Robotic Immobilization of Motile Sperm for Clinical Intracytoplasmic Sperm Injection

2019 ◽  
Vol 66 (2) ◽  
pp. 444-452 ◽  
Author(s):  
Zhuoran Zhang ◽  
Changsheng Dai ◽  
James Huang ◽  
Xian Wang ◽  
Jun Liu ◽  
...  
Keyword(s):  
Intracytoplasmic Sperm Injection ◽  
Motile Sperm

scholarly journals FertDish: microfluidic sperm selection-in-a-dish for intracytoplasmic sperm injection

Lab on a Chip ◽  
2021 ◽  
Vol 21 (4) ◽  
pp. 775-783
Author(s):  
Sa Xiao ◽  
Jason Riordon ◽  
Mohammad Simchi ◽  
Alexander Lagunov ◽  
Thomas Hannam ◽  
...  
Keyword(s):  
Intracytoplasmic Sperm Injection ◽  
Motile Sperm ◽  
Sperm Selection ◽  
High Recovery ◽  
Dna Quality

The FertDish features a clinically applicable sperm processing format, and enables high recovery of motile sperm with high DNA quality.

Fertilizing capacity of epididymal and testicular sperm using intracytoplasmic sperm injection (ICSI)

1995 ◽  
Vol 7 (2) ◽  
pp. 281 ◽  
Author(s):  
SJ Silber ◽  
P Devroey ◽  
H Tournaye ◽  
Steirteghem AC Van
Keyword(s):  
Pregnancy Rate ◽  
Intracytoplasmic Sperm Injection ◽  
Pregnancy Rates ◽  
Obstructive Azoospermia ◽  
Motile Sperm ◽  
Epididymal Sperm ◽  
Testicular Sperm ◽  
Cleavage Rate

For men with uncorrectable obstructive azoospermia, their only hope of fathering a child is microsurgical epididymal sperm aspiration (MESA) combined with in vitro fertilization (IVF). In 1988, proximal epididymal sperm were demonstrated to have better motility than senescent sperm in the distal epididymis, and it was thought that retrieval of motile sperm from the proximal epididymis would yield reliable fertilization and pregnancy rates after conventional IVF. However, the results to date have been poor, and although a minority of patients achieved good fertilization rates with IVF, the vast majority (81%) had consistently poor or no fertilization and the pregnancy rate averaged only 9%. Recently, intracytoplasmic sperm injection (ICSI) has been successfully used to achieve fertilization and pregnancies for patients with extreme oligoasthenozoospermia. ICSI has therefore been applied to cases of obstructive azoospermia and, in this report, 67 MESA-IVF cases are compared with 72 MESA-ICSI cases. The principle that motile sperm from the proximal segments of the epididymis should be used for ICSI was followed, although in the most severe cases in which there was an absence of the epididymis (or absence of sperm in the epididymis), testicular sperm were obtained from macerated testicular biopsies. These sperm only exhibited a weak, twitching motion. In 72 consecutive MESA cases, ICSI resulted in fertilization and normal embryos for transfer in 90% of the cases, with an overall fertilization rate of 46%, a cleavage rate of 68%, and ongoing or delivered pregnancy rates of 46% per transfer and 42% per cycle. The pregnancy and take-home baby rates increased from 9% and 4.5% with IVF to 53% and 42% with ICSI. There were no differences between the results for fresh epididymal, frozen epididymal or testicular sperm, and the number of eggs collected did not affect the outcome. The results were also unaffected by the aetiology of the obstruction such as congenital absence of the vas deferens or failed vasoepididymostomy. The only significant factor which affected the pregnancy rate was female age. It is concluded that although complex mechanisms involving epididymal transport may be beneficial for conventional fertilization of human oocytes (in vivo or in vitro), none of these mechanisms are required for fertilization after ICSI. Given the excellent results with epididymal and testicular sperm, ICSI is obligatory for all future MESA patients. Finally, the use of ICSI with testicular sperm from men with non-obstructive azoospermia is also discussed.

