Variable Ramp Rate Breakdown Experiments and the Role of Metal Injection in Low- $k$ Dielectrics

2011 ◽  
Vol 58 (12) ◽  
pp. 4354-4360 ◽  
Author(s):  
Joel L. Plawsky ◽  
Juan Borja ◽  
Brian Williams ◽  
Michael J. Riley ◽  
William N. Gill
Keyword(s):  
Ramp Rate ◽  
Low K

scholarly journals Moisture Induced Degradation of Porous Low-k Materials

2006 ◽  
Vol 914 ◽  
Author(s):  
Mikhail Baklanov ◽  
David O'Dwyer ◽  
Adam M Urbanowicz ◽  
Quoc Toan Le ◽  
Steven Demuynck ◽  
...  
Keyword(s):  
Water Film ◽  
Internal Surface ◽  
Hydrophobic Surface ◽  
Adsorbed Water ◽  
Bulk Water ◽  
Oh Groups ◽  
Film Bulk ◽  
Low K

AbstractInteraction of moisture with porous low-k films is evaluated by using in situ ellipsometry setup. The adsorbed water amount is calculated from change of refractive index measured during the adsorption. Pristine low-k films reversibly adsorb 2 - 5% of water that reflects presence of constitutive hydrophilic centrums. Plasma and thermal treatments increase the number of hydrophilic centrums. Once the amount of these centrums has reached a certain critical value sufficient to form a continuous water film, bulk water condensation is observed. Change of properties during the water adsorption in the damaged films is not fully reversible. Each additional adsorption cycle increases the dielectric function of the film because of decreasing porosity, increasing skeleton density and shrinkage. The pressure corresponding to the bulk condensation allows us to calculate internal contact angle (internal surface energy) of low-k materials. The water molecules adsorbed on separate OH groups play the role of a catalyst that hydrolyses the siloxane bridges initially present on hydrophobic surface.

scholarly journals miR168 targets Argonaute1A mediated miRNAs regulation pathways in response to potassium deficiency stress in tomato

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Xin Liu ◽  
Chunchang Tan ◽  
Xin Cheng ◽  
Xiaoming Zhao ◽  
Tianlai Li ◽  
...  
Keyword(s):  
Root Growth ◽  
Transgenic Tomato ◽  
Integrated Analysis ◽  
Regulation Mechanism ◽  
Sequencing Analysis ◽  
Growth Modulation ◽  
Tomato Plants ◽  
Transgenic Tomato Plants ◽  
Low K

Abstract Background Potassium (K+) is an essential ion for most plants, as it is involved in the regulation of growth and development. K+ homeostasis in plant cells has evolved to facilitate plant adaptation to K+-deficiency stress. Argonaute1 (AGO1) is regulated by miR168 to modulate the small RNA regulatory pathway by RNA silencing complex (RISC) in tomatoes. However, the role of miR168-mediated regulation of AGO1 in the context of K+ deficiency stress in tomatoes has not been elucidated yet. Results SlmiR168 and its target gene SlAGO1A were differentially expressed among low-K+-tolerant JZ34 and low-K+-sensitive JZ18 tomato plants. Transgenic tomato plants constitutively expressing pri-SlmiR168a showed stronger root system growth, better leaves development, and higher K+ contents in roots under K+-deficiency stress than those of the transgenic tomato lines expressing rSlAGO1A (SlmiR168-resistant) and the wild type (WT). Deep sequencing analysis showed that 62 known microRNAs (miRNAs) were up-regulated in 35S:rSlAGO1 compared with WT tomatoes. The same miRNAs were down-regulated in 35S:SlmiR168a compared with WT plants. The integrated analysis found 12 miRNA/mRNA pairs from the 62 miRNAs, including the root growth and cytokinin (CTK)/abscisic acid (ABA) pathways. Conclusions The regulation mediated by SlmiR168 of SlAGO1A contributes to the plant development under low-K+ stress. Moreover, this regulation mechanism may influence downstream miRNA pathways in response to low-K+ stress through the CTK/ABA and root growth modulation pathways.

scholarly journals Aldosterone-dependent and -independent regulation of Na+ and K+ excretion and ENaC in mouse kidneys

2020 ◽  
Vol 319 (2) ◽  
pp. F323-F334
Author(s):  
Lei Yang ◽  
Gustavo Frindt ◽  
Yuanyuan Xu ◽  
Shinichi Uchida ◽  
Lawrence G. Palmer
Keyword(s):  
Glomerular Filtration Rate ◽  
Western Blot ◽  
Filtration Rate ◽  
Ex Vivo ◽  
Western Blots ◽  
High K ◽  
Collecting Ducts ◽  
Low K ◽  
Clearance Methods

