Proteomic and functional analysis of proline dehydrogenase 1 link proline catabolism to mitochondrial electron transport in Arabidopsis thaliana

2016 ◽  
Vol 473 (17) ◽  
pp. 2623-2634 ◽  
Author(s):  
Cécile Cabassa-Hourton ◽  
Peter Schertl ◽  
Marianne Bordenave-Jacquemin ◽  
Kaouthar Saadallah ◽  
Anne Guivarc'h ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Arabidopsis Thaliana ◽  
Respiratory Chain ◽  
Chain Complex ◽  
Respiratory Chain Complex ◽  
Proline Dehydrogenase ◽  
Mitochondrial Membranes ◽  
Proline Catabolism ◽  
Biochemical Analyses

Molecular and biochemical analyses indicate a key role of proline dehydrogenase 1 in proline oxidation. ProDH1 binds to mitochondrial membranes, is part of small respiratory chain complex and delivers its electrons to the respiratory chain.

scholarly journals Identification of novel genes involved in apoptosis of HIV-infected macrophages using unbiased genome-wide screening

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Simon X. M. Dong ◽  
Frederick S. Vizeacoumar ◽  
Kalpana K. Bhanumathy ◽  
Nezeka Alli ◽  
Cristina Gonzalez-Lopez ◽  
...  
Keyword(s):  
T Cells ◽  
Hiv Infection ◽  
Respiratory Chain ◽  
Cd4 T Cells ◽  
Chain Complex ◽  
Respiratory Chain Complex ◽  
Complex Ii ◽  
Genome Wide ◽  
Latently Infected ◽  
Induced Apoptosis

Abstract Background Macrophages, besides resting latently infected CD4+ T cells, constitute the predominant stable, major non-T cell HIV reservoirs. Therefore, it is essential to eliminate both latently infected CD4+ T cells and tissue macrophages to completely eradicate HIV in patients. Until now, most of the research focus is directed towards eliminating latently infected CD4+ T cells. However, few approaches have been directed at killing of HIV-infected macrophages either in vitro or in vivo. HIV infection dysregulates the expression of many host genes essential for the survival of infected cells. We postulated that exploiting this alteration may yield novel targets for the selective killing of infected macrophages. Methods We applied a pooled shRNA-based genome-wide approach by employing a lentivirus-based library of shRNAs to screen novel gene targets whose inhibition should selectively induce apoptosis in HIV-infected macrophages. Primary human MDMs were infected with HIV-eGFP and HIV-HSA viruses. Infected MDMs were transfected with siRNAs specific for the promising genes followed by analysis of apoptosis by flow cytometry using labelled Annexin-V in HIV-infected, HIV-exposed but uninfected bystander MDMs and uninfected MDMs. The results were analyzed using student’s t-test from at least four independent experiments. Results We validated 28 top hits in two independent HIV infection models. This culminated in the identification of four target genes, Cox7a2, Znf484, Cstf2t, and Cdk2, whose loss-of-function induced apoptosis preferentially in HIV-infected macrophages. Silencing these single genes killed significantly higher number of HIV-HSA-infected MDMs compared to the HIV-HSA-exposed, uninfected bystander macrophages, indicating the specificity in the killing of HIV-infected macrophages. The mechanism governing Cox7a2-mediated apoptosis of HIV-infected macrophages revealed that targeting respiratory chain complex II and IV genes also selectively induced apoptosis of HIV-infected macrophages possibly through enhanced ROS production. Conclusions We have identified above-mentioned novel genes and specifically the respiratory chain complex II and IV genes whose silencing may cause selective elimination of HIV-infected macrophages and eventually the HIV-macrophage reservoirs. The results highlight the potential of the identified genes as targets for eliminating HIV-infected macrophages in physiological environment as part of an HIV cure strategy.

scholarly journals NAD + repletion produces no therapeutic effect in mice with respiratory chain complex III deficiency and chronic energy deprivation

2018 ◽  
Vol 32 (11) ◽  
pp. 5913-5926 ◽  
Author(s):  
Janne Purhonen ◽  
Jayasimman Rajendran ◽  
Saara Tegelberg ◽  
Olli-Pekka Smolander ◽  
Eija Pirinen ◽  
...  
Keyword(s):  
Therapeutic Effect ◽  
Respiratory Chain ◽  
Chain Complex ◽  
Complex Iii ◽  
Respiratory Chain Complex ◽  
Energy Deprivation

scholarly journals The NADH-binding subunit of respiratory chain complex I is nuclear-encoded in plants and identified only in mitochondria

1996 ◽  
Vol 10 (5) ◽  
pp. 793-803 ◽  
Author(s):  
Lutz Grohmann ◽  
Allan G. Rasmusson ◽  
Volker Heiser ◽  
Oliver Thieck ◽  
Axel Brennicke
Keyword(s):  
Respiratory Chain ◽  
Complex I ◽  
Chain Complex ◽  
Respiratory Chain Complex ◽  
Binding Subunit
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