scholarly journals DSAEK ‐derived glider technique to introduce human amniotic membrane patch through small‐gauge trocar for retinal pathologies

2019 ◽  
Vol 98 (4) ◽  
Author(s):  
Tomaso Caporossi ◽  
Ruggero Tartaro ◽  
Bianca Pacini ◽  
Lorenzo De Angelis ◽  
Stanislao Rizzo
Keyword(s):  
Amniotic Membrane ◽  
Human Amniotic Membrane ◽  
Small Gauge ◽  
Retinal Pathologies ◽  
Membrane Patch

Human amniotic membrane for failed macular hole. A case of initial unsuccessful outcome that resolved after amniotic membrane exchange

2021 ◽  
pp. 112067212110589
Author(s):  
Tomaso Caporossi ◽  
Lorenzo Governatori ◽  
Tommaso Verdina ◽  
Stanislao Rizzo
Keyword(s):  
Visual Acuity ◽  
Macular Hole ◽  
Amniotic Membrane ◽  
Sulfur Hexafluoride ◽  
Silicone Oil ◽  
Human Amniotic Membrane ◽  
Second Surgery ◽  
Myopic Macular Hole ◽  
Oil Injection ◽  
Membrane Patch

Introduction We described a case of initial unsuccessful outcome of failed macular hole treated with amniotic membrane, that resolved after amniotic membrane exchange and silicone oil injection. Case Description a woman affected by a high myopic macular hole that had failed to close after multiple surgeries, had been treated using an amniotic membrane graft and 20% sulfur hexafluoride but, after the gas reabsorption, the macular hole was still open, and the amniotic membrane. A second surgery with a second amniotic membrane patch and silicone oil was carried out. Two weeks after the procedure the macular hole closed, and the final visual acuity improved from 20/400 to 20/100. Conclusions In this case, a second surgery, using a new amniotic membrane graft and a longer-lasting endotamponade, closed the hole and improved the visual acuity.

Management of Optic Disk Pit-associated Macular Detachment with Human Amniotic Membrane Patch

Retina ◽  
2020 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Stanislao Rizzo ◽  
Tomaso Caporossi ◽  
Bianca Pacini ◽  
Lorenzo De Angelis ◽  
Maria Luce De Vitto ◽  
...  
Keyword(s):  
Amniotic Membrane ◽  
Optic Disk ◽  
Human Amniotic Membrane ◽  
Macular Detachment ◽  
Membrane Patch

scholarly journals Applications of the Amniotic Membrane in Vitreoretinal Surgery

2020 ◽  
Vol 9 (8) ◽  
pp. 2675
Author(s):  
Tomaso Caporossi ◽  
Ruggero Tartaro ◽  
Daniela Bacherini ◽  
Bianca Pacini ◽  
Lorenzo De Angelis ◽  
...  
Keyword(s):  
Retinal Detachment ◽  
Amniotic Membrane ◽  
Surgical Techniques ◽  
Vitreoretinal Surgery ◽  
Recovery Process ◽  
Age Related Macular Degeneration ◽  
Human Amniotic Membrane ◽  
Serous Macular Detachment ◽  
Age Related ◽  
Retinal Pathologies

Recently, the use of the human amniotic membrane (hAM) has been extended to treat retinal disorders, such as macular holes that failed to close and retinal tears. The hAM has demonstrated the induction of a recovery process of the external retinal layers involving the external limiting membrane (ELM) and the ellipsoid zone (EZ). After that, the application of the hAM for retinal pathologies was extended to large macular tears, high myopic retinal detachment associated with MH, paravascular tears, serous macular detachment associated with optic pit, complicated retinal detachment and advanced age-related macular degeneration (AMD). The hAM has shown a potential in repairing retinal tissue through a regeneration process. This review aims to highlight the use of the hAM in various vitreo-retinal surgical fields, and to confront it with other cutting-edge surgical techniques used to treat challenging vitreo-retinal pathologies.