We investigated the regulation of Na+ and K+ excretion and the epithelial Na+ channel (ENaC) in mice lacking the gene for aldosterone synthase (AS) using clearance methods to assess excretion and electrophysiology and Western blot analysis to test for ENaC activity and processing. After 1 day of dietary Na+ restriction, AS−/− mice lost more Na+ in the urine than AS+/+ mice did. After 1 wk on this diet, both genotypes strongly reduced urinary Na+ excretion, but creatinine clearance decreased only in AS−/− mice. Only AS+/+ animals exhibited increased ENaC function, assessed as amiloride-sensitive whole cell currents in collecting ducts or cleavage of αENaC and γENaC in Western blots. To assess the role of aldosterone in the excretion of a K+ load, animals were fasted overnight and refed with high-K+ or low-K+ diets for 5 h. Both AS+/+ and AS−/− mice excreted a large amount of K+ during this period. In both phenotypes the excretion was benzamil sensitive, indicating increased K+ secretion coupled to ENaC-dependent Na+ reabsorption. However, the increase in plasma K+ under these conditions was much larger in AS−/− animals than in AS+/+ animals. In both groups, cleavage of αENaC and γENaC increased. However, Na+ current measured ex vivo in connecting tubules was enhanced only in AS+/+ mice. We conclude that in the absence of aldosterone, mice can conserve Na+ without ENaC activation but at the expense of diminished glomerular filtration rate. Excretion of a K+ load can be accomplished through aldosterone-independent upregulation of ENaC, but aldosterone is required to excrete the excess K+ without hyperkalemia.

scholarly journals MicroRNA-194 (miR-194) regulates ROMK channel activity by targeting intersectin 1

2014 ◽  
Vol 306 (1) ◽  
pp. F53-F60 ◽  
Author(s):  
Dao-Hong Lin ◽  
Peng Yue ◽  
Chengbiao Zhang ◽  
Wen-Hui Wang
Keyword(s):  
Collecting Duct ◽  
Luciferase Reporter ◽  
Pcr Analysis ◽  
Seed Sequence ◽  
Cortical Collecting Duct ◽  
Channel Activity ◽  
Luciferase Reporter Gene ◽  
High K ◽  
Low K

The aim of the study is to explore the role of miR-194 in mediating the effect of high-K (HK) intake on ROMK channel. Northern blot analysis showed that miR-194 was expressed in kidney and that HK intake increased while low-K intake decreased the expression of miR-194. Real-time PCR analysis further demonstrated that HK intake increased the miR-194 expression in the cortical collecting duct. HK intake decreased the expression of intersectin 1 (ITSN1) which enhanced With-No-Lysine Kinase (WNK)-induced endocytosis of ROMK. Expression of miR-194 mimic decreased luciferase reporter gene activity in HEK293 T cells transfected with ITSN-1–3′UTR containing the complementary seed sequence for miR-194. In contrast, transfection of miR-194 inhibitor increased the luciferase activity. This effect was absent in the cells transfected with mutated 3′UTR of ITSN1 in which the complimentary seed sequence was deleted. Moreover, the inhibition of miR-194 expression increased the protein level of endogenous ITSN1 in HEK293T cells. Expression of miR-194 mimic also decreased the translation of exogenous ITSN1 in the cells transfected with the ITSN1 containing 3′UTR but not with 3′UTR-free ITSN1. Expression of pre-miR-194 increased K currents and ROMK expression in the plasma membrane in ROMK-transfected cells. Coexpression of ITSN1 reversed the stimulatory effect of miR-194 on ROMK channels. This effect was reversed by coexpression of ITSN1. We conclude that miR-194 regulates ROMK channel activity by modulating ITSN1 expression thereby enhancing ITSN1/WNK-dependent endocytosis. It is possible that miR-194 is involved in mediating the effect of a HK intake on ROMK channel activity.

scholarly journals Dependence of mammalian putrescine and spermidine transport on plasma-membrane potential: identification of an amiloride binding site on the putrescine carrier

1998 ◽  
Vol 330 (3) ◽  
pp. 1283-1291 ◽  
Author(s):  
Richard POULIN ◽  
Chenqi ZHAO ◽  
Savita VERMA ◽  
René CHAREST-GAUDREAULT ◽  
Marie AUDETTE
Keyword(s):  
Plasma Membrane ◽  
Membrane Potential ◽  
Binding Site ◽  
Biochemical Properties ◽  
Mitochondrial Potential ◽  
Plasma Membrane Potential ◽  
High K ◽  
Polyamine Transport ◽  
Low K

