Inhibition of IgE-Mediated Histamine Release from Human Basophils and Mast Cells by Fenoterol

1984 ◽  
Vol 74 (4) ◽  
pp. 356-361 ◽  
Author(s):  
G. Marone ◽  
G. Ambrosio ◽  
D. Bonaduce ◽  
A. Genovese ◽  
M. Triggiani ◽  
...  
Keyword(s):  
Mast Cells ◽  
Histamine Release ◽  
Ige Mediated ◽  
Human Basophils

scholarly journals IgE-mediated anaphylactic degranulation of isolated human skin mast cells

Blood ◽  
1991 ◽  
Vol 77 (3) ◽  
pp. 569-578 ◽  
Author(s):  
AM Dvorak ◽  
W Massey ◽  
J Warner ◽  
S Kissell ◽  
A Kagey-Sobotka ◽  
...  
Keyword(s):  
Mast Cells ◽  
Histamine Release ◽  
Human Skin ◽  
Human Lung ◽  
Lipid Bodies ◽  
Fusion Event ◽  
Electron Microscopic ◽  
Ultrastructural Morphology ◽  
Ige Mediated

Isolated human skin mast cells (HSMC) were prepared and cultured overnight before functional and electron microscopic studies. Mast cell suspensions were examined after stimulation with anti-IgE to produce anaphylactic degranulation or examined in buffer-incubated controls. Histamine release was measured in replicate samples. Control, isolated HSMC studied by electron microscopy were well preserved and fully granulated. Although all granule patterns reported for human mast cells were found, crystal granules were the most prevalent, as is true for HSMC in situ. Individual mast cells containing both crystal and scroll granules occurred. Lipid bodies were rare, as in HSMC in situ. Control, isolated mast cells did not express granule changes associated with either piecemeal degranulation or recovery during wound healing in situ; nor were morphologic changes of anaphylactic degranulation present. Spontaneous histamine release was 0% in control samples. Anaphylactic degranulation of isolated HSMC was accompanied by 24% maximum histamine release and characteristically showed extrusion of altered, membrane-free granules through multiple pores in the plasma membrane to the exterior of the cell. Other morphologic aspects of anaphylactic degranulation, as expressed in isolated human lung mast cells, were also present. These events included granule swelling, fusion, alteration of matrix contents, degranulation channel formation, pore formation, and shedding of granules, membranes, and surface processes. The ultrastructural morphology of isolated HSMC and their IgE-mediated degranulation shows some differences from similar studies of isolated human lung mast cells and of human lung and gut mast cells in biopsy samples. These differences include crystal granules as the predominant granule pattern, minor numbers of lipid bodies, and extrusion of granules during anaphylactic degranulation as characteristic for HSMC. By contrast, isolated human lung and gut mast cells have more scroll granules and particle granules, respectively, and more lipid bodies. In isolated human lung mast cells, anaphylactic degranulation is almost exclusively an intracellular fusion event characterized by the formation of complex degranulation channels within which altered granule matrix materials solubilize. In addition to morphologic differences between mast cells of skin, lung, or gut origin, functional differences have also been reported among mast cells of these organs. The ultrastructural morphology of isolated HSMC is identical to that of skin mast cells in biopsy samples, thereby validating the usefulness of this new source of HSMC for correlative functional and morphologic studies.

scholarly journals Human C5a Induces a Substantial Histamine Release in Human Basophils but Not in Tissue Mast Cells

1988 ◽  
Vol 85 (4) ◽  
pp. 487-488 ◽  
Author(s):  
H. Jürgensen ◽  
U. Braam ◽  
P. Pult ◽  
E. Kownatzki ◽  
W. Schmutzler
Keyword(s):  
Mast Cells ◽  
Histamine Release ◽  
Human Basophils

Role of nitric oxide in histamine release from human basophils and rat peritoneal mast cells

2001 ◽  
Vol 425 (3) ◽  
pp. 229-238 ◽  
Author(s):  
Kheng H. Peh ◽  
Andrew Moulson ◽  
Beatrice Y.C. Wan ◽  
El-Sayed K. Assem ◽  
Frederick L. Pearce
Keyword(s):  
Nitric Oxide ◽  
Mast Cells ◽  
Histamine Release ◽  
Peritoneal Mast Cells ◽  
Rat Peritoneal Mast Cells ◽  
Human Basophils

scholarly journals IgE-mediated anaphylactic degranulation of isolated human skin mast cells

Blood ◽  
1991 ◽  
Vol 77 (3) ◽  
pp. 569-578 ◽  
Author(s):  
AM Dvorak ◽  
W Massey ◽  
J Warner ◽  
S Kissell ◽  
A Kagey-Sobotka ◽  
...  
Keyword(s):  
Mast Cells ◽  
Histamine Release ◽  
Human Skin ◽  
Human Lung ◽  
Lipid Bodies ◽  
Fusion Event ◽  
Electron Microscopic ◽  
Ultrastructural Morphology ◽  
Ige Mediated