Amniotic membrane for retinal detachment due to paravascular retinal breaks over patchy chorioretinal atrophy in pathologic myopia

2019 ◽  
Vol 30 (2) ◽  
pp. 392-395 ◽  
Author(s):  
Tomaso Caporossi ◽  
Lorenzo De Angelis ◽  
Bianca Pacini ◽  
Stanislao Rizzo
Keyword(s):  
Optical Coherence Tomography ◽  
Amniotic Membrane ◽  
Silicone Oil ◽  
Internal Limiting Membrane ◽  
Pathologic Myopia ◽  
Human Amniotic Membrane ◽  
Optical Coherence ◽  
Chorioretinal Atrophy ◽  
Retinal Breaks ◽  
Membrane Patch

Purpose: To describe a new surgical technique, using a human amniotic membrane patch, in two cases of retinal detachment with paravascular retinal breaks over patchy chorioretinal atrophy in pathologic myopia, already underwent pars plana vitrectomy with the internal limiting membrane peeling for myopic foveoschisis. Methods: Surgical technique description with surgical video. A 23-gauge pars plana vitrectomy was performed. A human amniotic membrane patch was implanted under the neuroretina to seal the posterior retinal break. Standard silicone oil tamponade was performed at the end of the surgery. The patients were positioned face down after the operation for the first week. Optical coherence tomography scans were used in the follow-ups. Results: The 2 weeks postoperative optical coherence tomography showed a new tissue growth over the human amniotic membrane patch. The 3 months optical coherence tomography showed the new tissue entirely covering the human amniotic membrane plug. Visual acuity improved from 20/2000 (2 LogMAR) to 20/250 (1.1 LogMAR) 3 months after the operation in both patients. The silicone oil was extracted 2 months after surgery, and no recurrences were observed. The patient’s visual acuity remained stable at 20/250 after the silicone oil extraction. Conclusion: In these complex cases, human amniotic membrane transplantation can be a valid option, when internal limiting membrane has already been peeled in previous surgeries, to seal the posterior retinal breaks over high myopic chorioretinal atrophy, with encouraging results.

scholarly journals Lyophilized amniotic membrane patch (LAMPatch) as a replacement of tamponades in the treatment of primary rhegmatogenous retinal detachment

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Mario Saravia ◽  
Luis Zeman ◽  
Alejandro Berra
Keyword(s):  
Intraocular Pressure ◽  
Retinal Detachment ◽  
Amniotic Membrane ◽  
Case Series ◽  
Pressure Rise ◽  
Human Amniotic Membrane ◽  
Retinal Breaks ◽  
23 Gauge ◽  
Membrane Patch

Abstract Background The basis of retinal detachment repair is sealing the retinal breaks. In order to seal the retinal breaks, chorioretinal adhesion around these lesions has to be achieved. Laser retinopexy is not immediate thus necessitates the use of a temporal endotamponade to maintain both tissues in apposition. We propose the use of a patch of lyophilized human amniotic membrane (LAMPatch) in order to occlude the retinal tear effectively until the chorioretinal adhesion is settled, overcoming the risks and limitations of the current tamponades. Methods 23-gauge vitrectomy was performed on eyes with primary retinal detachment with single retinal breaks of less than one-hour extension. A LAMPatch was deployed over the retinal breaks after retina was repositioned with perfluorocarbon. Neither gas nor silicon oil were injected. Results Six eyes of six patients with total or partial retinal detachment were included. Retinas remained reattached in all cases until the end on follow-up (3, 5 months). Best-corrected visual acuity at 1-week postop was between 20/30 and 20/100. Neither elevations of intraocular pressure, cataracts nor signs of inflammation were registered during follow-up. No second surgeries were needed. Conclusion This technique has proven to be safe and effective in this small case series. No intraocular pressure rise, inflammation or cataracts were registered until last follow-up visit.

