scholarly journals BTK inhibition is a potent approach to block IgE-mediated histamine release in human basophils

Allergy ◽  
2017 ◽  
Vol 72 (11) ◽  
pp. 1666-1676 ◽  
Author(s):  
D. Smiljkovic ◽  
K. Blatt ◽  
G. Stefanzl ◽  
Y. Dorofeeva ◽  
C. Skrabs ◽  
...  
Keyword(s):  
Histamine Release ◽  
Ige Mediated ◽  
Human Basophils

scholarly journals Enhancement of IgE-mediated histamine release from human basophils by viruses: role of interferon.

1977 ◽  
Vol 145 (4) ◽  
pp. 892-906 ◽  
Author(s):  
S Ida ◽  
J J Hooks ◽  
R P Siraganian ◽  
A L Notkins
Keyword(s):  
Tissue Culture ◽  
Antiviral Activity ◽  
Histamine Release ◽  
Influenza A ◽  
Culture Fluid ◽  
Soluble Factor ◽  
Release Histamine ◽  
Ige Mediated ◽  
Human Basophils

Human leukocytes maintained in culture are induced to release histamine when exposed to ragweed antigen E or anti-IgE. Leukocyte cultures incubated with virus (i.e. HSV-1, Influenza A, and Adeno-1) but not exposed to ragweed antigen E or anti-IgE fail to release histamine. If, however, leukocyte cultures are first exposed to virus and then to ragweed antigen E or anti-IgE, significant enhancement of histamine release occurs. Both infectious and inactivated virus enhance histamine release and the degree of enhancement is related to the concentration of virus and the length of the incubation. Tissue culture fluid harvested 8 h after exposure of leukocytes to virus contains a soluble factor which is capable of enhancing histamine release when added to fresh leukocyte cultures. This factor has all the properties of interferon including species specificity and cannot be dissociated from the antiviral activity of interferon. Moreover, both known inducers of interferon (poly I:poly C) and standard preparations of interferon are capable of enhancing histamine release. The enhancement of histamine release by interferon represents a new biological role for interferon.

Phosphatidylinositol-3 kinase regulates p21ras activation during IgE-mediated stimulation of human basophils

Blood ◽  
2000 ◽  
Vol 96 (6) ◽  
pp. 2199-2205 ◽  
Author(s):  
Katsushi Miura ◽  
Donald W. MacGlashan
Keyword(s):  
Histamine Release ◽  
Signaling Pathways ◽  
Cytosolic Calcium ◽  
Pi3 Kinase ◽  
Interleukin 4 ◽  
Regulatory Subunit ◽  
Functional Responses ◽  
Ige Antibody ◽  
Ige Mediated ◽  
Human Basophils

Abstract Cross-linking of IgE or a bacterial product (f-Met-Leu-Phe; FMLP) induces the release of leukotriene C4 (LTC4) and histamine in human basophils. However, the signaling mechanisms in human basophils are only partially understood. It has been demonstrated that extracellular signal-regulated kinases (ERK1/2) specifically regulate the pathway for LTC4 generation, but not for histamine release and interleukin-4 production. More recent studies have suggested that tyrosine kinase (syk)-mediated phosphorylation of shc is responsible for the ras-ERK cascade via the formation of shc-Grb2-Sos2 following stimulation with anti-IgE antibody, but not FMLP, in human basophils. However, while characterizing the role of phosphatidylinositol (PI)-3 kinase in signaling pathways leading to basophil mediator release, it was noted that this pathway might also regulate p21ras activation. Anti-IgE antibody, but not FMLP, resulted in phosphorylation of p85 (regulatory subunit of PI3 kinase), suggesting activation of PI3 kinase. Inhibition of PI3 kinase by selective inhibitor (LY294002) abolished anti-IgE antibody- but not FMLP-induced phosphorylation of MEK1 (MAPK kinase/ERK kinase) and ERKs while inhibiting LTC4 generation as well as histamine release. IgE-mediated activation of ras (upstream of MEK-ERK) was also inhibited. But, further upstream, phosphorylation of syk and of shc and inducible association between shc and Grb2 were not affected. Furthermore, the IgE-mediated cytosolic calcium response ([Ca++]i) was also diminished. These results suggest that functional responses may be dependent on the activity of PI3 kinase, which regulates at least 2 important signaling pathways: by regulating activation of ras for the MEK-ERK pathway and the increase in [Ca++]i.

Influence of Histamine H1, H2, and H3 Agonists and Antagonists on IgE Mediated Histamine Release from Human Basophils

1991 ◽  
pp. 152-157
Author(s):  
J. Kleine-Tebbe ◽  
J. Schramm ◽  
M. Bolz ◽  
H. Gagné ◽  
C. Josties ◽  
...  
Keyword(s):  
Histamine Release ◽  
Ige Mediated ◽  
Human Basophils

Inhibition of IgE-Mediated Histamine Release from Human Basophils and Mast Cells by Fenoterol

1984 ◽  
Vol 74 (4) ◽  
pp. 356-361 ◽  
Author(s):  
G. Marone ◽  
G. Ambrosio ◽  
D. Bonaduce ◽  
A. Genovese ◽  
M. Triggiani ◽  
...  
Keyword(s):  
Mast Cells ◽  
Histamine Release ◽  
Ige Mediated ◽  
Human Basophils
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