A fully integrated, high-throughput, multi-parametric flow cytometry chip using “microfluidic drifting” based three-dimensional (3D) hydrodynamic focusing

2011 ◽  
Author(s):  
Xiaole Mao ◽  
Ahmad Ahsan Nawaz ◽  
Tony Jun Huang
Keyword(s):  
Flow Cytometry ◽  
High Throughput ◽  
Three Dimensional ◽  
Hydrodynamic Focusing ◽  
Fully Integrated

scholarly journals Cameraless high-throughput three-dimensional imaging flow cytometry

Optica ◽  
2019 ◽  
Vol 6 (10) ◽  
pp. 1297 ◽  
Author(s):  
Yuanyuan Han ◽  
Rui Tang ◽  
Yi Gu ◽  
Alex Ce Zhang ◽  
Wei Cai ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
High Throughput ◽  
Three Dimensional ◽  
Three Dimensional Imaging ◽  
Imaging Flow Cytometry

scholarly journals An inexpensive microfluidic device for three-dimensional hydrodynamic focusing in imaging flow cytometry

2020 ◽  
Vol 14 (6) ◽  
pp. 064110
Author(s):  
Yogesh M. Patel ◽  
Sanidhya Jain ◽  
Abhishek Kumar Singh ◽  
Kedar Khare ◽  
Sarita Ahlawat ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Microfluidic Device ◽  
Three Dimensional ◽  
Hydrodynamic Focusing ◽  
Imaging Flow Cytometry

scholarly journals An integrated, multiparametric flow cytometry chip using “microfluidic drifting” based three-dimensional hydrodynamic focusing

2012 ◽  
Vol 6 (2) ◽  
pp. 024113 ◽  
Author(s):  
Xiaole Mao ◽  
Ahmad Ahsan Nawaz ◽  
Sz-Chin Steven Lin ◽  
Michael Ian Lapsley ◽  
Yanhui Zhao ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Three Dimensional ◽  
Hydrodynamic Focusing ◽  
Multiparametric Flow Cytometry

scholarly journals High-Throughput Flow Cytometry Drug Combination Discovery with Novel Synergy Analysis Software, SynScreen

2018 ◽  
Vol 23 (7) ◽  
pp. 751-760
Author(s):  
Dominique R. Perez ◽  
Bruce S. Edwards ◽  
Larry A. Sklar ◽  
Alexandre Chigaev
Keyword(s):  
Flow Cytometry ◽  
High Throughput ◽  
Drug Combination ◽  
Three Dimensional ◽  
Drug Combinations ◽  
Data Sets ◽  
Software Application ◽  
Dose Responses ◽  
Analysis Models

Classical therapeutic regimens are subject to toxicity, low efficacy, and/or the development of drug resistance. Thus, the discovery of synergistic drug combinations would permit treatment with lower, tolerable dosages of each agent and restored sensitivity. We describe the development and use of the SynScreen software application, which allows for visual and mathematical determinations of compound concentrations that produce super-additive effects. This software uses nonlinear regression fits of dose responses to determine synergism by the Bliss independence and Loewe additivity analysis models. We demonstrate the utility of SynScreen with data analysis from in vitro high-throughput flow cytometry (HTFC) combination screens with repurposed drugs and multiplexed synergy analysis of multiple biologic parameters in parallel. The applicability of SynScreen was confirmed by testing open-source data sets used in published drug combination literature. A key benefit of SynScreen for high-throughput drug combination screening is that observed measurements are graphically depicted in comparison with a three-dimensional surface that represents the theoretical responses at which Bliss additivity would occur. These images and summary tables for the calculated drug interactions are automatically exported. This allows for substantial data sets to be visually assessed, expediting the quick identification of efficacious drug combinations and thereby facilitating the design of confirmatory studies and clinical trials.

A deep learning-based concept for high throughput image flow cytometry

2021 ◽  
Vol 118 (12) ◽  
pp. 123701
Author(s):  
Julie Martin-Wortham ◽  
Steffen M. Recktenwald ◽  
Marcelle G. M. Lopes ◽  
Lars Kaestner ◽  
Christian Wagner ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Deep Learning ◽  
High Throughput ◽  
Image Flow

scholarly journals A 3D hydrodynamic flow-focusing device for cell sorting

2021 ◽  
Vol 25 (3) ◽  
Author(s):  
Xiaofei Yuan ◽  
Andrew Glidle ◽  
Hitoshi Furusho ◽  
Huabing Yin
Keyword(s):  
Cell Sorting ◽  
Control Strategies ◽  
Three Dimensional ◽  
Hydrodynamic Flow ◽  
Proof Of Concept ◽  
Flow Focusing ◽  
Hydrodynamic Focusing ◽  
Cell Handling ◽  
Fluid Flow Control ◽  
High Degree

AbstractOptical-based microfluidic cell sorting has become increasingly attractive for applications in life and environmental sciences due to its ability of sophisticated cell handling in flow. The majority of these microfluidic cell sorting devices employ two-dimensional fluid flow control strategies, which lack the ability to manipulate the position of cells arbitrarily for precise optical detection, therefore resulting in reduced sorting accuracy and purity. Although three-dimensional (3D) hydrodynamic devices have better flow-focusing characteristics, most lack the flexibility to arbitrarily position the sample flow in each direction. Thus, there have been very few studies using 3D hydrodynamic flow focusing for sorting. Herein, we designed a 3D hydrodynamic focusing sorting platform based on independent sheath flow-focusing and pressure-actuated switching. This design offers many advantages in terms of reliable acquisition of weak Raman signals due to the ability to precisely control the speed and position of samples in 3D. With a proof-of-concept demonstration, we show this 3D hydrodynamic focusing-based sorting device has the potential to reach a high degree of accuracy for Raman activated sorting.

scholarly journals High-throughput multiparametric imaging flow cytometry: toward diffraction-limited sub-cellular detection and monitoring of sub-cellular processes

Cell Reports ◽  
2021 ◽  
Vol 34 (10) ◽  
pp. 108824
Author(s):  
Gregor Holzner ◽  
Bogdan Mateescu ◽  
Daniel van Leeuwen ◽  
Gea Cereghetti ◽  
Reinhard Dechant ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
High Throughput ◽  
Multiparametric Imaging ◽  
Cellular Processes ◽  
Imaging Flow Cytometry

scholarly journals A “No‐Touch” Antibody‐Staining Method of Adherent Cells for High‐Throughput Flow Cytometry in 384‐Well Microplate Format for Cell‐Based Drug Library Screening

2019 ◽  
Vol 97 (8) ◽  
pp. 845-851 ◽  
Author(s):  
Annelisa M. Cornel ◽  
Celina L. Szanto ◽  
Niek P. Til ◽  
Jeroen F. Velzen ◽  
Jaap J. Boelens ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
High Throughput ◽  
Staining Method ◽  
Adherent Cells ◽  
Library Screening ◽  
Antibody Staining
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