Mapping membrane-potential perturbations of chromaffin cells exposed to electric fields

2002 ◽  
Vol 30 (4) ◽  
pp. 1516-1524 ◽  
Author(s):  
N. Hassan ◽  
I. Chatterjee ◽  
N.G. Publicover ◽  
G.L. Craviso
Keyword(s):  
Membrane Potential ◽  
Electric Fields ◽  
Chromaffin Cells

scholarly journals Synaptobrevin-2 C-terminal Flexible Region Regulates the Discharge of Catecholamine through the Fusion Pore

2018 ◽  
Author(s):  
Annita N. Weiss
Keyword(s):  
Membrane Potential ◽  
Lipid Bilayer ◽  
Chromaffin Cells ◽  
Pore Formation ◽  
Transmembrane Domain ◽  
Flash Photolysis ◽  
Fusion Pore ◽  
Snare Protein ◽  
Head Groups ◽  
Flexible Region

AbstractThe discharge of neurotransmitters from vesicles is a regulated process. Synaptobrevin-2 a SNARE protein, participates in this process through its interaction with other SNARE and associate proteins. Synaptobrevin-2 transmembrane domain is embedded into the vesicle lipid bilayer except for its last three residues. These residues are hydrophilic and constitute synaptobrevin-2 C-terminal flexible region. This region interacts with the intravesicular lipid bilayer phosphate head groups to initiate the fusion pore formation. Here it is shown that, this region also modulates the intravesicular membrane potential thereby the discharged of catecholamine. Synapotobrevin-2 Y113 residue was mutated to lysine or glutamate. The effects of these mutations on the exocytotic process in chromaffin cells were assessed using capacitance measurements, combined with amperometry and stimulation by flash photolysis of caged Ca2+. Both Y113E and Y113K mutations reduced the amplitudes of vesicle fusions and reduced the rates of release of catecholamine molecules in quanta release events. Further investigation revealed that the proximity of these charged residues near the vesicle lipid bilayer most likely changed the intravesicular potential, thereby slowing the flux of ions through the fusion pore, hence reducing the rate of catecholamine secretion. These results suggest that catecholamine efflux is couple with the intravesicular membrane potential.

scholarly journals Feasibility analysis of semiconductor voltage nanosensors for neuronal membrane potential sensing

2019 ◽  
Author(s):  
Anastasia Ludwig ◽  
Pablo Serna ◽  
Lion Morgenstein ◽  
Gaoling Yang ◽  
Omri Bar-Elli ◽  
...  
Keyword(s):  
Membrane Potential ◽  
Electric Fields ◽  
Stark Effect ◽  
High Sensitivity ◽  
Time Lapse ◽  
Neuronal Membrane ◽  
Attractive Alternative ◽  
Wide Field ◽  
Particle Level ◽  
Semiconductor Voltage

AbstractIn the last decade, optical imaging methods have significantly improved our understanding of the information processing principles in the brain. Although many promising tools have been designed, sensors of membrane potential are lagging behind the rest. Semiconductor nanoparticles are an attractive alternative to classical voltage indicators, such as voltage-sensitive dyes and proteins. Such nanoparticles exhibit high sensitivity to external electric fields via the quantum-confined Stark effect. Here we report the development of lipid-coated semiconductor voltage-sensitive nanorods (vsNRs) that self-insert into the neuronal membrane. We describe a workflow to detect and process the photoluminescent signal of vsNRs after wide-field time-lapse recordings. We also present data indicating that vsNRs are feasible for sensing membrane potential in neurons at a single-particle level. This shows the potential of vsNRs for detection of neuronal activity with unprecedentedly high spatial and temporal resolution.

scholarly journals Veratridine-induced oscillations of cytosolic calcium and membrane potential in bovine chromaffin cells.

1995 ◽  
Vol 482 (1) ◽  
pp. 15-27 ◽  
Author(s):  
M G López ◽  
A R Artalejo ◽  
A G García ◽  
E Neher ◽  
J García-Sancho
Keyword(s):  
Membrane Potential ◽  
Chromaffin Cells ◽  
Cytosolic Calcium ◽  
Bovine Chromaffin Cells

Characteristics of Receptor-Operated and Membrane Potential-Dependent ATP Secretion from Adrenal Medullary Chromaffin Cells

1990 ◽  
Vol 603 (1 Biological Ac) ◽  
pp. 311-322 ◽  
Author(s):  
EDUARDO ROJAS ◽  
VALENTÍN CEÑA ◽  
ANDRES STUTZIN ◽  
ERIK FORSBERG ◽  
HARVEY B. POLLARD
Keyword(s):  
Membrane Potential ◽  
Chromaffin Cells ◽  
Atp Secretion
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