Differences in cord blood extracellular vesicle cargo in preterm and term births

2022 ◽  
Author(s):  
Ramkumar Menon ◽  
Christopher Luke Dixon ◽  
Samir Cayne ◽  
Enkhtuya Radnaa ◽  
Carlos Salomon ◽  
...  
Keyword(s):  
Cord Blood ◽  
Extracellular Vesicle ◽  
Term Births

scholarly journals Cord Blood Extracellular Vesicles Analyzed by Flow Cytometry with Thresholding Using 405 nm or 488 nm Laser Leads to Concurrent Results

Diagnostics ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1320
Author(s):  
Kristýna Pekárková ◽  
Jakub Soukup ◽  
Marie Kostelanská ◽  
Jan Širc ◽  
Zbyněk Straňák ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Cord Blood ◽  
Extracellular Vesicles ◽  
Correlation Coefficients ◽  
Flow Cytometer ◽  
Liquid Biopsies ◽  
Physiological Conditions ◽  
Flow Cytometric ◽  
Term Births ◽  
And Control

Extracellular vesicles (EVs) from liquid biopsies are extensively analyzed by flow cytometry, a technology that is continuously evolving. Thresholding utilizing a violet 405 nm laser side scatter (VSSC) has recently been implemented. Here, we collected set of large EV (lEV) samples from cord blood, which we analyzed using a standard flow cytometer improved via a 405 nm laser side scatter. Samples were analyzed using two distinct thresholding methods—one based on VSSC, and one based on VSSC combined with fluorescence thresholding on stained phosphatidylserine. Through these thresholding methods, we compared lEVs from pre-term births and control cord blood. Double-labeled lEVs with platelet CD36+/CD41+, activated platelet CD41+/CD62P+ and endothelial CD31+/CD105+ antibodies were used. Apart from comparing the two groups together, we also correlated measured lEVs with the thresholding methods. We also correlated the results of this study with data analyzed in our previous study in which we used a conventional 488 nm laser SSC. We did not find any difference between the two cord blood groups. However, we found highly concurrent data via our correlation of the thresholding methods, with correlation coefficients ranging from 0.80 to 0.96 even though the numbers of detected lEVs differed between thresholding methods. In conclusion, our approaches to thresholding provided concurrent data and it seems that improving the cytometer with the use of a VSSC increases its sensitivity, despite not being particularly critical to the validity of flow cytometric studies that compare pathological and physiological conditions in liquid biopsies.

scholarly journals Umbilical cord tissue is a robust source for mesenchymal stem cells with enhanced myogenic differentiation potential compared to cord blood

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Shivangi Mishra ◽  
Jayesh Kumar Sevak ◽  
Anamica Das ◽  
G. Aneeshkumar Arimbasseri ◽  
Shinjini Bhatnagar ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Myogenic Differentiation ◽  
Differentiation Potential ◽  
Transcriptomic Sequencing ◽  
Differentiation Protocol ◽  
Umbilical Cord Tissue ◽  
Term Births

Abstract Differentiation of mesenchymal stem cells (MSCs) derived from two different sources of fetal tissues such as umbilical cord blood (UCB) and tissue (UCT) into skeletal muscle have remained underexplored. Here, we present a comparative analysis of UCB and UCT MSCs, in terms of surface markers, proliferation and senescence marker expression. We find that CD45−CD34− MSCs obtained from UCT and UCB of term births display differences in the combinatorial expression of key MSC markers CD105 and CD90. Importantly, UCT MSCs display greater yield, higher purity, shorter culture time, and lower rates of senescence in culture compared to UCB MSCs. Using a robust myogenic differentiation protocol, we show that UCT MSCs differentiate more robustly into muscle than UCB MSCs by transcriptomic sequencing and specific myogenic markers. Functional assays reveal that CD90, and not CD105 expression promotes myogenic differentiation in MSCs and could explain the enhanced myogenic potential of UCT MSCs. These results suggest that in comparison to large volumes of UCB that are routinely used to obtain MSCs and with limited success, UCT is a more reliable, robust, and convenient source of MSCs to derive cells of the myogenic lineage for both therapeutic purposes and increasing our understanding of developmental processes.

Abstract #91: Comparison of Cord Blood Insulin in Large-for-Gestational-Age Infants and Appropriate-for-Age Filipino Infants of Nondiabetic Mothers: A Pilot Study

2004 ◽  
Vol 10 ◽  
pp. 31
Author(s):  
Florence M. Amorado-Santos ◽  
Maria Honolina S. Gomez ◽  
Maria Victoria R. Olivares ◽  
Zayda N. Gamilla
Keyword(s):  
Pilot Study ◽  
Cord Blood ◽  
Gestational Age ◽  
Large For Gestational Age ◽  
Blood Insulin

Cord blood claims questioned

Nature ◽  
2003 ◽  
Author(s):  
Helen Pearson
Keyword(s):  
Cord Blood
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