EFFECTIVENESS OF ASSISTED REPRODUCTIVE TREATMENT PROGRAMS DEPENDING ON THE CHARACTERISTICS OF SPERMOGRAM CHANGES

2018 ◽  
Vol 19 (2) ◽  
pp. 82-87 ◽  
Author(s):  
S. I. Gamidov ◽  
R. I. Ovchinnikov ◽  
A. Yu. Popova ◽  
V. V. Polozov ◽  
N. P. Naumov ◽  
...  
Keyword(s):  
Intracytoplasmic Sperm Injection ◽  
Assisted Reproductive Technologies ◽  
Sperm Concentration ◽  
Reproductive Technologies ◽  
Motile Sperm ◽  
Study Objective ◽  
Normal Morphology ◽  
Live Births ◽  
Significant Difference

Introduction. There’re some pathological mechanisms of male fertility disorders that still don’t have proper diagnostic tests. This significantly decreases diagnostic value of a spermogram and makes the problem of evaluation of the characteristics of spermogram changes and their effects on the effectiveness of assisted reproductive technologies (ART) a pressing problem.The study objectiveis to identify the correlation between effectiveness of ART programs and the characteristics of spermogram changes, in particular sperm concentration, motility, and morphology Materials and methods. At the V.I. Kulakov Research Center for Obstetrics, Gynecology and Perinatology in the period from December of 2012 to December of 2016, 10,042 married couples who underwent treatment using ART (2221 – in vitro insemination (IVF), 7821 – IVF with intracytoplasmic sperm injection) were examined.Results. In patients after IVF, the frequency of live births significantly depended on sperm concentration: 28.6 % for concentration above 5 mil/ml and 51.5 % for concentration above 15 mil/ml (p <0.0001). No significant difference was observed for the dependence of the frequency of live births on the number of progressive-motile sperm (grade А): 38.2 % for <5 % and 57.7 % for >15 % (p = 0.11), or on the number of spermatozoa with normal morphology: 50 % for ≥4 % and 45.5 % for <4 % (p = 0,23). In patients after IVF with intracytoplasmic sperm injection, the concentration of spermatozoa, number of progressive-motile sperm (grade А), and number of spermatozoa with normal morphology didn’t affect the frequency of live births in a statistically significant way.Conclusion. Sperm concentration, motility, and morphology can affect the frequency of live births in the IVF program, but statistically significant correlation was observed only for sperm concentration. After IVF with intracytoplasmic sperm injection, only sperm morphology affects the frequency of live births, but not in a statistically significant way. 

Total motile sperm count has a superior predictive value over the WHO 2010 cut-off values for the outcomes of intracytoplasmic sperm injection cycles

Andrology ◽  
2016 ◽  
Vol 4 (5) ◽  
pp. 880-886 ◽  
Author(s):  
E. Borges ◽  
A. S. Setti ◽  
D. P. A. F. Braga ◽  
R. C. S. Figueira ◽  
A. Iaconelli
Keyword(s):  
Intracytoplasmic Sperm Injection ◽  
Predictive Value ◽  
Sperm Count ◽  
Motile Sperm ◽  
Total Motile Sperm Count

371 INTRACYTOPLASMIC SPERM INJECTION OF EQUINE OOCYTES USING AIR-DRIED SPERM OR SPERM STORED IN A HIGH OSMOLARITY MEDIUM

2007 ◽  
Vol 19 (1) ◽  
pp. 301 ◽  
Author(s):  
A. Alonso ◽  
M. Miragaya ◽  
L. Losinno ◽  
C. Herrera
Keyword(s):  
Growth Factor ◽  
Liquid Nitrogen ◽  
Intracytoplasmic Sperm Injection ◽  
Animal Health ◽  
Polar Body ◽  
Control Group ◽  
Motile Sperm ◽  
Cleavage Rate ◽  
High Osmolarity ◽  
Short Period

