China urgently needs a nationwide scoliosis screening system

2020 ◽  
Vol 109 (11) ◽  
pp. 2416-2417 ◽  
Author(s):  
Bin Yan ◽  
Xinhai Lu ◽  
Guohui Nie ◽  
Yeen Huang
Keyword(s):  
Screening System ◽  
Scoliosis Screening

Mental health screening system using intranet setting in a company.

1999 ◽  
Author(s):  
Minoru Arai ◽  
Daisuke Mori ◽  
Tetsu Kawamura ◽  
Hideo Fumimoto ◽  
Masagi Shimazaki ◽  
...  
Keyword(s):  
Mental Health ◽  
Health Screening ◽  
Mental Health Screening ◽  
Screening System ◽  
A Company

Glucagon induziert eine signifikante nukleäre Translokation von FOXO1, nachgewiesen mit einem neu entwickelten, hochsensitiven, GFP-FOXO1 basierten screening System

2010 ◽  
Vol 5 (S 01) ◽  
Author(s):  
C Bumke-Vogt ◽  
A Arafat ◽  
M Osterhoff ◽  
H Elkatry ◽  
A Ziegenhorn ◽  
...  
Keyword(s):  
Screening System

The Design Concept of the Saving Energy for OCC Screening System

2005 ◽  
Vol 59 (6) ◽  
pp. 819-824
Author(s):  
Toshio Dousaka ◽  
Naoyuki Iwashige
Keyword(s):  
Design Concept ◽  
Screening System ◽  
Saving Energy

A Web-Based Screening System For Dyslexic Pupils: Do Teachers Need It?

2012 ◽  
Vol 5 (4) ◽  
pp. 15-23
Author(s):  
Nor Hasbiah Bt. Ubaidullah ◽  
◽  
Jamilah Hamid ◽  
Keyword(s):  
Screening System ◽  
Web Based

scholarly journals Lentiviral CRISPR-guided RNA library screening identified Adam17 as an upstream negative regulator of Procr in mammary epithelium

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Ting Wu ◽  
Yinghua Wang ◽  
Tianxiong Xiao ◽  
Yirui Ai ◽  
Jinsong Li ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Membrane Proteins ◽  
Epithelial Cells ◽  
Adult Stem Cells ◽  
Vascular System ◽  
Negative Regulator ◽  
Breast Cancer Cell Lines ◽  
Screening System ◽  
Membrane Expression ◽  
Sorting Technique

Abstract Background Protein C receptor (Procr) has recently been shown to mark resident adult stem cells in the mammary gland, vascular system, and pancreatic islets. More so, high Procr expression was also detected and used as indicator for subsets of triple-negative breast cancers (TNBCs). Previous study has revealed Procr as a target of Wnt/β-catenin signaling; however, direct upstream regulatory mechanism of Procr remains unknown. To comprehend the molecular role of Procr during physiology and pathology, elucidating the upstream effectors of Procr is necessary. Here, we provide a system for screening negative regulators of Procr, which could be adapted for broad molecular analysis on membrane proteins. Results We established a screening system which combines CRISPR-Cas9 guided gene disruption with fluorescence activated cell sorting technique (FACS). CommaDβ (murine epithelial cells line) was used for the initial Procr upstream effector screening using lentiviral CRISPR-gRNA library. Shortlisted genes were further validated through individual lentiviral gRNA infection followed by Procr expression evaluation. Adam17 was identified as a specific negative inhibitor of Procr expression. In addition, MDA-MB-231 cells and Hs578T cells (human breast cancer cell lines) were used to verify the conserved regulation of ADAM17 over PROCR expression. Conclusion We established an efficient CRISPR-Cas9/FACS screening system, which identifies the regulators of membrane proteins. Through this system, we identified Adam17 as the negative regulator of Procr membrane expression both in mammary epithelial cells and breast cancer cells.

scholarly journals A High-Throughput Screening System Based on Fluorescence-Activated Cell Sorting for the Directed Evolution of Chitinase A

