Quantitative Analysis of Live-Cell Growth at the Shoot Apex of Arabidopsis thaliana: Algorithms for Feature Measurement and Temporal Alignment

2013 ◽  
Vol 10 (5) ◽  
pp. 1150-1161 ◽  
Author(s):  
Oben M. Tataw ◽  
G. Venugopala Reddy ◽  
Eamonn J. Keogh ◽  
Amit K. Roy-Chowdhury
Keyword(s):  
Arabidopsis Thaliana ◽  
Quantitative Analysis ◽  
Cell Growth ◽  
Shoot Apex ◽  
Live Cell ◽  
Temporal Alignment ◽  
Feature Measurement

Quantitative analysis of herbivore-induced cytosolic calcium by using a Cameleon (YC 3.6) calcium sensor in Arabidopsis thaliana

2014 ◽  
Vol 171 (2) ◽  
pp. 136-139 ◽  
Author(s):  
Francesca Verrillo ◽  
Andrea Occhipinti ◽  
Chidananda Nagamangala Kanchiswamy ◽  
Massimo E. Maffei
Keyword(s):  
Arabidopsis Thaliana ◽  
Quantitative Analysis ◽  
Cytosolic Calcium ◽  
Calcium Sensor

scholarly journals KNOX HOMOLOGS SHOOT MERISTEMLESS (STM) and KNAT6 are epistatic to CLAVATA3 (CLV3) during embryonic meristem development in Arabidopsis thaliana

2020 ◽  
Author(s):  
Sharma Nidhi ◽  
Liu Tie
Keyword(s):  
Arabidopsis Thaliana ◽  
Shoot Apex ◽  
Double Mutant ◽  
Genetic Interaction ◽  
Homeobox Gene ◽  
The Other ◽  
Shoot Meristem ◽  
Published Data ◽  
Meristem Development ◽  
Shoot Meristemless

AbstractIn Arabidopsis, the genes SHOOT MERISTEMLESS (STM) and CLAVATA3 (CLV3) antagonistically regulate shoot meristem development. STM is essential for both development and maintenance of the meristem, as stm mutants fail to develop a shoot meristem during embryogenesis. CLV3, on the other hand, negatively regulates meristem proliferation, and clv3 mutants possess an enlarged shoot meristem. Genetic interaction studies revealed that stm and clv3 dominantly suppress each other’s phenotypes. STM works in conjunction with its closely related homologue KNOTTED1-LIKE HOMEOBOX GENE 6 (KNAT6) to promote meristem development and organ separation, as stm knat6 double mutants fail to form a meristem and produce a fused cotyledon. In this study, we show that clv3 fails to promote post-embryonic meristem formation in stm-1 background if we also remove KNAT6. stm-1 knat6 clv3 triple mutants result in early meristem termination and produce fused cotyledons similar to stm knat6 double mutant. Notably, the stm-1 knat6 and stm-1 knat6 clv3 alleles lack tissue in the presumed region of SAM. stm knat6 clv3 also showed reduced inflorescence size and shoot apex size as compared to clv3 single or stm clv3 double mutants. In contrast to previously published data, these data suggest that stm is epistatic to clv3 in postembryonic meristem development.HighlightSTM and KNAT6 genes determine post-embryonic meristem formation and activity in Arabidopsis. clv3 mutation is unable to rescue the stm knat6 meristemless phenotype.

scholarly journals Live-cell Imaging and Quantitative Analysis of Meiotic Divisions in Caenorhabditis elegans Males

BIO-PROTOCOL ◽  
2020 ◽  
Vol 10 (20) ◽  
Author(s):  
Gunar Fabig ◽  
Falko Löffler ◽  
Christian Götze ◽  
Thomas Müller-Reichert
Keyword(s):  
Quantitative Analysis ◽  
Caenorhabditis Elegans ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell

Live-Cell Imaging of F-Actin Dynamics During Fertilization in Arabidopsis thaliana

2017 ◽  
pp. 47-54 ◽  
Author(s):  
Daichi Susaki ◽  
Daisuke Maruyama ◽  
Ramesh Yelagandula ◽  
Frederic Berger ◽  
Tomokazu Kawashima
Keyword(s):  
Arabidopsis Thaliana ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Actin Dynamics
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