scholarly journals Role of Phe283 in enzymatic reaction of cyclodextrin glycosyltransferase from alkalophilic Bacillus sp.1011: Substrate binding and arrangement of the catalytic site

2004 ◽  
Vol 13 (2) ◽  
pp. 457-465 ◽  
Author(s):  
R. Kanai
Keyword(s):  
Substrate Binding ◽  
Enzymatic Reaction ◽  
Catalytic Site ◽  
Bacillus Sp ◽  
Cyclodextrin Glycosyltransferase ◽  
Alkalophilic Bacillus

scholarly journals Byproducts from cassava industry: alternative substrates for cyclodextrin glycosyltransferase production by alkalophilic Bacillus trypoxylicola SM–02

2020 ◽  
pp. 37-46
Author(s):  
Carine Mascena Peixoto ◽  
Sheila Lorena de Araújo Coelho ◽  
Marcia Luciana Cazetta
Keyword(s):  
Corn Steep Liquor ◽  
Relative Activity ◽  
Optimum Temperature ◽  
Cyclodextrin Glycosyltransferase ◽  
Cassava Wastewater ◽  
Steep Liquor ◽  
Alkalophilic Bacillus ◽  
Central Composite ◽  
Cassava Peel

Bioproductos de la industria de la yuca: sustratos alternativos para la producción de ciclodextrina glucosiltransferasa por alcalófilo Bacillus trypoxylicola SM-02 En el presente trabajo estudiamos el uso licor de maíz fermentado (LMF), harina de cáscara de yuca (HCY) y aguas residuales de yuca para la producción de ciclodextina glicosiltransferasa (CGTase) por un nuevo aislado alcalófilo de Bacillus trypoxylicola SM-02 en fermentación sumergida. Los experimentos se realizaron por Diseño Central Compuesto Rotativo 22 totalizando 11 ensayos. La mayor actividad enzimática de 352.53 U/mL se obtuvo con 1.5 g de HCY y 0.6 g de LMF. La temperatura y el pH óptimos fueron 55 ºC y pH 8.0, respectivamente. CGTase mostró una actividad relativa superior al 50% durante 120 min. a la temperatura óptima. Solo el CaCl2 mostró actividad positiva para CGTasa. Los resultados apuntaron a un buen potencial de B. trypoxylicola SM-02 para la producción de CGTasa usando substratos residuales. In the present work was studied the use of cassava peel flour (CPF), corn steep liquor (CSL), and cassava wastewater as substrates to produce cyclodextrin glycosyltransferase (CGTase) from a new alkalophilic isolate of Bacillus trypoxylicola SM-02 by submerged fermentation. The experiments were performed as a Central Composite Design 22, totalizing 11 assays. An enzymatic activity of 352.53 U/mL was obtained using 1.5 g of CPF and 0.6 g of CSL. The optimum temperature and pH of CGTase was 55 °C and 8.0, respectively. The CGTase depicted a relative activity of more than 50% for 120 min at the optimum temperature. The only salt that positively influenced the CGTase activity was CaCl2. The results are indicative of a potential role of B. trypoxylicola SM-02 in the production of CGTase using residual substrates.

Sign in / Sign up

Export Citation Format

Share Document