Effect of viable cryopreserved human amniotic membrane sutured to cornea on course of experimental bacterial keratitis

2015 ◽  
Vol 57 (5) ◽  
pp. 35-41
Author(s):  
K. Sereda ◽  
◽  
G. Drozhzhina ◽  
T. Gaidamaka ◽  
V. Vit ◽  
...  
Keyword(s):  
Amniotic Membrane ◽  
Human Amniotic Membrane ◽  
Bacterial Keratitis

scholarly journals Human Amniotic Membrane as a Matrix for Endothelial Differentiation of VEGF-Treated Dental Stem Cells

2019 ◽  
Vol 12 (6) ◽  
pp. 599-613 ◽  
Author(s):  
Siti Nurnasihah Md Hashim ◽  
Muhammad Fuad Hilmi Yusof ◽  
Wafa’ Zahari ◽  
Hamshawagini Chandra ◽  
Khairul Bariah Ahmad Amin Noordin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Amniotic Membrane ◽  
Endothelial Differentiation ◽  
Human Amniotic Membrane ◽  
Dental Stem Cells

Human amniotic membrane as a delivery vehicle for stem cell-based therapies

Life Sciences ◽  
2021 ◽  
pp. 119157
Author(s):  
Ping Chen ◽  
Minjun Lu ◽  
Tao Wang ◽  
Dongchun Dian ◽  
Yong Zhong ◽  
...  
Keyword(s):  
Stem Cell ◽  
Amniotic Membrane ◽  
Human Amniotic Membrane ◽  
Delivery Vehicle

scholarly journals Anti-inflammatory properties of amniotic membrane patch following pericardiectomy for constrictive pericarditis

2017 ◽  
Vol 12 (1) ◽  
Author(s):  
Katherine M. Marsh ◽  
Alice S. Ferng ◽  
Tia Pilikian ◽  
Ankit A. Desai ◽  
Ryan Avery ◽  
...  
Keyword(s):  
Amniotic Membrane ◽  
Constrictive Pericarditis ◽  
Anti Inflammatory ◽  
Membrane Patch

scholarly journals The Antibacterial Activity of Human Amniotic Membrane against Multidrug-Resistant Bacteria Associated with Urinary Tract Infections: New Insights from Normal and Cancerous Urothelial Models

Biomedicines ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 218
Author(s):  
Taja Železnik Ramuta ◽  
Larisa Tratnjek ◽  
Aleksandar Janev ◽  
Katja Seme ◽  
Marjanca Starčič Erjavec ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Amniotic Membrane ◽  
Multidrug Resistant ◽  
Basic Knowledge ◽  
Resistant Bacteria ◽  
Human Amniotic Membrane ◽  
Multidrug Resistant Bacteria ◽  
Tract Infections

Urinary tract infections (UTIs) represent a serious global health issue, especially due to emerging multidrug-resistant UTI-causing bacteria. Recently, we showed that the human amniotic membrane (hAM) could be a candidate for treatments and prevention of UPEC and Staphylococcus aureus infections. However, its role against multidrug-resistant bacteria, namely methicillin-resistant S. aureus (MRSA), extended-spectrum beta-lactamases (ESBL) producing Escherichia coli and Klebsiella pneumoniae, vancomycin-resistant Enterococci (VRE), carbapenem-resistant Acinetobacter baumannii, and Pseudomonas aeruginosa has not yet been thoroughly explored. Here, we demonstrate for the first time that the hAM homogenate had antibacterial activity against 7 out of 11 tested multidrug-resistant strains, the greatest effect was on MRSA. Using novel approaches, its activity against MRSA was further evaluated in a complex microenvironment of normal and cancerous urinary bladder urothelia. Even short-term incubation in hAM homogenate significantly decreased the number of bacteria in MRSA-infected urothelial models, while it did not affect the viability, number, and ultrastructure of urothelial cells. The hAM patches had no antibacterial activity against any of the tested strains, which further exposes the importance of the hAM preparation. Our study substantially contributes to basic knowledge on the antibacterial activity of hAM and reveals its potential to be used as an antibacterial agent against multidrug-resistant bacteria.

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