scholarly journals Incomplete proline catabolism drives premature sperm aging

Aging Cell ◽  
2021 ◽  
Vol 20 (2) ◽  
Author(s):  
Chia‐An Yen ◽  
Sean P. Curran
Keyword(s):  
Proline Catabolism

scholarly journals Reactive Oxygen Species Homeostasis and Virulence of the Fungal PathogenCryptococcus neoformansRequires an Intact Proline Catabolism Pathway

Genetics ◽  
2013 ◽  
Vol 194 (2) ◽  
pp. 421-433 ◽  
Author(s):  
I. Russel Lee ◽  
Edmund Y. L. Lui ◽  
Eve W. L. Chow ◽  
Samantha D. M. Arras ◽  
Carl A. Morrow ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Proline Catabolism ◽  
Catabolism Pathway

scholarly journals Control of Expression of DivergentPseudomonas putida put Promoters for Proline Catabolism

2000 ◽  
Vol 66 (12) ◽  
pp. 5221-5225 ◽  
Author(s):  
Susana Vı́lchez ◽  
Maximino Manzanera ◽  
Juan L. Ramos
Keyword(s):  
Sequence Analysis ◽  
Transcription Initiation ◽  
Mrna Level ◽  
Regulatory Protein ◽  
Protein Sequence Analysis ◽  
Regulatory Activity ◽  
Proline Catabolism ◽  
C And N ◽  
Background Expression ◽  
Control Of Expression

ABSTRACT Pseudomonas putida KT2440 uses proline as the sole C and N source. Utilization of this amino acid involves its uptake, which is mediated by the PutP protein, and its conversion into glutamate, mediated by the PutA protein. Sequence analysis revealed that theputA and putP genes are transcribed divergently. Expression from the putP and putAgenes was analyzed at the mRNA level in different host backgrounds in the absence and presence of proline. Expression from theput promoters was induced by proline. The transcription initiation points of the putP and putA genes were precisely mapped via primer extension, and sequence analysis of the upstream DNA region showed well-separated promoters for these two genes. The PutA protein acts as a repressor of put gene expression in P. putida because expression from theput promoters is constitutive in a host background with a knockout putA gene. This regulatory activity is independent of the catabolic activity of PutA, because we show that a point mutation (Glu896→Lys) that prevents catalytic activity allowed the protein to retain its regulatory activity. Expression from theput promoters in the presence of proline in aputA-proficient background requires a positive regulatory protein, still unidentified, whose expression seems to be ς54 dependent because the put genes were not expressed in a ς54-deficient background. Expression of the putA and putP genes was equally high in the presence of proline in ς38- and ihf-deficientP. putida backgrounds.

scholarly journals Mitochondrial proline catabolism activates Ras1/cAMP/PKA-induced filamentation in Candida albicans

PLoS Genetics ◽  
2019 ◽  
Vol 15 (2) ◽  
pp. e1007976 ◽  
Author(s):  
Fitz Gerald S. Silao ◽  
Meliza Ward ◽  
Kicki Ryman ◽  
Axel Wallström ◽  
Björn Brindefalk ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Proline Catabolism

Molecular evolution and transcriptional regulation of the oilseed rape proline dehydrogenase genes suggest distinct roles of proline catabolism during development

Planta ◽  
2014 ◽  
Vol 241 (2) ◽  
pp. 403-419 ◽  
Author(s):  
Pascal Faës ◽  
Carole Deleu ◽  
Abdelkader Aïnouche ◽  
Françoise Le Cahérec ◽  
Emilie Montes ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Molecular Evolution ◽  
Oilseed Rape ◽  
Proline Dehydrogenase ◽  
Proline Catabolism

scholarly journals Put3 Positively Regulates Proline Utilization in Candida albicans

mSphere ◽  
2017 ◽  
Vol 2 (6) ◽  
Author(s):  
Walters Aji Tebung ◽  
Raha Parvizi Omran ◽  
Debra L. Fulton ◽  
Joachim Morschhäuser ◽  
Malcolm Whiteway
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Transcription Factor ◽  
Candida Albicans ◽  
Nitrogen Source ◽  
Opportunistic Pathogen ◽  
Baker's Yeast ◽  
Null Mutant ◽  
Multiple Sources ◽  
Content Type ◽  
Proline Catabolism

