Cell death pattern in lens epithelium of cataract patients

2016 ◽  
Vol 94 (5) ◽  
pp. 514-520 ◽  
Author(s):  
Øyvind Osnes-Ringen ◽  
Kristiane Haug Berg ◽  
Morten C. Moe ◽  
Charlotta Zetterström ◽  
Magnus Røger ◽  
...  
Keyword(s):  
Cell Death ◽  
Lens Epithelium ◽  
Cell Death Pattern ◽  
Cataract Patients

scholarly journals Distribution of starch, lipid and nuclei in xylem and phloem of Tectona grandis Linn.

2012 ◽  
Vol 19 ◽  
pp. 29-35 ◽  
Author(s):  
Md Azharul Islam ◽  
Shahanara Begum
Keyword(s):  
Cell Death ◽  
Tectona Grandis ◽  
Tree Breeding ◽  
Plant Tissues ◽  
Phloem Parenchyma ◽  
Tree Breeding Program ◽  
Teak Tree ◽  
Xylem Tissue ◽  
Staining Methods ◽  
Cell Death Pattern

Context: Reserve materials among different plant tissues vary species to species. The distribution pattern of such materials and cell death pattern in Tectona grandis Linn. are still obscure. Objectives: To study the localization of starch, lipid and nuclei in the phloem, cambium and xylem tissue of T. grandis. Materials and Methods: Blocks containing phloem, cambium and outermost xylem of the stem of 12 years old teak tree collected. Different staining methods used to visualize starch, lipid and nuclei within different cells under light microscope. Results: Starch in parenchyma cells is more abundant in outer xylem than phloem and cambium. Lipids droplets are uniformly distributed in outermost xylem. Phloem parenchyma content few mass of lipids but, limited in cambium. There are many dead cells visualized in both phloem and xylem with characteristic patterns. The results clarify the levels of starch, lipid in tissue of T. grandis and showed distinguished variation among the cell contents. Conclusion: The physiology of plant cells related to transport of nutrients and cell death also illustrated in the report. This would be helpful for further study to improve quality wood through tree breeding program. DOI: http://dx.doi.org/10.3329/jbs.v19i0.12997 J. bio-sci. 19: 29-35, 2011

scholarly journals Increased aquaporin 1 and 5 membrane expression in the lens epithelium of cataract patients

2016 ◽  
Vol 1862 (10) ◽  
pp. 2015-2021 ◽  
Author(s):  
Olatz Barandika ◽  
Maitane Ezquerra-Inchausti ◽  
Ander Anasagasti ◽  
Ainara Vallejo-Illarramendi ◽  
Irantzu Llarena ◽  
...  
Keyword(s):  
Lens Epithelium ◽  
Membrane Expression ◽  
Aquaporin 1 ◽  
Cataract Patients

scholarly journals Differences in Neuronal Numbers, Morphology and Developmental Apoptosis in Mice Nigra Provide Evidence of Ontogenic Origin of Vulnerability to Parkinson’s Disease

2021 ◽  
Author(s):  
D J Vidyadhara ◽  
Haorei Yarreiphang ◽  
Trichur R Raju ◽  
Phalguni Anand Alladi
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Cell Death ◽  
Neuronal Loss ◽  
Differential Susceptibility ◽  
Admixed Population ◽  
Resistant Strains ◽  
Dopaminergic Neuronal Loss ◽  
Cell Death Pattern ◽  
Developmental Cell Death

Abstract Parkinson’s disease (PD) prevalence varies by ethnicity. In an earlier study we replicated the reduced vulnerability to PD in an admixed population, using 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-susceptible C57BL/6J, MPTP-resistant CD-1 and their F1 crossbreds. In the present study we investigated if the differences have a developmental origin. Substantia nigra was evaluated atpostnatal days 2 (P2), P6, P10, P14, P18, and P22. C57BL/6J mice had smaller nigra and fewer dopaminergic neurons than the CD-1 and crossbreds at P2, which persisted through development. A significant increase in numbers and nigral volume was observed across strains till P14. A drastic decline thereafter was specific to C57BL/6J. CD-1 and crossbreds retained their numbers from P14 to stabilize with supernumerary neurons at adulthood. The neuronal size increased gradually to attain adult morphology at P10 in the resistant strains, vis-à-vis at P22 in C57BL/6J. Accordingly, in comparison to C57BL/6J, the nigra of CD-1 and reciprocal crossbreds possessed cyto-morphological features of resilience, since birth. The considerably lesser dopaminergic neuronal loss in the CD-1 and crossbreds seen at P2, P14 and thereafter was complemented by attenuated developmental cell death. The differences in programmed cell death were confirmed by reduced TUNEL labelling, AIF and caspase-3 expression. GDNF expression aligned with the cell death pattern at P2 and P14 in both nigra and striatum. Earlier maturity of nigra and its neurons appear to be better features that reflect as MPTP-resistance at adulthood. Thus variable MPTP-vulnerability in mice and also differential susceptibility to PD in humans may arise early during nigral development.