The generally accepted method of long-term sperm preservation is freezing in liquid nitrogen. However, it is not always available. Other techniques have shown to preserve sperm for a short period of time that can be used for intracytoplasmic sperm injection (ICSI). Mouse offspring have been produced after ICSI with sperm stored in a high osmolarity medium. Also, human embryos were obtained by ICSI with air-dried sperm. Recently, equine blastocysts have been obtained by ICSI with lyophilized sperm. In our study, cleavage rate was evaluated after ICSI of equine oocytes using air-dried sperm or sperm stored in a high osmolarity medium. Oocytes were obtained from slaughterhouse ovaries by scraping individual follicles and transported in a portable incubator at 38�C for 15 h in TCM-199 buffered with HEPES and supplemented with glutamine, sodium pyruvate, LH (Bioniche Animal Health, Inc., Beltville, Ontario, Canada), FSH (Bioniche Animal Health, Inc.), epidermal growth factor (EGF), insulin-like growth factor 1 (IGF-1), and 10% FBS (GIBCO, Grand Island, NY, USA). On arrival, they were cultured for 6 more hours in 5% CO2 in air at 38�C in microdroplets of the same medium, without HEPES. Sperm was collected from 2 stallions of proven fertility. The ejaculate was diluted in Kenney extender, centrifuged, and the pellet was resuspended in HEPES-TALP (H-TALP). Three sperm treatments were used: (C) Control: ejaculated motile sperm processed as described above; (1) Air-dried sperm: obtained from spreading sperm on a sterile slide and drying it for 10 min in a laminar flow chamber; (2) Sperm in a high osmolarity medium: ejaculated motile sperm resuspended in H-TALP with high osmolarity (800 mOsmol). Samples from groups 1 and 2 were stored at 5�C for 2 to 3 days before being used for sperm injection. Only oocytes with an intact cytoplasm and a visible polar body were selected for injection and randomly assigned to each experimental group. Each MII oocyte was injected with 1 sperm cell and activated in Ionomicin for 10 min and DMAP for 3 h. Injected oocytes were cultured in DMEM : F10 1 : 1 with 10% FBS at 38�C in 7% O2 and 5% CO2 for 48 h. The number of cleaved embryos was recorded. Data was analyzed by chi-square test. A total of 135 MII oocytes were injected. The cleavage rate in group 1 was significantly lower than in the control group (31/71, 43.66% vs. 28/38, 73.68%) (P &lt; 0.05). No differences were observed between group (2) and control (19/29, 73.07% vs. 28/38, 73.68%) (P &gt; 0.05). This is the first report of equine oocytes fertilized by ICSI with air-dried sperm or with sperm kept in high osmolarity medium. These simple sperm preservation techniques might be an alternative option when liquid nitrogen is not available. Further studies will determine if it is possible to obtain pregnancies or even healthy offspring.

1406: Testicular Sperm Extraction Combined with Intracytoplasmic Sperm Injection in Men with Postchemotherapy Azoospermia

2006 ◽  
Vol 175 (4S) ◽  
pp. 453-454
Author(s):  
Hiroshi Okada ◽  
Osamu Maruyama ◽  
Kojiro Nishio ◽  
Keisuke Saito ◽  
Takashi Yoshii ◽  
...  
Keyword(s):  
Intracytoplasmic Sperm Injection ◽  
Testicular Sperm Extraction ◽  
Testicular Sperm

1630: Sufficient Total Motile Sperm for Intrauterine Insemination is Easily Obtained by Assisted Ejaculation of Men with Spinal Cord Injury

2006 ◽  
Vol 175 (4S) ◽  
pp. 525-525
Author(s):  
Charles M. Lynne ◽  
Nancy L. Brackett ◽  
Teodoro C. Aballa ◽  
Emad Ibrahim ◽  
Apostolos Kafetsoulis
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Intrauterine Insemination ◽  
Motile Sperm ◽  
Cord Injury ◽  
Assisted Ejaculation
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