scholarly journals Effect of equilibration times, freezing, and thawing rates on post-thaw quality of buffalo (Bubalus bubalis) bull spermatozoa

Andrology ◽  
2016 ◽  
Vol 4 (5) ◽  
pp. 972-976 ◽  
Author(s):  
S. A. H. Shah ◽  
S. M. H. Andrabi ◽  
I. Z. Qureshi
Keyword(s):  
Bubalus Bubalis ◽  
Freezing And Thawing ◽  
Freezing And Thawing Rates

scholarly journals The Effect of Glutathione on The Quality of Aceh Local Catfish (Clarias gariepinus) Spermatozoa After Cryopreservation

2020 ◽  
Vol 12 (1) ◽  
pp. 125-131
Author(s):  
Raudhah Mahfudhah ◽  
Kartini Eriani ◽  
Zainal Abidin Muchlisin ◽  
Cut Ruhul Muthmainnah
Keyword(s):  
Plasma Membrane ◽  
Clarias Gariepinus ◽  
Membrane Integrity ◽  
Dna Integrity ◽  
Freezing And Thawing ◽  
Randomized Design ◽  
Fetal Bovine ◽  
Fresh Semen ◽  
Different Doses

The cryopreservation process might reduce the quality of spermatozoa due to an increase in the production of reactive oxygen species (ROS) compounds during cooling, freezing, and thawing. The quality of spermatozoa can be maintained by adding glutathione as an exogenous antioxidant into cryo-diluent. This study aimed to examine the effect of the addition of different doses of glutathione in cryopreservation of Aceh Local catfish (Clarias gariepinus) spermatozoa after freezing. The method used was a completely randomized design (CRD) with four treatments and four replications. Fresh semen was diluted in Ringer, 15% DMSO, and 20% Fetal Bovine Serum (FBS) and then added with glutathione antioxidants of 0.0 mgL-1, 0.5 mgL-1, 1.0 mgL-1, and 2.0 mgL-1. The parameters observed in this study were motility, integrity of the plasma membrane, fertility, and DNA integrity. The results showed that the concentration of glutathione had no effect on motility, integrity ofthe plasma membrane, or fertility, but had an effect on DNA integrity. The highest percentage of motility and plasma membrane integrity respectively was 40.50% (P3) and 70.87% (P2). Furthermore, the assessment of DNA integrity showed that there was no DNA fragmentation both treatments and fresh spermatozoa. This research is the first study regarding glutathione supplementation in cryo-diluent of Aceh Local catfish spermatozoa. Finally, the results obtained can provide information about the exact concentration of glutathione in the extender on the quality of spermatozoa of Aceh Local catfish (C. gariepinus) after the cryopreservation process. These results can also increase the success of fertility be used by the seed hall unit and the aquaculture industry to increase the productivity and supply high quality seeds.

scholarly journals KUALITAS SPERMATOZOA ASAL CAPUT, CORPUS, DAN CAUDA EPIDIDIMIS PADA KERBAU RAWA (Bubalus bubalis carabenensis)

2016 ◽  
Vol 3 (3) ◽  
pp. 97
Author(s):  
Muhammad Riyadhi ◽  
Akbar Budiansa ◽  
Muhammad Rizal
Keyword(s):  
Artificial Insemination ◽  
Bubalus Bubalis ◽  
Spermatozoa Motility ◽  
Swamp Buffaloes ◽  
Swamp Buffalo

The purpose of this research was evaluate the quality of spermatozoa concentration in the caput, corpus and cauda of the swamp buffalo epididymis (Bubalus bubalis carabanensis).  Method of this research was to exploration to 13 epididymides of eight swamp buffaloes were obtained from Banjar and Banjarmasin slaughterhouses,evaluated the quality of spermatozoa in caput, corpus and cauda of epididymis.  Quality of collected-spermatozoa including spermatozoa motility, percentage of live spermatozoa, spermatozoa concentration and percentage of abnormality.  Result of this study showed that mean of each of caput spermatozoa motility, percentage of live spermatozoa, spermatozoa concentration and percentage of abnormality; 0%, 45.43% (31.87–72%), 189,62 x106 (40–480 x106) and 56.16 %(44.34–66.53%), corpus ;2.77% (1–9%), 58.73% (45.14 –76%), 152.31 x106 (45 – 345x106), and 47.61 %(23.92 – 60.15%), cauda;53.46% (20 – 70%), 74.32 % (56.68 – 83%), 1,459.62 x106 (825 – 2,340x106), and 34.60%(15.89 –50.04%). In conclusion, spermatozoaofcaudaepididymis could be used in artificial insemination program.Keywords: Spermatozoa, epididymis, swamp buffalo.

