scholarly journals Geriatric fragility fractures are associated with a human skeletal stem cell defect

Aging Cell ◽  
2020 ◽  
Vol 19 (7) ◽  
Author(s):  
Thomas H. Ambrosi ◽  
L. Henry Goodnough ◽  
Holly M. Steininger ◽  
Malachia Y. Hoover ◽  
Emiley Kim ◽  
...  
Keyword(s):  
Stem Cell ◽  
Fragility Fractures ◽  
Skeletal Stem Cell ◽  
Cell Defect

Close to the bone — in search of the skeletal stem cell

2018 ◽  
Vol 14 (12) ◽  
pp. 687-688 ◽  
Author(s):  
Frank P. Luyten ◽  
Scott J. Roberts
Keyword(s):  
Stem Cell ◽  
Skeletal Stem Cell

Stem Cell Defect in Ubiquitin-Green Fluorescent Protein Mice Facilitates Engraftment of Lymphoid-Primed Hematopoietic Stem Cells

Stem Cells ◽  
2018 ◽  
Vol 36 (8) ◽  
pp. 1237-1248
Author(s):  
Kateřina Faltusová ◽  
Katarína Szikszai ◽  
Martin Molík ◽  
Jana Linhartová ◽  
Petr Páral ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Green Fluorescent Protein ◽  
Hematopoietic Stem Cells ◽  
Fluorescent Protein ◽  
Hematopoietic Stem ◽  
Green Fluorescent ◽  
Cell Defect

In search of the skeletal stem cell: isolation and separation strategies at the macro/micro scale for skeletal regeneration

Lab on a Chip ◽  
2011 ◽  
Vol 11 (7) ◽  
pp. 1206 ◽  
Author(s):  
David Gothard ◽  
Rahul S. Tare ◽  
Peter D. Mitchell ◽  
Jonathan I. Dawson ◽  
Richard O. C. Oreffo
Keyword(s):  
Stem Cell ◽  
Cell Isolation ◽  
Micro Scale ◽  
Stem Cell Isolation ◽  
Skeletal Regeneration ◽  
Skeletal Stem Cell

Graft vascularization is a critical rate-limiting step in skeletal stem cell-mediated posterolateral spinal fusion

2009 ◽  
Vol 4 (4) ◽  
pp. 273-283 ◽  
Author(s):  
Giuseppe Giannicola ◽  
Emiliano Ferrari ◽  
Gennaro Citro ◽  
Benedetto Sacchetti ◽  
Alessandro Corsi ◽  
...  
Keyword(s):  
Stem Cell ◽  
Spinal Fusion ◽  
Critical Rate ◽  
Rate Limiting Step ◽  
Limiting Step ◽  
Posterolateral Spinal Fusion ◽  
Rate Limiting ◽  
Skeletal Stem Cell

Cell Intrinsic versus Extrinsic Factors and their Role in Skeletal Stem Cell Function with Aging

2014 ◽  
Vol 186 (2) ◽  
pp. 689
Author(s):  
A. McArdle ◽  
C.K. Chan ◽  
D.D. Lo ◽  
M.T. Chung ◽  
K. Senarath-Yapa ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Function ◽  
Extrinsic Factors ◽  
Skeletal Stem Cell

scholarly journals Incomplete restoration of Mpl expression in the mpl−/− mouse produces partial correction of the stem cell–repopulating defect and paradoxical thrombocytosis

Blood ◽  
2009 ◽  
Vol 113 (8) ◽  
pp. 1778-1785 ◽  
Author(s):  
Brian J. Lannutti ◽  
Angela Epp ◽  
Jacqueline Roy ◽  
Junmei Chen ◽  
Neil C. Josephson
Keyword(s):  
Stem Cell ◽  
Elevated Plasma ◽  
Platelet Production ◽  
Ectopic Gene Expression ◽  
Unexpected Consequence ◽  
Kinase Phosphorylation ◽  
Bone Marrow Chimeras ◽  
Partial Correction ◽  
Cell Defect ◽  
Expression And Activity

Abstract Expression of Mpl is restricted to hematopoietic cells in the megakaryocyte lineage and to undifferentiated progenitors, where it initiates critical cell survival and proliferation signals after stimulation by its ligand, thrombopoietin (TPO). As a result, a deficiency in Mpl function in patients with congenital amegakaryocytic thrombocytopenia (CAMT) and in mpl−/− mice produces profound thrombocytopenia and a severe stem cell–repopulating defect. Gene therapy has the potential to correct the hematopoietic defects of CAMT by ectopic gene expression that restores normal Mpl receptor activity. We rescued the mpl−/− mouse with a transgenic vector expressing mpl from the promoter elements of the 2-kb region of DNA just proximal to the natural gene start site. Transgene rescued mice exhibit thrombocytosis but only partial correction of the stem cell defect. Furthermore, they show very low-level expression of Mpl on platelets and megakaryocytes, and the transgene-rescued megakaryocytes exhibit diminished TPO-dependent kinase phosphorylation and reduced platelet production in bone marrow chimeras. Thrombocytosis is an unexpected consequence of reduced Mpl expression and activity. However, impaired TPO homeostasis in the transgene-rescued mice produces elevated plasma TPO levels, which serves as an unchecked stimulus to drive the observed excessive megakaryocytopoiesis.

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