Abstract Isolated human skin mast cells (HSMC) were prepared and cultured overnight before functional and electron microscopic studies. Mast cell suspensions were examined after stimulation with anti-IgE to produce anaphylactic degranulation or examined in buffer-incubated controls. Histamine release was measured in replicate samples. Control, isolated HSMC studied by electron microscopy were well preserved and fully granulated. Although all granule patterns reported for human mast cells were found, crystal granules were the most prevalent, as is true for HSMC in situ. Individual mast cells containing both crystal and scroll granules occurred. Lipid bodies were rare, as in HSMC in situ. Control, isolated mast cells did not express granule changes associated with either piecemeal degranulation or recovery during wound healing in situ; nor were morphologic changes of anaphylactic degranulation present. Spontaneous histamine release was 0% in control samples. Anaphylactic degranulation of isolated HSMC was accompanied by 24% maximum histamine release and characteristically showed extrusion of altered, membrane-free granules through multiple pores in the plasma membrane to the exterior of the cell. Other morphologic aspects of anaphylactic degranulation, as expressed in isolated human lung mast cells, were also present. These events included granule swelling, fusion, alteration of matrix contents, degranulation channel formation, pore formation, and shedding of granules, membranes, and surface processes. The ultrastructural morphology of isolated HSMC and their IgE-mediated degranulation shows some differences from similar studies of isolated human lung mast cells and of human lung and gut mast cells in biopsy samples. These differences include crystal granules as the predominant granule pattern, minor numbers of lipid bodies, and extrusion of granules during anaphylactic degranulation as characteristic for HSMC. By contrast, isolated human lung and gut mast cells have more scroll granules and particle granules, respectively, and more lipid bodies. In isolated human lung mast cells, anaphylactic degranulation is almost exclusively an intracellular fusion event characterized by the formation of complex degranulation channels within which altered granule matrix materials solubilize. In addition to morphologic differences between mast cells of skin, lung, or gut origin, functional differences have also been reported among mast cells of these organs. The ultrastructural morphology of isolated HSMC is identical to that of skin mast cells in biopsy samples, thereby validating the usefulness of this new source of HSMC for correlative functional and morphologic studies.

scholarly journals Enhancement of IgE-mediated histamine release from human basophils by viruses: role of interferon.

1977 ◽  
Vol 145 (4) ◽  
pp. 892-906 ◽  
Author(s):  
S Ida ◽  
J J Hooks ◽  
R P Siraganian ◽  
A L Notkins
Keyword(s):  
Tissue Culture ◽  
Antiviral Activity ◽  
Histamine Release ◽  
Influenza A ◽  
Culture Fluid ◽  
Soluble Factor ◽  
Release Histamine ◽  
Ige Mediated ◽  
Human Basophils

Human leukocytes maintained in culture are induced to release histamine when exposed to ragweed antigen E or anti-IgE. Leukocyte cultures incubated with virus (i.e. HSV-1, Influenza A, and Adeno-1) but not exposed to ragweed antigen E or anti-IgE fail to release histamine. If, however, leukocyte cultures are first exposed to virus and then to ragweed antigen E or anti-IgE, significant enhancement of histamine release occurs. Both infectious and inactivated virus enhance histamine release and the degree of enhancement is related to the concentration of virus and the length of the incubation. Tissue culture fluid harvested 8 h after exposure of leukocytes to virus contains a soluble factor which is capable of enhancing histamine release when added to fresh leukocyte cultures. This factor has all the properties of interferon including species specificity and cannot be dissociated from the antiviral activity of interferon. Moreover, both known inducers of interferon (poly I:poly C) and standard preparations of interferon are capable of enhancing histamine release. The enhancement of histamine release by interferon represents a new biological role for interferon.

Superallergens: a novel mechanism of IgE-mediated activation of human basophils and mast cells

2004 ◽  
Vol 4 (s2) ◽  
pp. 64-75 ◽  
Author(s):  
G. Marone ◽  
F. W. Rossi ◽  
M. Bova ◽  
A. Detoraki ◽  
B. Liccardo ◽  
...  
Keyword(s):  
Mast Cells ◽  
Ige Mediated ◽  
Human Basophils

Effects of Siegesbeckia pubescens on Immediate Hypersensitivity Reaction

1997 ◽  
Vol 25 (02) ◽  
pp. 163-167 ◽  
Author(s):  
Hyung Min Kim
Keyword(s):  
Mast Cells ◽  
Hypersensitivity Reaction ◽  
Histamine Release ◽  
Immunoglobulin E ◽  
Immediate Hypersensitivity ◽  
Antiallergic Activity ◽  
Peritoneal Mast Cells ◽  
Ige Mediated ◽  
Rat Peritoneal Mast Cells ◽  
Siegesbeckia Pubescens

This study was carried out to examine the effect of an aqueous extract from Siegesbeckia pubescens (Compositae) (SPAE) on immunoglobulin E (IgE)-mediated immediate hypersensitivity reaction. Forty-eight hours passive cutaneous anaphylaxis in rats was significantly inhibited by oral administration of SPAE (100 μg/g). It also inhibited histamine release from rat peritoneal mast cells induced by anti-dinitrophenyl (DNP)-IgE and DNP-human serum albumin. The data indicate that SPAE has antiallergic activity, and that its action may be due to inhibition of histamine release from mast cells.

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