scholarly journals Live-cell Imaging and Quantitative Analysis of Embryonic Epithelial Cells in Xenopus laevis

10.3791/1949 ◽  
2010 ◽  
Author(s):  
Sagar D. Joshi ◽  
Lance A. Davidson
Keyword(s):  
Quantitative Analysis ◽  
Epithelial Cells ◽  
Xenopus Laevis ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell

scholarly journals Live-cell Imaging and Quantitative Analysis of Meiotic Divisions in Caenorhabditis elegans Males

BIO-PROTOCOL ◽  
2020 ◽  
Vol 10 (20) ◽  
Author(s):  
Gunar Fabig ◽  
Falko Löffler ◽  
Christian Götze ◽  
Thomas Müller-Reichert
Keyword(s):  
Quantitative Analysis ◽  
Caenorhabditis Elegans ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell

scholarly journals Deep Learning Automates the Quantitative Analysis of Individual Cells in Live-Cell Imaging Experiments

2016 ◽  
Vol 12 (11) ◽  
pp. e1005177 ◽  
Author(s):  
David A. Van Valen ◽  
Takamasa Kudo ◽  
Keara M. Lane ◽  
Derek N. Macklin ◽  
Nicolas T. Quach ◽  
...  
Keyword(s):  
Deep Learning ◽  
Quantitative Analysis ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell

scholarly journals Live cell imaging reveals focal adhesions mechanoresponses in mammary epithelial cells under sustained equibiaxial stress

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Lorena Sigaut ◽  
Catalina von Bilderling ◽  
Micaela Bianchi ◽  
Juan Eduardo Burdisso ◽  
Laura Gastaldi ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Mammary Epithelial Cells ◽  
Focal Adhesions ◽  
Mammary Epithelial ◽  
Live Cell

scholarly journals Quantitative live cell imaging of nanoparticle translocation into/across lung alveolar epithelial cells

2012 ◽  
Vol 26 (S1) ◽  
Author(s):  
Arnold Sipos ◽  
Farnoosh Fazlollahi ◽  
Yong Ho Kim ◽  
Robert H. Chow ◽  
Zea Borok ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Alveolar Epithelial Cells ◽  
Alveolar Epithelial

scholarly journals Morphodynamical cell-state description via live-cell imaging trajectory embedding

2021 ◽  
Author(s):  
Jeremy Copperman ◽  
Sean M Gross ◽  
Young Hwan Chang ◽  
Laura M Heiser ◽  
Daniel M Zuckerman
Keyword(s):  
Quantitative Analysis ◽  
Morphological Feature ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Morphological Changes ◽  
Live Cell ◽  
State Transitions ◽  
Dynamical Response ◽  
Cell State ◽  
Descriptive Models

Time-lapse imaging provides powerful insight into the dynamical response of cells to perturbation, but the quantitative analysis of morphological changes over time is a challenge. Here, we exploit the concept of "morphodynamical trajectories" to analyze cellular behavior using morphological feature trajectory histories, rather than the common practice of examining morphological feature time courses in the space of single-timepoint (snapshot) morphological features. Our morphodynamical trajectory embedding analysis yielded quantitative and descriptive models of future time points based on the extended history information of MCF10A mammary epithelial cells treated with a panel of ligands. The trajectory analysis constructs a shared morphodynamical cell-state landscape, where the response of MCF10A cells induced by various extracellular signals is characterized by ligand-specific regulation of state transitions. Additionally, we show that including trajectories in single-cell morphological analysis enables (i) systematic characterization of cell state trajectories, and (ii) better separation of phenotypes and more descriptive models of ligand-induced differences as compared to snapshot-based analysis. This morphodynamical trajectory embedding is broadly applicable for the quantitative analysis of cell responses via live-cell imaging across many biological and biomedical applications.

scholarly journals Vectorial insertion of apical and basolateral membrane proteins in polarized epithelial cells revealed by quantitative 3D live cell imaging

2006 ◽  
Vol 172 (7) ◽  
pp. 1035-1044 ◽  
Author(s):  
Wei Hua ◽  
David Sheff ◽  
Derek Toomre ◽  
Ira Mellman
Keyword(s):  
Membrane Proteins ◽  
Epithelial Cells ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fluorescent Protein ◽  
Yellow Fluorescent Protein ◽  
Basolateral Membrane ◽  
Live Cell ◽  
Junctional Complex ◽  
Apical Surface

Although epithelial cells are known to exhibit a polarized distribution of membrane components, the pathways responsible for delivering membrane proteins to their appropriate domains remain unclear. Using an optimized approach to three-dimensional live cell imaging, we have visualized the transport of newly synthesized apical and basolateral membrane proteins in fully polarized filter-grown Madin–Darby canine kidney cells. We performed a detailed quantitative kinetic analysis of trans-Golgi network (TGN) exit, passage through transport intermediates, and arrival at the plasma membrane using cyan/yellow fluorescent protein–tagged glycosylphosphatidylinositol-anchored protein and vesicular stomatitis virus glycoprotein as apical and basolateral reporters, respectively. For both pathways, exit from the TGN was rate limiting. Furthermore, apical and basolateral proteins were targeted directly to their respective membranes, resolving current confusion as to whether sorting occurs on the secretory pathway or only after endocytosis. However, a transcytotic protein did reach the apical surface after a prior appearance basolaterally. Finally, newly synthesized proteins appeared to be delivered to the entire lateral or apical surface, suggesting—contrary to expectations—that there is not a restricted site for vesicle docking or fusion adjacent to the junctional complex.

Live-cell imaging microscopy and quantitative analysis of Ca2+-dependent effects of neurotransmitters on DNA in snail neurons

BIOPHYSICS ◽  
2014 ◽  
Vol 59 (1) ◽  
pp. 91-97 ◽  
Author(s):  
A. Kh. Timoshenko ◽  
A. V. Shevelkin ◽  
V. P. Nikitin ◽  
V. V. Sherstnev
Keyword(s):  
Quantitative Analysis ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Snail Neurons
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