An ultrasound imaging method for in vivo measurement of tracheal elasticity

2008 ◽  
Author(s):  
Chih-Yen Chen ◽  
Chao-Ling Wu ◽  
Shou Chia Chu ◽  
Huihua Kenny Chiang
Keyword(s):  
Ultrasound Imaging ◽  
Imaging Method ◽  
In Vivo Measurement

Ultrasound Biomicroscopy of Cancer Therapy Effects: Correlation between Light and Electron Microscopy, and a New Non-Invasive Ultrasound Imaging Method for Detecting Apoptosis

2000 ◽  
Vol 6 (S2) ◽  
pp. 1014-1015
Author(s):  
G.J. Czarnota ◽  
M.C. Kolios ◽  
Y.M. Heng ◽  
K. Devaraj ◽  
C. Tam ◽  
...  
Keyword(s):  
Cell Death ◽  
Photodynamic Therapy ◽  
Ultrasound Imaging ◽  
Light And Electron Microscopy ◽  
Ultrasound Biomicroscopy ◽  
Response To Treatment ◽  
Imaging Method ◽  
High Frequency Ultrasound

We have discovered that high-frequency ultrasound imaging, or ultrasound biomicroscopy, can be used to detect apoptosis in a number of experimental systems. We have shown that such detection with 30-40 MHz ultrasound is possible using cells in an in vitro system (AML-3 leukemia cells) made to undergo apoptosis in response to treatment with a variety of cancer killing chemotherapeutic drugs. We have shown that ultrasound biomicroscopy can also detect programmed cell death in tissues made to undergo apoptosis in response to photodynamic therapy, currently an experimental cancer treating regimen. Lastly, we have shown that this ultrasound imaging approach works in vivo, using living animals where apoptosis has been induced similarly using photodynamic therapy. Specifically, apoptotic cells and regions of apoptosis in tissues exhibit up to a 36-fold increase in ultrasound backscatter intensity permitting this type of cell death to be readily discriminated from surrounding viable tissue.

An ultrasound imaging method for in vivo tracheal bulk and Young's moduli of elasticity

2007 ◽  
Vol 40 (7) ◽  
pp. 1615-1621 ◽  
Author(s):  
Thomas L. Miller ◽  
Allison R. Altman ◽  
Takeshi Tsuda ◽  
Thomas H. Shaffer
Keyword(s):  
Ultrasound Imaging ◽  
Imaging Method ◽  
Young’S Moduli ◽  
Moduli Of Elasticity

In-Vivo Measurement of Vitreous Motion Using an Ultrasound Imaging Velocimetry Technique

2012 ◽  
Author(s):  
Tommaso Rossi ◽  
Giorgio Querzoli ◽  
Rodolfo Repetto ◽  
Alessandro Stocchino ◽  
Giacomo Pasqualitto ◽  
...  
Keyword(s):  
Retinal Detachment ◽  
Retinal Degeneration ◽  
Ultrasound Imaging ◽  
Quantitative Measurement ◽  
Vitreous Haemorrhage ◽  
Retinal Diseases ◽  
In Vivo Measurement ◽  
The Past ◽  
Vitreous Dynamics

Vitreous dynamics plays a fundamental role in the pathogenesis of various vitreo-retinal diseases, such as vitreous haemorrhage, peripheral retinal degeneration and tears, retinal detachment,… Quantitative measurement of vitreous motion has been attempted in the past using ultrasound imaging [1] and MRI [2]. However, information about vitreous motion secondary to eye rotations as well as knowledge of vitreous rheology is still limited.

scholarly journals In Vivo Venous Assessment of Red Blood Cell Aggregate Sizes in Diabetic Patients with a Quantitative Cellular Ultrasound Imaging Method: Proof of Concept

PLoS ONE ◽  
2015 ◽  
Vol 10 (4) ◽  
pp. e0124712 ◽  
Author(s):  
Julien Tripette ◽  
Linh-Chi Nguyen ◽  
Louise Allard ◽  
Pierre Robillard ◽  
Gilles Soulez ◽  
...  
Keyword(s):  
Blood Cell ◽  
Ultrasound Imaging ◽  
Red Blood Cell ◽  
Diabetic Patients ◽  
Imaging Method ◽  
Cell Aggregate ◽  
Proof Of Concept

scholarly journals High-speed volumetric two-photon fluorescence imaging of neurovascular dynamics

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Jiang Lan Fan ◽  
Jose A. Rivera ◽  
Wei Sun ◽  
John Peterson ◽  
Henry Haeberle ◽  
...  
Keyword(s):  
Blood Flow ◽  
High Speed ◽  
Laser Scanning ◽  
Three Dimensional ◽  
Laser Scanning Microscopy ◽  
Fluorescence Signal ◽  
Imaging Method ◽  
Spatiotemporal Resolution ◽  
Two Photon

AbstractUnderstanding the structure and function of vasculature in the brain requires us to monitor distributed hemodynamics at high spatial and temporal resolution in three-dimensional (3D) volumes in vivo. Currently, a volumetric vasculature imaging method with sub-capillary spatial resolution and blood flow-resolving speed is lacking. Here, using two-photon laser scanning microscopy (TPLSM) with an axially extended Bessel focus, we capture volumetric hemodynamics in the awake mouse brain at a spatiotemporal resolution sufficient for measuring capillary size and blood flow. With Bessel TPLSM, the fluorescence signal of a vessel becomes proportional to its size, which enables convenient intensity-based analysis of vessel dilation and constriction dynamics in large volumes. We observe entrainment of vasodilation and vasoconstriction with pupil diameter and measure 3D blood flow at 99 volumes/second. Demonstrating high-throughput monitoring of hemodynamics in the awake brain, we expect Bessel TPLSM to make broad impacts on neurovasculature research.

scholarly journals In Vivo Measurement of Neurochemical Abnormalities in the Hippocampus in a Rat Model of Cuprizone-Induced Demyelination

Diagnostics ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 45
Author(s):  
Do-Wan Lee ◽  
Jae-Im Kwon ◽  
Chul-Woong Woo ◽  
Hwon Heo ◽  
Kyung Won Kim ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Rat Model ◽  
Resonance Spectroscopy ◽  
Chow Diet ◽  
Gamma Aminobutyric Acid ◽  
Sprague Dawley ◽  
In Vivo Measurement ◽  
Hippocampal Lesions ◽  
Total Creatine

This study quantitatively measured the changes in metabolites in the hippocampal lesions of a rat model of cuprizone-induced demyelination as detected using in vivo 7 T proton magnetic resonance spectroscopy. Nineteen Sprague Dawley rats were randomly divided into two groups and fed a normal chow diet or cuprizone (0.2%, w/w) for 7 weeks. Demyelinated hippocampal lesions were quantitatively measured using a 7 T magnetic resonance imaging scanner. All proton spectra were quantified for metabolite concentrations and relative ratios. Compared to those in the controls, the cuprizone-induced rats had significantly higher concentrations of glutamate (p = 0.001), gamma-aminobutyric acid (p = 0.019), and glutamate + glutamine (p = 0.001); however, creatine + phosphocreatine (p = 0.006) and myo-inositol (p = 0.001) concentrations were lower. In addition, we found that the glutamine and glutamate complex/total creatine (p < 0.001), glutamate/total creatine (p < 0.001), and GABA/total creatine (p = 0.002) ratios were significantly higher in cuprizone-treated rats than in control rats. Our results showed that cuprizone-induced neuronal demyelination may influence the severe abnormal metabolism in hippocampal lesions, and these responses could be caused by microglial activation, mitochondrial dysfunction, and astrocytic necrosis.

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