A Quantitative Model-Based Selection of Web Service Reconfiguration

Author(s):  
Honghao Gao ◽  
Huaikou Miao
2001 ◽  
Vol 7 (S2) ◽  
pp. 578-579
Author(s):  
David W. Knowles ◽  
Sophie A. Lelièvre ◽  
Carlos Ortiz de Solόrzano ◽  
Stephen J. Lockett ◽  
Mina J. Bissell ◽  
...  

The extracellular matrix (ECM) plays a critical role in directing cell behaviour and morphogenesis by regulating gene expression and nuclear organization. Using non-malignant (S1) human mammary epithelial cells (HMECs), it was previously shown that ECM-induced morphogenesis is accompanied by the redistribution of nuclear mitotic apparatus (NuMA) protein from a diffuse pattern in proliferating cells, to a multi-focal pattern as HMECs growth arrested and completed morphogenesis . A process taking 10 to 14 days.To further investigate the link between NuMA distribution and the growth stage of HMECs, we have investigated the distribution of NuMA in non-malignant S1 cells and their malignant, T4, counter-part using a novel model-based image analysis technique. This technique, based on a multi-scale Gaussian blur analysis (Figure 1), quantifies the size of punctate features in an image. Cells were cultured in the presence and absence of a reconstituted basement membrane (rBM) and imaged in 3D using confocal microscopy, for fluorescently labeled monoclonal antibodies to NuMA (fαNuMA) and fluorescently labeled total DNA.


Author(s):  
Jonathan Vaughan ◽  
David A. Rosenbaum ◽  
Cathleen Moore

Author(s):  
M. Tambas ◽  
H.P. van der Laan ◽  
A.V.D. Hoek ◽  
H.P. Bijl ◽  
M. Dieters ◽  
...  

2016 ◽  
Vol 15 (1) ◽  
Author(s):  
John H. Huber ◽  
Geoffrey L. Johnston ◽  
Bryan Greenhouse ◽  
David L. Smith ◽  
T. Alex Perkins

Author(s):  
Guo-qi Liu ◽  
Zhi-liang Zhu ◽  
Yi-qiang Li ◽  
Dan-cheng Li ◽  
Ying Liu

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