Signal transmission at invaginating cone photoreceptor synaptic contacts following deletion of the presynaptic cytomatrix protein Bassoon in mouse retina

2019 ◽  
Vol 226 (2) ◽  
pp. e13241 ◽  
Author(s):  
Norbert Babai ◽  
Kaspar Gierke ◽  
Tanja Müller ◽  
Hanna Regus‐Leidig ◽  
Johann H. Brandstätter ◽  
...  
Keyword(s):  
Signal Transmission ◽  
Mouse Retina ◽  
Cone Photoreceptor ◽  
Synaptic Contacts

scholarly journals A QTL on Chromosome 10 Modulates Cone Photoreceptor Number in the Mouse Retina

2011 ◽  
Vol 52 (6) ◽  
pp. 3228 ◽  
Author(s):  
Irene E. Whitney ◽  
Mary A. Raven ◽  
Lu Lu ◽  
Robert W. Williams ◽  
Benjamin E. Reese
Keyword(s):  
Mouse Retina ◽  
Cone Photoreceptor ◽  
Chromosome 10

scholarly journals Local signals in mouse horizontal cell dendrites

2017 ◽  
Author(s):  
Camille A. Chapot ◽  
Christian Behrens ◽  
Luke E. Rogerson ◽  
Tom Baden ◽  
Sinziana Pop ◽  
...  
Keyword(s):  
Glutamate Release ◽  
Horizontal Cell ◽  
Horizontal Cells ◽  
Single Type ◽  
Mouse Retina ◽  
Gabaergic Interneuron ◽  
List Type ◽  
Cone Photoreceptor ◽  
Cone Photoreceptors ◽  
Temporal Properties

SummaryThe mouse retina contains a single type of horizontal cell, a GABAergic interneuron that samples from all cone photoreceptors within reach and modulates their glutamatergic output via parallel feedback mechanisms. Because horizontal cells form an electrically-coupled network, they have been implicated in global signal processing, such as large scale contrast enhancement. Recently, it has been proposed that horizontal cells can also act locally at the level of individual cone photoreceptors. To test this possibility physiologically, we used two-photon microscopy to record light stimulus-evoked Ca2+signals in cone axon terminals and horizontal cell dendrites as well as glutamate release in the outer plexiform layer. By selectively stimulating the two mouse cone opsins with green and UV light, we assessed whether signals from individual cones remain “isolated” within horizontal cell dendritic tips, or whether they spread across the dendritic arbour. Consistent with the mouse‘s opsin expression gradient, we found that the Ca2+signals recorded from dendrites of dorsal horizontal cells were dominated by M- and those of ventral horizontal cells by S-opsin activation. The signals measured in neighbouring horizontal cell dendritic tips varied markedly in their chromatic preference, arguing against global processing. Rather, our experimental data and results from biophysically realistic modelling support the idea that horizontal cells can process cone input locally, extending the “classical” view of horizontal cells function. Pharmacologically removing horizontal cells from the circuitry reduced the sensitivity of the cone signal to low frequencies, suggesting that local horizontal cell feedback shapes the temporal properties of cone output.HighlightsLight-evoked Ca2+signals in horizontal cell dendrites reflect opsin gradientChromatic preferences in neighbouring dendritic tips vary markedlyMouse horizontal cells process cone photoreceptor input locallyLocal horizontal cell feedback shapes the temporal properties of cone outputeTOC BlurbChapot et al. show that local light responses in mouse horizontal cell dendrites inherit properties, including chromatic preference, from the presynaptic cone photoreceptor, suggesting that their dendrites can provide “private” feedback to cones, for instance, to shape the temporal filtering properties of the cone synapse.

scholarly journals Pou2f1 and Pou2f2 cooperate to control the timing of cone photoreceptor production in the developing mouse retina

Development ◽  
2020 ◽  
Vol 147 (18) ◽  
pp. dev188730 ◽  
Author(s):  
Awais Javed ◽  
Pierre Mattar ◽  
Suying Lu ◽  
Kamil Kruczek ◽  
Magdalena Kloc ◽  
...  
Keyword(s):  
Cell Types ◽  
Mouse Retina ◽  
Cone Photoreceptor ◽  
Chronological Order ◽  
Temporal Window ◽  
General Role ◽  
Cone Production ◽  
Regulatory Cascade ◽  
The People ◽  
Temporal Identity