The mechanism of mammalian polyamine transport is poorly understood. We have investigated the role of plasma-membrane potential (ΔΨpm) in putrescine and spermidine uptake in ZR-75-1 human breast cancer cells. The rate of [3H]putrescine and [3H]spermidine uptake was inversely correlated to extracellular [K+] ([K+]o) and to ΔΨpm, as determined by the accumulation of [3H]tetraphenylphosphonium bromide (TPP). Inward transport was unaffected by a selective decrease in mitochondrial potential (ΔΨmit) induced by valinomycin at low [K+]o, but was reduced by ≈ 60% by the rheogenic protonophore carbonylcyanide m-chlorophenylhydrazone (CCCP), which rapidly (≤ 15 min) collapsed both ΔΨpm and ΔΨmit. Plasma-membrane depolarization by high [K+]o or CCCP did not enhance putrescine efflux in cells pre-loaded with [3H]putrescine, suggesting that decreased uptake caused by these agents did not result from a higher excretion rate. On the other hand, the electroneutral K+/H+ exchanger nigericin (10 μM) co-operatively depressed [3H]TPP, [3H]putrescine and [3H]spermidine uptake in the presence of ouabain. Suppression of putrescine uptake by nigericin+ouabain was Na+-dependent, suggesting that plasma-membrane repolarization by the electrogenic Na+ pump was required upon acidification induced by nigericin, due to the activation of the Na+/H+ antiporter. The sole addition of 5-N,N-hexamethylene amiloride, a potent inhibitor of the Na+/H+ antiporter, strongly inhibited putrescine uptake in a competitive fashion [Ki 4.0±0.9 (S.D.) μM], while being a weaker antagonist of spermidine uptake. The potency of a series of amiloride analogues to inhibit putrescine uptake was clearly different from that of the Na+/H+ antiporter, and resembled that noted for Na+ co-transport proteins. These data demonstrate that putrescine and spermidine influx is mainly unidirectional and strictly depends on ΔΨpm, but not ΔΨmit. This report also provides first evidence for a high-affinity amiloride-binding site on the putrescine carrier, which provides new insight into the biochemical properties of this transporter.

The Critical Role of Dielectric Trench Etch in Enhancing Cu/low-k Dielectric Reliability for Advanced CMOS Technologies

2019 ◽  
Vol 44 (1) ◽  
pp. 357-361
Author(s):  
Jun-Qing Zhou ◽  
Min-Da Hu ◽  
Hai-Yang Zhang ◽  
Dong-Jiang Wang ◽  
Xin-Peng Wang ◽  
...  
Keyword(s):  
Critical Role ◽  
Low K

scholarly journals Role of SPAK in Mediating Regulation of NCC in the Kidney in Response to Low K Diet

2012 ◽  
Vol 26 (S1) ◽  
Author(s):  
Jie Liu ◽  
Richard A. Coleman ◽  
P. Richard Grimm ◽  
Eric Delpire ◽  
Paul A. Welling ◽  
...  
Keyword(s):  
Low K

Rheologically determined phase diagram of poly(ε‐caprolactone)/poly(styrene‐ co ‐acrylonitrile) blends: Role of ramp rate in dynamic measurements

2019 ◽  
Vol 136 (28) ◽  
pp. 47750 ◽  
Author(s):  
Amir Ali Naziri ◽  
Hossein Ali Khonakdar ◽  
Farkhondeh Hemmati ◽  
Seyed Hassan Jafari ◽  
Morteza Ehsani
Keyword(s):  
Phase Diagram ◽  
Dynamic Measurements ◽  
Ramp Rate

Paleoarchean crustal evolution of the Singhbhum Craton, eastern India: Insights from granitoid petrology and zircon U-Pb and Lu-Hf systematics

2020 ◽  
Author(s):  
Aniruddha Mitra ◽  
Sukanta Dey ◽  
Keqing Zong ◽  
Yongsheng Liu ◽  
Anirban Mitra
Keyword(s):  
Stability Field ◽  
Eastern India ◽  
Singhbhum Craton ◽  
Whole Process ◽  
Sequence Of Events ◽  
High Silica ◽  
Significant Mechanism ◽  
Garnet Stability Field ◽  
Low K

<p>Singhbhum Craton, eastern India, exposes some of the oldest known composite Paleoarchean granitoids. These granitoids range from sodic TTGs to evolved, potassic granites.  The whole process of their formation, starting from nucleation of a juvenile continent to its evolution and final stabilization is documented. The central part of the craton started nucleating with the formation of 3.45–3.40Ga juvenile (zircon εHf<sub>t</sub>=+0.6 to +7.1) TTGs. These TTGs characterized by slightly depleted HREE and Y, negligible Eu-anomaly (Eu/Eu*=0.90 to 1.00) and moderate Sr/Y (25–64), consistent with derivation from a low-K mafic crust at a pressure near the lower end of the garnet stability field, causing subordinate garnet retention in the residue and negligible role of plagioclase. During 3.32Ga, deeper melting of a juvenile mafic crust (zircon εHf<sub>t</sub>=+1.3 to +5.7) caused emplacement of a second generation of TTG. Deeper melting is suggested by depleted HREE and Y, and high Sr/Y (52–155), implying significant amount of residual garnet retention. Subsequently at 3.28 and 3.25Ga, melting of moderately old to juvenile (zircon εHf<sub>t</sub>=-1.9 to +4.5), mostly TTG sources at variable depths generated potassic, LILE-enriched, high-silica granites. Intrusion of these potassic granites resulted in a stable and buoyant crust that marked the final Cratonization of the Singhbhum Craton. The sequence of events is interpreted in terms of repeated intracrustal melting and granitoid generation in a gradually thickening oceanic plateau with a progressive change in granitoid source from mafic to felsic in composition. Combination of rock assemblage, regional geology, and structural pattern also supports intraplate nature of the magmatism in Singhbhum Craton, which might have been a significant mechanism of crustal growth worldwide during Paleoarchean.</p>

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