scholarly journals FertDish: microfluidic sperm selection-in-a-dish for intracytoplasmic sperm injection

Lab on a Chip ◽  
2021 ◽  
Vol 21 (4) ◽  
pp. 775-783
Author(s):  
Sa Xiao ◽  
Jason Riordon ◽  
Mohammad Simchi ◽  
Alexander Lagunov ◽  
Thomas Hannam ◽  
...  
Keyword(s):  
Intracytoplasmic Sperm Injection ◽  
Motile Sperm ◽  
Sperm Selection ◽  
High Recovery ◽  
Dna Quality

The FertDish features a clinically applicable sperm processing format, and enables high recovery of motile sperm with high DNA quality.

Fertilizing capacity of epididymal and testicular sperm using intracytoplasmic sperm injection (ICSI)

1995 ◽  
Vol 7 (2) ◽  
pp. 281 ◽  
Author(s):  
SJ Silber ◽  
P Devroey ◽  
H Tournaye ◽  
Steirteghem AC Van
Keyword(s):  
Pregnancy Rate ◽  
Intracytoplasmic Sperm Injection ◽  
Pregnancy Rates ◽  
Obstructive Azoospermia ◽  
Motile Sperm ◽  
Epididymal Sperm ◽  
Testicular Sperm ◽  
Cleavage Rate

For men with uncorrectable obstructive azoospermia, their only hope of fathering a child is microsurgical epididymal sperm aspiration (MESA) combined with in vitro fertilization (IVF). In 1988, proximal epididymal sperm were demonstrated to have better motility than senescent sperm in the distal epididymis, and it was thought that retrieval of motile sperm from the proximal epididymis would yield reliable fertilization and pregnancy rates after conventional IVF. However, the results to date have been poor, and although a minority of patients achieved good fertilization rates with IVF, the vast majority (81%) had consistently poor or no fertilization and the pregnancy rate averaged only 9%. Recently, intracytoplasmic sperm injection (ICSI) has been successfully used to achieve fertilization and pregnancies for patients with extreme oligoasthenozoospermia. ICSI has therefore been applied to cases of obstructive azoospermia and, in this report, 67 MESA-IVF cases are compared with 72 MESA-ICSI cases. The principle that motile sperm from the proximal segments of the epididymis should be used for ICSI was followed, although in the most severe cases in which there was an absence of the epididymis (or absence of sperm in the epididymis), testicular sperm were obtained from macerated testicular biopsies. These sperm only exhibited a weak, twitching motion. In 72 consecutive MESA cases, ICSI resulted in fertilization and normal embryos for transfer in 90% of the cases, with an overall fertilization rate of 46%, a cleavage rate of 68%, and ongoing or delivered pregnancy rates of 46% per transfer and 42% per cycle. The pregnancy and take-home baby rates increased from 9% and 4.5% with IVF to 53% and 42% with ICSI. There were no differences between the results for fresh epididymal, frozen epididymal or testicular sperm, and the number of eggs collected did not affect the outcome. The results were also unaffected by the aetiology of the obstruction such as congenital absence of the vas deferens or failed vasoepididymostomy. The only significant factor which affected the pregnancy rate was female age. It is concluded that although complex mechanisms involving epididymal transport may be beneficial for conventional fertilization of human oocytes (in vivo or in vitro), none of these mechanisms are required for fertilization after ICSI. Given the excellent results with epididymal and testicular sperm, ICSI is obligatory for all future MESA patients. Finally, the use of ICSI with testicular sperm from men with non-obstructive azoospermia is also discussed.

Sperm telomere length in motile sperm selection techniques: A qFISH approach

Andrologia ◽  
2017 ◽  
Vol 50 (2) ◽  
pp. e12840 ◽  
Author(s):  
R. Lafuente ◽  
E. Bosch-Rue ◽  
J. Ribas-Maynou ◽  
J. Alvarez ◽  
C. Brassesco ◽  
...  
Keyword(s):  
Telomere Length ◽  
Motile Sperm ◽  
Sperm Selection ◽  
Sperm Telomere Length

scholarly journals Comparison of Intracytoplasmic Sperm Injection Outcome with Sperm Selection Techniques in Oligoasthenozoospermic Males: A Randomized Controlled Trial

2019 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Maryam Sefidgar Tehrani ◽  
Malihe Amirian ◽  
Mohsen Jalali ◽  
Armin Attaranzadeh ◽  
Alireza Fazel ◽  
...  
Keyword(s):  
Randomized Controlled Trial ◽  
Intracytoplasmic Sperm Injection ◽  
Controlled Trial ◽  
Sperm Selection ◽  
Randomized Controlled

Robotic Immobilization of Motile Sperm for Clinical Intracytoplasmic Sperm Injection

2019 ◽  
Vol 66 (2) ◽  
pp. 444-452 ◽  
Author(s):  
Zhuoran Zhang ◽  
Changsheng Dai ◽  
James Huang ◽  
Xian Wang ◽  
Jun Liu ◽  
...  
Keyword(s):  
Intracytoplasmic Sperm Injection ◽  
Motile Sperm

EFFECTIVENESS OF ASSISTED REPRODUCTIVE TREATMENT PROGRAMS DEPENDING ON THE CHARACTERISTICS OF SPERMOGRAM CHANGES

2018 ◽  
Vol 19 (2) ◽  
pp. 82-87 ◽  
Author(s):  
S. I. Gamidov ◽  
R. I. Ovchinnikov ◽  
A. Yu. Popova ◽  
V. V. Polozov ◽  
N. P. Naumov ◽  
...  
Keyword(s):  
Intracytoplasmic Sperm Injection ◽  
Assisted Reproductive Technologies ◽  
Sperm Concentration ◽  
Reproductive Technologies ◽  
Motile Sperm ◽  
Study Objective ◽  
Normal Morphology ◽  
Live Births ◽  
Significant Difference

Introduction. There’re some pathological mechanisms of male fertility disorders that still don’t have proper diagnostic tests. This significantly decreases diagnostic value of a spermogram and makes the problem of evaluation of the characteristics of spermogram changes and their effects on the effectiveness of assisted reproductive technologies (ART) a pressing problem.The study objectiveis to identify the correlation between effectiveness of ART programs and the characteristics of spermogram changes, in particular sperm concentration, motility, and morphology Materials and methods. At the V.I. Kulakov Research Center for Obstetrics, Gynecology and Perinatology in the period from December of 2012 to December of 2016, 10,042 married couples who underwent treatment using ART (2221 – in vitro insemination (IVF), 7821 – IVF with intracytoplasmic sperm injection) were examined.Results. In patients after IVF, the frequency of live births significantly depended on sperm concentration: 28.6 % for concentration above 5 mil/ml and 51.5 % for concentration above 15 mil/ml (p <0.0001). No significant difference was observed for the dependence of the frequency of live births on the number of progressive-motile sperm (grade А): 38.2 % for <5 % and 57.7 % for >15 % (p = 0.11), or on the number of spermatozoa with normal morphology: 50 % for ≥4 % and 45.5 % for <4 % (p = 0,23). In patients after IVF with intracytoplasmic sperm injection, the concentration of spermatozoa, number of progressive-motile sperm (grade А), and number of spermatozoa with normal morphology didn’t affect the frequency of live births in a statistically significant way.Conclusion. Sperm concentration, motility, and morphology can affect the frequency of live births in the IVF program, but statistically significant correlation was observed only for sperm concentration. After IVF with intracytoplasmic sperm injection, only sperm morphology affects the frequency of live births, but not in a statistically significant way. 

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