scholarly journals Defective early innate immune response to ectromelia virus in the draining lymph nodes of aged mice due to impaired dendritic cell accumulation

Aging Cell ◽  
2020 ◽  
Vol 19 (7) ◽  
Author(s):  
Colby Stotesbury ◽  
Eric B. Wong ◽  
Lingjuan Tang ◽  
Brian Montoya ◽  
Cory J. Knudson ◽  
...  
Keyword(s):  
Immune Response ◽  
Dendritic Cell ◽  
Lymph Nodes ◽  
Innate Immune Response ◽  
Innate Immune ◽  
Ectromelia Virus ◽  
Aged Mice ◽  
Cell Accumulation ◽  
Draining Lymph Nodes

scholarly journals Expression kinetics and innate immune response after electroporation and lipid nanoparticle mediated delivery of a self-amplifying mRNA in the skin of mice

2019 ◽  
Author(s):  
Hanne Huysmans ◽  
Zifu Zhong ◽  
Joyca De Temmerman ◽  
Barbara L. Mui ◽  
Ying K. Tam ◽  
...  
Keyword(s):  
Immune Response ◽  
Protein Expression ◽  
Lymph Nodes ◽  
Innate Immune Response ◽  
Intradermal Injection ◽  
Innate Immune ◽  
Sharp Drop ◽  
Lipid Nanoparticle ◽  
Draining Lymph Nodes ◽  
Expression Kinetics

AbstractIn this work we studied the expression kinetics and innate immune response of a self-amplifying mRNA (sa-RNA) after electroporation and lipid nanoparticle (LNP) mediated delivery in the skin of mice. Intradermal electroporation of the sa-RNA resulted in a plateau-shaped expression with the plateau between day 3 and 10. The overall protein expression of sa-RNA was significant higher than that obtained after electroporation of pDNA or non-replication mRNAs. Moreover, intradermal electroporation of sa-RNA induced a short-lived innate immune response that did not affect the expression of the sa-RNA. A complete different expression profile and innate immune response was observed when LNPs were used. The expression peaked 24h after intradermal injection of sa-RNA-LNPs and subsequently showed a sharp drop. This drop can be explained by the strong innate immune response elicited by the sa-RNA-LNPs 4h after injection. Interestingly, sa-RNA-LNPs were able to transfection the draining lymph nodes after intradermal injection.

scholarly journals Effects of Regulatory T Cell Depletion on NK Cell Responses against Listeria monocytogenes in Feline Immunodeficiency Virus Infected Cats

Viruses ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 984
Author(s):  
Simões ◽  
LaVoy ◽  
Dean
Keyword(s):  
Immune Response ◽  
Listeria Monocytogenes ◽  
Dendritic Cell ◽  
Innate Immune Response ◽  
Feline Immunodeficiency Virus ◽  
Innate Immune ◽  
Treg Depletion ◽  
Cell Responses ◽  
Immunodeficiency Virus

Regulatory T cells (Treg) are key players in the maintenance of peripheral tolerance, preventing autoimmune diseases and restraining chronic inflammatory diseases. Evidence suggests Treg cells and NK cells have important roles in feline immunodeficiency virus (FIV) pathogenesis; however, in vivo studies investigating the interplay between these two cell populations are lacking. We previously described innate immune defects in FIV-infected cats characterized by cytokine deficits and impaired natural killer cell (NK) and NK T cell (NKT) functions. In this study, we investigated whether in vivo Treg depletion by treatment with an anti-feline CD25 monoclonal antibody would improve the innate immune response against subcutaneous challenge with Listeria monocytogenes (Lm). Treg depletion resulted in an increased overall number of cells in Lm-draining lymph nodes and increased proliferation of NK and NKT cells in FIV-infected cats. Treg depletion did not normalize expression of perforin or granzyme A by NK and NKT cells, nor did Treg depletion result in improved clearance of Lm. Thus, despite the quantitative improvements in the NK and NKT cell responses to Lm, there was no functional improvement in the early control of Lm. CD1a+ dendritic cell percentages in the lymph nodes of FIV-infected cats were lower than in specific-pathogen-free control cats and failed to upregulate CD80 even when Treg were depleted. Taken together, Treg depletion failed to improve the innate immune response of FIV-infected cats against Lm and this may be due to dendritic cell dysfunction.