Effect of freezing and thawing rates on denaturation of proteins in aqueous solutions

2003 ◽  
Vol 82 (6) ◽  
pp. 684-690 ◽  
Author(s):  
Enhong Cao ◽  
Yahuei Chen ◽  
Zhanfeng Cui ◽  
Peter R. Foster
Keyword(s):  
Aqueous Solutions ◽  
Freezing And Thawing ◽  
Freezing And Thawing Rates ◽  
Denaturation Of Proteins

scholarly journals Effect of Pre-Blended Phosphates on the Freezing Quality Characteristics of Ground Woody Breast Meat Compared to Normal Meat

Animals ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1880
Author(s):  
Laura J. Garner ◽  
Lasheda Brooks ◽  
Lindsey F. Spencer ◽  
John Rehm ◽  
Jasmine Kataria ◽  
...  
Keyword(s):  
Meat Quality ◽  
Sodium Tripolyphosphate ◽  
Frozen Storage ◽  
Meat Products ◽  
Gel Strength ◽  
Quality Analysis ◽  
Plate Count ◽  
Freezing And Thawing ◽  
Frozen Ground

Woody breast (WB) myopathy affected meat has a tough texture, higher cook loss, and decreased water holding capacity (WHC), and thus lower consumer acceptability. The WB meat can be ground and further converted into further processed products or frozen, stored, and shipped to further processors. Freezing and thawing of ground WB meat may further affect the quality of WB meat products. Hence, research is required to determine the effect of pre-blended phosphates on the quality of ground WB meat as well as its cryoprotective effect during frozen storage. The objective of this experiment was to investigate the effect of pre-blended phosphate levels on meat quality in WB and normal breast (NB) fillets before and after freezing. NB fillets and severely affected WB fillets were procured from a local commercial processor. The meat was separated into various treatment groups according to the sodium tripolyphosphate (STPP) inclusion levels (0, 0.25, and 0.5% w/w). The meat was ground with respective phosphate treatments and subdivided into vacuum-sealed bags (n = 240; 1 kg each). Half of the bags (n = 120) from each treatment were taken for meat quality analysis, while the other bags were placed in a freezer (−18 °C) for 6 days. Fresh samples were analyzed within 6–8 h while the frozen samples were thawed for 18 h at 4°C prior to analysis. Samples (n = 10) were analyzed for gel strength, pH, color (L* a* b*), proximate composition, and randomly selected samples (n = 5) were analyzed for aerobic plate count (APC). Experiments were repeated in two separate replications and the data was analyzed using the Proc Glimmix model procedure in SAS (v. 9.4) (Cary, NC, USA) with LSMeans Separation at p ≤ 0.05. The gel strength (g) of the fresh ground NB meat (883.7 g) was higher than the gel strength of woody meat (720.8 g) with 0% phosphate (p ≤ 0.05). Addition of phosphate (0.25 and 0.5%) significantly increased the gel strength of fresh woody meat but it was significantly lower than NB meat added with 0.25 and 0.5% phosphate treatment. After freezing, ground NB meat samples with 0.25 and 0.5% phosphate had higher gel strength compared to fresh and frozen ground WB meat (p ≤ 0.05). Pre-blended STPP raised the pH in all treatments (p < 0.05). Treatments did not have any clear impact on APC of ground WB or NB meat. Addition of pre-blended sodium tripolyphosphate increases the functionality of fresh and frozen ground WB meat, as well as NB meat.

scholarly journals A Study on the Properties of Recycled Aggregate Concrete and Its Production Facilities

2019 ◽  
Vol 9 (9) ◽  
pp. 1935 ◽  
Author(s):  
Jung-Ho Kim ◽  
Jong-Hyun Sung ◽  
Chan-Soo Jeon ◽  
Sae-Hyun Lee ◽  
Han-Soo Kim
Keyword(s):  
Drying Shrinkage ◽  
Quality Standards ◽  
Poor Quality ◽  
Recycled Aggregate Concrete ◽  
Recycled Aggregate ◽  
Freezing And Thawing ◽  
Construction Waste ◽  
Aggregate Concrete ◽  
Air Volume

In recent years, the amount of construction waste and recycled aggregate has been increasing every year in Korea. However, as the recycled aggregate is poor quality, it is not used for concrete, and the Korean government has strengthened the quality standards for recycled aggregate for concrete. In this study, research was conducted on the mechanical and durability characteristics of concrete using recycled aggregate, after developing equipment to improve the quality of recycled aggregate to increase the use of recycled aggregate for environmental improvements. The results illustrated improvements in the air volume, slump, compressive strength, freezing and thawing resistance, and drying shrinkage. Furthermore, this study is expected to contribute to the increased use of recycled aggregate in the future.

Effect of straw size and thawing time on quality of cryopreserved buffalo (Bubalus bubalis) semen

2011 ◽  
Vol 11 (1) ◽  
pp. 49-54 ◽  
Author(s):  
Muhammad S Ansari ◽  
Bushra A. Rakha ◽  
Syed M.H. Andrabi ◽  
Shamim Akhter
Keyword(s):  
Bubalus Bubalis

Effect of Antibiotics in Extender on Bacterial and Spermatozoal Quality of Cooled Buffalo (Bubalus bubalis ) Bull Semen

2007 ◽  
Vol 43 (3) ◽  
pp. 272-278 ◽  
Author(s):  
S Akhter ◽  
MS Ansari ◽  
SMH Andrabi ◽  
N Ullah ◽  
M Qayyum
Keyword(s):  
Bubalus Bubalis ◽  
Bull Semen
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