scholarly journals Label-free and real-time imaging of dehydration-induced DNA conformational changes in cellular nucleus using second harmonic microscopy

2014 ◽  
Vol 4 (1) ◽  
Author(s):  
Shuangmu Zhuo ◽  
Ming Ni
Keyword(s):  
Real Time ◽  
Conformational Changes ◽  
Second Harmonic ◽  
Label Free ◽  
Real Time Imaging ◽  
Dna Conformational Changes

scholarly journals Label-free real-time imaging of myelination in theXenopus laevistadpole byin vivostimulated Raman scattering microscopy

2014 ◽  
Vol 19 (8) ◽  
pp. 086005 ◽  
Author(s):  
Chun-Rui Hu ◽  
Delong Zhang ◽  
Mikhail N. Slipchenko ◽  
Ji-Xin Cheng ◽  
Bing Hu
Keyword(s):  
Raman Scattering ◽  
Real Time ◽  
Label Free ◽  
Real Time Imaging

scholarly journals Mapping of real-time morphological changes in the neuronal cytoskeleton with label-free wide-field second-harmonic imaging: a case study of nocodazole

2019 ◽  
Vol 6 (04) ◽  
pp. 1
Author(s):  
Marie E. P. Didier ◽  
Carlos Macias-Romero ◽  
Claire Teulon ◽  
Pascal Jourdain ◽  
Sylvie Roke
Keyword(s):  
Real Time ◽  
Second Harmonic ◽  
Morphological Changes ◽  
Harmonic Imaging ◽  
Wide Field ◽  
Label Free ◽  
Neuronal Cytoskeleton

scholarly journals Advanced Biosensing towards Real-Time Imaging of Protein Secretion from Single Cells

2020 ◽  
Author(s):  
Lang Zhou ◽  
Pengyu Chen ◽  
Aleksandr Simonian
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Protein Secretion ◽  
Intercellular Communication ◽  
Single Cells ◽  
Bone Marrow Failure ◽  
Cellular Protein ◽  
Label Free ◽  
Immune Effector ◽  
Real Time Imaging

Protein secretion of cells plays a vital role in intercellular communication. The abnormality and dysfunction of cellular protein secretion are associated with various physiological disorders, such as malignant proliferation of cells, aberrant immune function, and bone marrow failure. The heterogeneity of protein secretion exists not only between varying populations of cells, but also in the same phenotype of cells. Therefore, characterization of protein secretion from single cell contributes not only to the understanding of intercellular communication in immune effector, carcinogenesis and metastasis, but also to the development and improvement of diagnosis and therapy of relative diseases. In spite of abundant highly sensitive methods that have been developed for the detection of secreted proteins, majority of them fall short in providing sufficient spatial and temporal resolution for comprehensive profiling of protein secretion from single cells. The real-time imaging techniques allow rapid acquisition and manipulation of analyte information on a 2D plane, providing high spatiotemporal resolution. Here, we summarize recent advances in real-time imaging of secretory proteins from single cell, including label-free and labelling techniques, shedding light on the development of simple yet powerful methodology for real-time imaging of single-cell protein secretion.

scholarly journals Real-Time Multidepth Multiphoton Microscopy Using Pixel-to-Pixel Focus-Switching

2020 ◽  
Vol 10 (20) ◽  
pp. 7173
Author(s):  
Yifan Qin ◽  
Deying Chen ◽  
Yuanqin Xia
Keyword(s):  
Real Time ◽  
Imaging System ◽  
Second Harmonic ◽  
Multiphoton Microscopy ◽  
Cost Effective ◽  
Axial Direction ◽  
Three Dimensions ◽  
Mouse Lung ◽  
Label Free

Multiphoton microscopy is a well-established technique for biomedical applications, but real-time multidepth multimodal multiphoton microscopy using non-imaging detection has barely been discussed. We demonstrate a novel label-free imaging system capable of generating multimodal multiphoton signals at different focal planes simultaneously. Two spatially overlapped and temporally interlaced beams are obtained by applying cost-effective electro-optic modulator (EOM)-based fast-switching light paths. The switching beams have different divergence properties, enabling imaging at different depths into samples. The EOM is synchronized to the pixel clock from the microscope, achieving pixel-to-pixel focus-switching. The capability of the imaging system is demonstrated by performing real-time multidepth two-photon fluorescence (TPF) and second-harmonic generation (SHG) imaging of freshly excised mouse lung lobes. TPF and SHG images are acquired at two wavelength ranges. One is between 415 and 455 nm, and the other is between 495 and 635 nm. The microenvironment of pulmonary alveoli is depicted by the distributions of both elastin fibers visualized by TPF and collagen fibers illustrated by SHG. Macrophages residing inside apparent alveolar lumens are also identified by TPF, which shows that the imaging system is capable of localizing biological objects in three dimensions and has the potential of monitoring in vivo cellular dynamics in the axial direction.

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