scholarly journals A Spatially-Organized Multicellular Innate Immune Response in Lymph Nodes Limits Systemic Pathogen Spread

Cell ◽  
2012 ◽  
Vol 150 (6) ◽  
pp. 1235-1248 ◽  
Author(s):  
Wolfgang Kastenmüller ◽  
Parizad Torabi-Parizi ◽  
Naeha Subramanian ◽  
Tim Lämmermann ◽  
Ronald N. Germain
Keyword(s):  
Immune Response ◽  
Lymph Nodes ◽  
Innate Immune Response ◽  
Innate Immune

scholarly journals The Early Activation ofCD8+T Cells Is Dependent on Type I IFN Signaling following Intramuscular Vaccination of Adenovirus Vector

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Masahisa Hemmi ◽  
Masashi Tachibana ◽  
Sayaka Tsuzuki ◽  
Masaki Shoji ◽  
Fuminori Sakurai ◽  
...  
Keyword(s):  
Immune Response ◽  
Lymph Nodes ◽  
Cytotoxic T Lymphocytes ◽  
Adenovirus Vector ◽  
Innate Immune ◽  
Type I ◽  
Type I Ifn ◽  
Early Activation ◽  
Innate Immune Signaling ◽  
Draining Lymph Nodes

Few of the vaccines in current use can induce antigen- (Ag-) specific immunity in both mucosal and systemic compartments. Hence, the development of vaccines that realize both mucosal and systemic protection against various pathogens is a high priority in global health. Recently, it has been reported that intramuscular (i.m.) vaccination of an adenovirus vector (Adv) can induce Ag-specific cytotoxic T lymphocytes (CTLs) in both systemic and gut mucosal compartments. We previously revealed that type I IFN signaling is required for the induction of gut mucosal CTLs, not systemic CTLs. However, the molecular mechanism via type I IFN signaling is largely unknown. Here, we report that type I IFN signaling following i.m. Adv vaccination is required for the expression of type I IFN in the inguinal lymph nodes (iLNs), which are the draining lymph nodes of the administration site. We also showed that the type I IFN signaling is indispensable for the early activation of CTLs in iLNs. These data suggested that type I IFN signaling has an important role in the translation of systemic innate immune response into mucosal adaptive immunity by amplifying the innate immune signaling and activating CTLs in the iLN.

scholarly journals Aminated nanomicelles as a designer vaccine adjuvant to trigger inflammasomes and multiple arms of the innate immune response in lymph nodes

2017 ◽  
Vol Volume 12 ◽  
pp. 7501-7517 ◽  
Author(s):  
Chanyoung Song ◽  
Hathaichanok Phuengkham ◽  
Sun-Young Kim ◽  
Min Sang Lee ◽  
Ji Hoon Jeong ◽  
...  
Keyword(s):  
Immune Response ◽  
Lymph Nodes ◽  
Innate Immune Response ◽  
Innate Immune ◽  
Vaccine Adjuvant

scholarly journals The Innate Immune Response to Infection Induces Erythropoietin-Dependent Replenishment of the Dendritic Cell Compartment

2020 ◽  
Vol 11 ◽  
Author(s):  
Henrik Einwächter ◽  
Alexander Heiseke ◽  
Andreas Schlitzer ◽  
Georg Gasteiger ◽  
Heiko Adler ◽  
...  
Keyword(s):  
Immune Response ◽  
Dendritic Cell ◽  
Innate Immune Response ◽  
Innate Immune ◽  
Cell Compartment

scholarly journals The cGas–Sting Signaling Pathway Is Required for the Innate Immune Response Against Ectromelia Virus

2018 ◽  
Vol 9 ◽  
Author(s):  
Wen-Yu Cheng ◽  
Xiao-Bing He ◽  
Huai-Jie Jia ◽  
Guo-Hua Chen ◽  
Qi-Wang Jin ◽  
...  
Keyword(s):  
Immune Response ◽  
Signaling Pathway ◽  
Innate Immune Response ◽  
Innate Immune ◽  
Ectromelia Virus
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