ABSTRACTMultipotent retinal progenitor cells (RPCs) generate various cell types in a precise chronological order, but how exactly cone photoreceptor production is restricted to early stages remains unclear. Here, we show that the POU-homeodomain factors Pou2f1/Pou2f2, the homologs of Drosophila temporal identity factors nub/pdm2, regulate the timely production of cones in mice. Forcing sustained expression of Pou2f1 or Pou2f2 in RPCs expands the period of cone production, whereas misexpression in late-stage RPCs triggers ectopic cone production at the expense of late-born fates. Mechanistically, we report that Pou2f1 induces Pou2f2 expression, which binds to a POU motif in the promoter of the rod-inducing factor Nrl to repress its expression. Conversely, conditional inactivation of Pou2f2 in RPCs increases Nrl expression and reduces cone production. Finally, we provide evidence that Pou2f1 is part of a cross-regulatory cascade with the other temporal identity factors Ikzf1 and Casz1. These results uncover Pou2f1/2 as regulators of the temporal window for cone genesis and, given their widespread expression in the nervous system, raise the possibility of a general role in temporal patterning.This article has an associated ‘The people behind the papers’ interview.

Immunohistochemical analysis of the outer plexiform layer in the nob mouse shows no abnormalities

2003 ◽  
Vol 20 (3) ◽  
pp. 267-272 ◽  
Author(s):  
SHERRY L. BALL ◽  
MACHELLE T. PARDUE ◽  
MAUREEN A. MCCALL ◽  
RONALD G. GREGG ◽  
NEAL S. PEACHEY
Keyword(s):  
Expression Pattern ◽  
Signal Transmission ◽  
Plexiform Layer ◽  
Immunohistochemical Analysis ◽  
Bipolar Cells ◽  
Outer Plexiform Layer ◽  
Mouse Retina ◽  
Voltage Gated ◽  
Voltage Gated Calcium Channels ◽  
Immunohistochemical Techniques

In the nob mouse, a mutation in nyctalopin results in a loss of signal transmission from photoreceptors to depolarizing bipolar cells (DBCs). We used immunohistochemical techniques to assess the expression pattern of proteins found at either the photoreceptor terminal or bipolar cell dendrites within the outer plexiform layer. We labeled normal and nob retinas with antibodies against mGluR6, PKC, G0α, bassoon, PSD-95, the α1F subunit of voltage-gated calcium channels, trkB, and dystrophin. All labeling patterns in nob and normal retinas were comparable to those previously reported in mouse retina. Our results indicate that the absence of nyctalopin does not disrupt the expression pattern of other proteins known to be required for synaptic transmission.

scholarly journals Light-mediated planar polarization of cone photoreceptor cilia contributes to visual acuity in mammals

2021 ◽  
Author(s):  
Michael Housset ◽  
Dominic Filion ◽  
Nelson Cortes ◽  
Hojatollah Vali ◽  
Craig Mandato ◽  
...  
Keyword(s):  
Visual Acuity ◽  
Cell Polarity ◽  
Planar Cell Polarity ◽  
Mouse Retina ◽  
G Protein Signaling ◽  
Protein Signaling ◽  
Cone Photoreceptor ◽  
Critical Window ◽  
Mammalian Retina ◽  
Fly Eye

Planar cell polarity (PCP) is essential to optimize information processing and functional response in many tissues. While the fly eye is a classic example of PCP, it remains unknown whether PCP exists in the mammalian retina and whether it plays a part in vision. Here we used 3D reconstructions of the mouse retina to show that the basal body of cone photoreceptor cilia, but not rods, is systematically located on the side of the cell facing the centre of the retina. We further show that light is required during a critical window of development to establish cone PCP, and that both cone transducin and the G-protein signaling modulator protein 2 are required to mediate this effect. Importantly, we report that disruption of cone PCP impairs visual acuity. This work uncovers a non-canonical PCP pathway, mediated by light, and identifies cone PCP as a feature supporting mammalian vision.

cGMP-dependent cone photoreceptor degeneration in the cpfl1 mouse retina

2010 ◽  
Vol 518 (17) ◽  
pp. 3604-3617 ◽  
Author(s):  
Dragana Trifunović ◽  
Katja Dengler ◽  
Stylianos Michalakis ◽  
Eberhart Zrenner ◽  
Bernd Wissinger ◽  
...  
Keyword(s):  
Mouse Retina ◽  
Cone Photoreceptor ◽  
Photoreceptor Degeneration

scholarly journals Recoverin Improves Rod-Mediated Vision by Enhancing Signal Transmission in the Mouse Retina

Neuron ◽  
2005 ◽  
Vol 46 (3) ◽  
pp. 413-420 ◽  
Author(s):  
Alapakkam P. Sampath ◽  
Katherine J. Strissel ◽  
Rajesh Elias ◽  
Vadim Y. Arshavsky ◽  
James F. McGinnis ◽  
...  
Keyword(s):  
Signal Transmission ◽  
Mouse Retina
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