Extracellular adenosine initiates rapid arteriolar vasodilation induced by a single skeletal muscle contraction in hamster cremaster muscle

2013 ◽  
Vol 208 (1) ◽  
pp. 74-87 ◽  
Author(s):  
G. A. Ross ◽  
M. L. Mihok ◽  
C. L. Murrant
Keyword(s):  
Skeletal Muscle ◽  
Muscle Contraction ◽  
Cremaster Muscle ◽  
Extracellular Adenosine ◽  
Skeletal Muscle Contraction ◽  
Arteriolar Vasodilation

Skeletal muscle contraction-induced vasodilator complement production is dependent on stimulus and contraction frequency

2009 ◽  
Vol 297 (1) ◽  
pp. H433-H442 ◽  
Author(s):  
Ashok K. Dua ◽  
Nickesh Dua ◽  
Coral L. Murrant
Keyword(s):  
Nitric Oxide ◽  
Skeletal Muscle ◽  
Muscle Contraction ◽  
Muscle Fibers ◽  
Nitric Oxide Donor ◽  
Contraction Frequency ◽  
Skeletal Muscle Contraction ◽  
Train Duration ◽  
Arteriolar Vasodilation ◽  
40 Hz

To test the hypothesis that the vasodilator complement that produces arteriolar vasodilation during muscle contraction depends on both stimulus and contraction frequency, we stimulated four to five skeletal muscle fibers in the anesthetized hamster cremaster preparation in situ and measured the change in diameter of arterioles at a site of overlap with the stimulated muscle fibers. Diameter was measured before, during, and after 2 min of skeletal muscle contraction stimulated over a range of stimulus frequencies [4, 20, and 40 Hz; 15 contractions/min (cpm), 250 ms train duration] and a range of contraction frequencies (6, 15, and 60 cpm; 20 Hz stimulus frequency, 250 ms train duration). Muscle fibers were stimulated in the absence and presence of an inhibitor of adenosine receptors [10−6 M xanthine amine congener (XAC)], an ATP-dependent potassium (K+) channel inhibitor (10−5 M glibenclamide), an inhibitor of a source of K+ by inhibition of voltage-dependent K+ channels [3 × 10−4 M 3,4-diaminopyridine (DAP)], and an inhibitor of nitric oxide synthase [10−6 M NG-nitro-l-arginine methyl ester (l-NAME) + 10−7 S-nitroso- N-acetylpenicillamine (a nitric oxide donor)]. l-NAME inhibited the dilations at all stimulus frequencies and contraction frequencies except 60 cpm. XAC inhibited the dilations at all contraction frequencies and stimulus frequencies except 40 Hz. Glibenclamide inhibited all dilations at all stimulus and contraction frequencies, and DAP did not inhibit dilations at any stimulus frequencies while attenuating dilation at a contraction frequency of 60 cpm only. Our data show that the complement of dilators responsible for the vasodilations induced by skeletal muscle contraction differed depending on the stimulus and contraction frequency; therefore, both are important determinants of the dilators involved in the processes of arteriolar vasodilation associated with active hyperemia.

Local and remote arteriolar dilations initiated by skeletal muscle contraction

2000 ◽  
Vol 279 (5) ◽  
pp. H2285-H2294 ◽  
Author(s):  
Coral L. Murrant ◽  
Ingrid H. Sarelius
Keyword(s):  
Skeletal Muscle ◽  
Muscle Contraction ◽  
Muscle Fibers ◽  
Muscle Metabolism ◽  
Cremaster Muscle ◽  
Remote Site ◽  
Upstream Site ◽  
Skeletal Muscle Contraction ◽  
Perivascular Nerves ◽  
The Relationship

To investigate the relationship between skeletal muscle metabolism and arteriolar dilations in the region local to contracting muscle fibers as well as dilations at remote arteriolar regions upstream, we used a microelectrode on cremaster muscle of anesthetized hamsters to stimulate four to five muscle fibers lying approximately perpendicular to and overlapping a transverse arteriole. Before, during, and after muscle contraction, we measured the diameter of the arteriole at the site of muscle fiber overlap (local) and at a remote site ∼1,000 μm upstream. Two minutes of 2-, 4-, or 8-Hz stimulation (5–10 V, 0.4-ms duration) produced a significant dilation locally (8.2 ± 2.0-, 22.5 ± 2.4-, and 30.9 ± 2.1-μm increase, respectively) and at the remote site (4.2 ± 0.8, 11.0 ± 1.1, and 18.9 ± 2.7 μm, respectively). Muscle contraction at 4 Hz initiated a remote dilation that was unaffected by 15-min micropipette application of either 2 μM tetrodotoxin, 0.07% halothane, or 40 μM 18-β-glycyrrhetinic acid between the local and upstream site. Therefore, at the arteriolar level, muscle contraction initiates a robust remote dilation that does not appear to be transmitted via perivascular nerves or gap junctions.

Thermodynamic and Mechanical Properties of Skeletal Muscle Contraction

2005 ◽  
Vol 26 (3) ◽  
pp. 827-835 ◽  
Author(s):  
Yu. I. Prylutskyy ◽  
A. M. Shut ◽  
M. S. Miroshnychenko ◽  
A. D. Suprun
Keyword(s):  
Mechanical Properties ◽  
Skeletal Muscle ◽  
Muscle Contraction ◽  
Skeletal Muscle Contraction
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