2021 ◽  
Vol 22 (6) ◽  
pp. 3041
Author(s):  
Gheorghita Menghiu ◽  
Vasile Ostafe ◽  
Radivoje Prodanović ◽  
Rainer Fischer ◽  
Raluca Ostafe
Keyword(s):  
High Throughput ◽  
Cell Sorting ◽  
High Throughput Screening ◽  
Screening Method ◽  
Enzymatic Reaction ◽  
Hydrolytic Enzymes ◽  
Screening System ◽  
Fluorescence Activated Cell Sorting ◽  
Fungal Cell ◽  
Chitinase A

Chitinases catalyze the degradation of chitin, a polymer of N-acetylglucosamine found in crustacean shells, insect cuticles, and fungal cell walls. There is great interest in the development of improved chitinases to address the environmental burden of chitin waste from the food processing industry as well as the potential medical, agricultural, and industrial uses of partially deacetylated chitin (chitosan) and its products (chito-oligosaccharides). The depolymerization of chitin can be achieved using chemical and physical treatments, but an enzymatic process would be more environmentally friendly and more sustainable. However, chitinases are slow-acting enzymes, limiting their biotechnological exploitation, although this can be overcome by molecular evolution approaches to enhance the features required for specific applications. The two main goals of this study were the development of a high-throughput screening system for chitinase activity (which could be extrapolated to other hydrolytic enzymes), and the deployment of this new method to select improved chitinase variants. We therefore cloned and expressed the Bacillus licheniformis DSM8785 chitinase A (chiA) gene in Escherichia coli BL21 (DE3) cells and generated a mutant library by error-prone PCR. We then developed a screening method based on fluorescence-activated cell sorting (FACS) using the model substrate 4-methylumbelliferyl β-d-N,N′,N″-triacetyl chitotrioside to identify improved enzymes. We prevented cross-talk between emulsion compartments caused by the hydrophobicity of 4-methylumbelliferone, the fluorescent product of the enzymatic reaction, by incorporating cyclodextrins into the aqueous phases. We also addressed the toxicity of long-term chiA expression in E. coli by limiting the reaction time. We identified 12 mutants containing 2–8 mutations per gene resulting in up to twofold higher activity than wild-type ChiA.

scholarly journals Application of Psychometric Approach for ASD Evaluation in Russian 3–4-Year-Olds

Mathematics ◽  
2021 ◽  
Vol 9 (14) ◽  
pp. 1608
Author(s):  
Andrey Nasledov ◽  
Sergey Miroshnikov ◽  
Liubov Tkacheva ◽  
Kirill Miroshnik ◽  
Meriam Uld Semeta
Keyword(s):  
Autistic Spectrum Disorder ◽  
Online Survey ◽  
Total Sample ◽  
Compact Set ◽  
Healthy Controls ◽  
Screening System ◽  
Children With Asd ◽  
Discriminant Analyses ◽  
Biological Problem ◽  
Choice Tasks

Background: Autistic spectrum disorder (ASD) is a significant socio-biological problem due to its wide prevalence and negative outcomes. In the current study, we aimed to develop an autism scale for early and accurate differentiation of 3- to 4-year-olds at risk for ASD since there is no systematic monitoring of young children in Russia yet. Methods: The total sample (N = 324) included 116 children with ASD, 131 children without ASD (healthy controls), and 77 children with developmental delay (DD). An online survey of specialists working with children was conducted based on a specially designed autism questionnaire consisting of 85 multiple-choice tasks distributed across 12 domains. Initially, each child was assessed by 434 items using a dichotomous scale (0 = no, 1 = yes). Factor and discriminant analyses were performed to identify a compact set of subscales that most accurately and with sufficient reliability predicted whether a child belongs to the ASD group. Results: As a result, four subscales were obtained: Sensorics, Emotions, Hyperactivity, and Communication. The high discriminability of the subscales in distinguishing the ASD group from the non-ASD group was revealed (accuracy 85.5–87.0%). Overall, the obtained subscales meet psychometric requirements and allow for creating an online screening system for wide application.

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