ABSTRACT Candida albicans poses a significant threat to the lives of immunocompromised people. Historically, knowledge has been drawn from studies on Saccharomyces cerevisiae to understand the pathogen, and many Candida albicans genes are named after their S. cerevisiae orthologs. Direct studies on the pathogen have, however, revealed differences in the roles of some orthologous proteins in the two yeasts. We show that the Put3 transcription factor allows the pathogen to completely degrade proline to usable nitrogen and carbon by evading regulatory restrictions imposed on its S. cerevisiae ortholog, which mandates conditional use of proline only as a nitrogen source in the baker’s yeast. The ability of Candida albicans to freely obtain nutrients from multiple sources may help it thrive as a commensal and opportunistic pathogen. The zinc cluster transcription factor Put3 was initially characterized in Saccharomyces cerevisiae as the transcriptional activator of PUT1 and PUT2, two genes acting early in the proline assimilation pathway. We have used phenotypic studies, transcription profiling, and chromatin immunoprecipitation with microarray technology (ChIP-chip) to establish that unlike S. cerevisiae, which only uses proline as a nitrogen source, Candida albicans can use proline as a nitrogen source, a carbon source, or a source of both nitrogen and carbon. However, a C. albicans put3 null mutant cannot grow on proline, suggesting that as in S. cerevisiae, C. albicans Put3 (CaPut3) is required for proline catabolism, and because the C. albicans put3 null mutant grew efficiently on glutamate as the sole carbon or nitrogen source, it appears that CaPut3 also regulates the early genes of the pathway. CaPut3 showed direct binding to the CaPUT1 promoter, and both PUT1 and PUT2 were upregulated in response to proline addition in a Put3-dependent manner, as well as in a C. albicans strain expressing a hyperactive Put3. CaPut3 directs proline degradation even in the presence of a good nitrogen source such as ammonia, which contrasts with S. cerevisiae Put3 (ScPut3)-regulated proline catabolism, which only occurs in the absence of a rich nitrogen source. Thus, while overall proline regulatory circuitry differs between S. cerevisiae and C. albicans, the specific role of Put3 appears fundamentally conserved. IMPORTANCE Candida albicans poses a significant threat to the lives of immunocompromised people. Historically, knowledge has been drawn from studies on Saccharomyces cerevisiae to understand the pathogen, and many Candida albicans genes are named after their S. cerevisiae orthologs. Direct studies on the pathogen have, however, revealed differences in the roles of some orthologous proteins in the two yeasts. We show that the Put3 transcription factor allows the pathogen to completely degrade proline to usable nitrogen and carbon by evading regulatory restrictions imposed on its S. cerevisiae ortholog, which mandates conditional use of proline only as a nitrogen source in the baker’s yeast. The ability of Candida albicans to freely obtain nutrients from multiple sources may help it thrive as a commensal and opportunistic pathogen.

Proteomic and functional analysis of proline dehydrogenase 1 link proline catabolism to mitochondrial electron transport in Arabidopsis thaliana

2016 ◽  
Vol 473 (17) ◽  
pp. 2623-2634 ◽  
Author(s):  
Cécile Cabassa-Hourton ◽  
Peter Schertl ◽  
Marianne Bordenave-Jacquemin ◽  
Kaouthar Saadallah ◽  
Anne Guivarc'h ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Arabidopsis Thaliana ◽  
Respiratory Chain ◽  
Chain Complex ◽  
Respiratory Chain Complex ◽  
Proline Dehydrogenase ◽  
Mitochondrial Membranes ◽  
Proline Catabolism ◽  
Biochemical Analyses

Molecular and biochemical analyses indicate a key role of proline dehydrogenase 1 in proline oxidation. ProDH1 binds to mitochondrial membranes, is part of small respiratory chain complex and delivers its electrons to the respiratory chain.

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