Label-free real time imaging of neural communication using acetylcholine image sensor

2011 ◽  
Author(s):  
Shoko Takenaga ◽  
Yui Tamai ◽  
Kengo Hirai ◽  
Kazuhiro Takahashi ◽  
Takashi Sakurai ◽  
...  
Keyword(s):  
Real Time ◽  
Image Sensor ◽  
Label Free ◽  
Real Time Imaging ◽  
Neural Communication

scholarly journals Label-free real-time imaging of myelination in theXenopus laevistadpole byin vivostimulated Raman scattering microscopy

2014 ◽  
Vol 19 (8) ◽  
pp. 086005 ◽  
Author(s):  
Chun-Rui Hu ◽  
Delong Zhang ◽  
Mikhail N. Slipchenko ◽  
Ji-Xin Cheng ◽  
Bing Hu
Keyword(s):  
Raman Scattering ◽  
Real Time ◽  
Label Free ◽  
Real Time Imaging

scholarly journals Advanced Biosensing towards Real-Time Imaging of Protein Secretion from Single Cells

2020 ◽  
Author(s):  
Lang Zhou ◽  
Pengyu Chen ◽  
Aleksandr Simonian
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Protein Secretion ◽  
Intercellular Communication ◽  
Single Cells ◽  
Bone Marrow Failure ◽  
Cellular Protein ◽  
Label Free ◽  
Immune Effector ◽  
Real Time Imaging

Protein secretion of cells plays a vital role in intercellular communication. The abnormality and dysfunction of cellular protein secretion are associated with various physiological disorders, such as malignant proliferation of cells, aberrant immune function, and bone marrow failure. The heterogeneity of protein secretion exists not only between varying populations of cells, but also in the same phenotype of cells. Therefore, characterization of protein secretion from single cell contributes not only to the understanding of intercellular communication in immune effector, carcinogenesis and metastasis, but also to the development and improvement of diagnosis and therapy of relative diseases. In spite of abundant highly sensitive methods that have been developed for the detection of secreted proteins, majority of them fall short in providing sufficient spatial and temporal resolution for comprehensive profiling of protein secretion from single cells. The real-time imaging techniques allow rapid acquisition and manipulation of analyte information on a 2D plane, providing high spatiotemporal resolution. Here, we summarize recent advances in real-time imaging of secretory proteins from single cell, including label-free and labelling techniques, shedding light on the development of simple yet powerful methodology for real-time imaging of single-cell protein secretion.

Label free bio image sensor for real time monitoring of potassium ion released from hippocampal slices

2014 ◽  
Vol 201 ◽  
pp. 439-443 ◽  
Author(s):  
Akiteru Kono ◽  
Takashi Sakurai ◽  
Toshiaki Hattori ◽  
Koichi Okumura ◽  
Makoto Ishida ◽  
...  
Keyword(s):  
Real Time ◽  
Hippocampal Slices ◽  
Image Sensor ◽  
Potassium Ion ◽  
Label Free ◽  
Real Time Monitoring

Label-free real-time imaging in microchip free-flow electrophoresis applying high speed deep UV fluorescence scanning

Lab on a Chip ◽  
2012 ◽  
Vol 12 (3) ◽  
pp. 458-463 ◽  
Author(s):  
Stefan Köhler ◽  
Stefan Nagl ◽  
Stefanie Fritzsche ◽  
Detlev Belder
Keyword(s):  
Real Time ◽  
High Speed ◽  
Free Flow ◽  
Label Free ◽  
Real Time Imaging ◽  
Uv Fluorescence ◽  
Deep Uv
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