximing Li ◽  
Guihai Huang ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Cell Lines ◽  
Renal Cell ◽  
Immunohistochemical Staining ◽  
Human Renal Cell Carcinoma ◽  
Expression Levels ◽  
Normal Tissues ◽  
Proliferation And Apoptosis

Abstract Background: SPOP, a substrate adaptor of Cul3 ubiquitin ligase, plays crucial roles in solid neoplasms by promoting the ubiquitination and degradation of substrates. Limited studies have shown that SPOP is overexpressed in human renal cell carcinoma (RCC) tissue. However, the exact role of SPOP in RCC remains unclear and needs to be further elucidated. The present study showed that SPOP was expressed at different levels in different RCC cell lines. The purpose of this study was to explore the roles of SPOP in the biological features of RCC cells and determine the expression levels of SPOP in human tissue microarrays (TMAs) and kidney tissues.Methods: Here, SPOP was overexpressed by lentiviral vector transfection in ACHN and Caki-1 cells, and SPOP was knocked down in Caki-2 cells with similar transfection methods. The transfection efficiency was evaluated by quantitative PCR and western blotting analyses. The role of SPOP in the proliferation, migration, invasion and apoptosis of cell lines was determined by the MTT, wound-healing, Transwell and flow cytometry assays. Moreover, the cells were treated with different drug concentrations in proliferation and apoptosis assays to investigate the effect of sunitinib and IFN-α2b on the proliferation and apoptosis of SPOP-overexpressing cells and SPOP-knockdown RCC cells. Finally, immunohistochemical staining of SPOP was performed in kidney tissues and TMAs, which included RCC tissues and corresponding adjacent normal tissues.Results: Overexpression of SPOP inhibited cell proliferation, migration and invasion and increased cell apoptosis. Interestingly, sunitinib and IFN-α2b at several concentrations increased the proliferation inhibitory rate and total apoptosis rate of cells overexpressing SPOP. The findings of the present study showed that the SPOP protein was significantly expressed at low levels in most clear cell RCC (ccRCC) tissues and at relatively high levels in the majority of adjacent normal tissues and kidney tissues. Kaplan-Meier survival analysis showed that there was no statistically significant difference in cumulative survival based on the data of different SPOP expression levels in TMA and patients.Conclusions: In contrast to previous studies, our findings demonstrated that overexpression of SPOP might suppress the progression of RCC cells, which was supported by cell experiments and immunohistochemical staining. SPOP could be a potential tumour inhibitor in RCC.

scholarly journals The Role of Mitochondrial DNA Copy Number in Mammalian Fertility1

2010 ◽  
Vol 83 (1) ◽  
pp. 52-62 ◽  
Author(s):  
Timothy Wai ◽  
Asangla Ao ◽  
Xiaoyun Zhang ◽  
Daniel Cyr ◽  
Daniel Dufort ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Copy Number ◽  
Dna Copy Number ◽  
Mitochondrial Dna Copy Number

Critical role of TRPC6 channels in the development of human renal cell carcinoma

2013 ◽  
Vol 40 (8) ◽  
pp. 5115-5122 ◽  
Author(s):  
Jiayin Song ◽  
Yong Wang ◽  
Xue Li ◽  
Yanna Shen ◽  
Meilin Yin ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Renal Cell ◽  
Critical Role ◽  
Human Renal Cell Carcinoma
Sign in / Sign up

Export Citation Format

Share Document