scholarly journals The effect of vaginal probiotic therapy on the outcome of papillomavirus infection

2022 ◽  
Vol 20 (4) ◽  
pp. 11-17
Author(s):  
V. V. Oleynik ◽  
E. A. Kremleva ◽  
A. V. Sgibnev
Keyword(s):  
Cell Death ◽  
Epithelial Cells ◽  
Hpv Infection ◽  
Vaginal Flora ◽  
Papillomavirus Infection ◽  
Vaginal Epithelial Cells ◽  
Vaginal Lactobacilli ◽  
Before And After ◽  
Infected Cells ◽  
Cell Death Pattern

Aim. To study the effect of vaginal probiotic therapy on the outcome of human papillomavirus (HPV) infection.Materials and methods. The study included HPV-infected patients: 29 patients with normal vaginal flora and 146 patients with a deficiency of vaginal lactobacilli, of which 117 patients received vaginal probiotic therapy. In samples obtained before and after the therapy, the effect of the probiotic on the change in the ratio of living, apoptotic, and necrotic vaginal epithelial cells after preliminary exposure to oxidative stress was studied.Results. It was found that probiotics reduce the number of infected epithelial cells that survived the oxidative damage and shift the balance of cell death forms towards apoptosis. Vaginal probiotic therapy in patients with a deficiency of lactobacilli increased the frequency of HPV elimination by 2.5 times and reduced the likelihood of treatment failure from 1.5 to 4 times, depending on the viral load. The probiotic therapy made the structure of HPV outcomes in Lactobacillus-deficient patients similar to that in patients with normal vaginal flora.Conclusion. Vaginal probiotic therapy improves outcomes of HPV infection in patients with a deficiency of lactobacilli by reducing the number of survived infected cells and shifting the cell death pattern towards apoptosis. 

scholarly journals Structural Characteristics of the Lens in Presenile Cataract

2021 ◽  
Vol 8 ◽  
Author(s):  
Sofija Andjelic ◽  
Kazimir Drašlar ◽  
Anastazija Hvala ◽  
Marko Hawlina
Keyword(s):  
Electron Microscopy ◽  
Epithelial Cells ◽  
Structural Characteristics ◽  
Structural Features ◽  
Lens Epithelium ◽  
Lens Epithelial Cells ◽  
Cortical Cataract ◽  
Apical Side ◽  
Cataract Patients ◽  
Presenile Cataract

The purpose of this work is to examine the structure of the anterior lens epithelial cells (aLECs) of presenile idiopathic cortical cataract to investigate the possible structural reasons for its development. The anterior lens capsules (aLCs: basement membrane and associated lens epithelial cells) were obtained from routine uneventful cataract surgery of 5 presenile cataract patients (16 and 41 years old women and 29, 39, and 45 years old men). None of the patients had family history of cataract, medication, or trauma and they were otherwise healthy. In addition, the patients did not have any other abnormal features in the ocular status except cataract. The aLCs were prepared for scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The most prominent abnormal features observed by SEM for all 5 studied presenile cataract patients were the changes of the aLECs structure with the dents, the selective concavity of some LECs, at their apical side centrally toward the nucleus. In addition, TEM showed the thinning of the lens epithelium with the segmentally concave cells and the compressed and elongated nuclei. Abnormal and distinguishable structural features were observed in the anterior lens epithelium aLECs in all 5 patients with presenile cataract. Disturbed structure of aLECs, regularly present in presenile cataract type is shown that might be associated with water accumulation in the presenile idiopathic cortical cataract lens.

Sign in / Sign up

Export Citation Format

Share Document