Self-Organizing Neural Networks using the Initial Weight Optimization for High-Throughput Screening Systems for the SICE-ICASE International Joint Conference 2006 (SICE-ICCAS 2006)

2006 ◽  
Author(s):  
Sookil Kang ◽  
Sunwon
Keyword(s):  
Neural Networks ◽  
High Throughput ◽  
High Throughput Screening ◽  
Weight Optimization ◽  
Initial Weight ◽  
Joint Conference ◽  
International Joint ◽  
Screening Systems ◽  
Self Organizing

scholarly journals A 96-multiplex capillary electrophoresis screening platform for product based evolution of P450 BM3

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Anna Gärtner ◽  
Anna Joëlle Ruff ◽  
Ulrich Schwaneberg
Keyword(s):  
Capillary Electrophoresis ◽  
High Throughput ◽  
High Throughput Screening ◽  
Product Formation ◽  
Resonance Spectroscopy ◽  
P450 Bm3 ◽  
Microtiter Plates ◽  
Main Challenge ◽  
Total Turnover ◽  
Screening Systems

Abstract The main challenge that prevents a broader application of directed enzyme evolution is the lack of high-throughput screening systems with universal product analytics. Most directed evolution campaigns employ screening systems based on colorimetric or fluorogenic surrogate substrates or universal quantification methods such as nuclear magnetic resonance spectroscopy or mass spectrometry, which have not been advanced to achieve a high-throughput. Capillary electrophoresis with a universal UV-based product detection is a promising analytical tool to quantify product formation. Usage of a multiplex system allows the simultaneous measurement with 96 capillaries. A 96-multiplexed capillary electrophoresis (MP-CE) enables a throughput that is comparable to traditional direct evolution campaigns employing 96-well microtiter plates. Here, we report for the first time the usage of a MP-CE system for directed P450 BM3 evolution towards increased product formation (oxidation of alpha-isophorone to 4-hydroxy-isophorone; highest reached total turnover number after evolution campaign: 7120 mol4-OH molP450−1). The MP-CE platform was 3.5-fold more efficient in identification of beneficial variants than the standard cofactor (NADPH) screening system.

Evolution inspired engineering of antibiotic biosynthesis enzymes

2017 ◽  
Vol 15 (19) ◽  
pp. 4036-4041 ◽  
Author(s):  
M. Metsä-Ketelä
Keyword(s):  
Structure Function ◽  
High Throughput ◽  
High Throughput Screening ◽  
Antibiotic Biosynthesis ◽  
Screening Systems ◽  
Proof Of Principle

Chimeragenesis is an effective tool to probe the structure/function relationships of proteins without high-throughput screening systems. Here the proof-of-principle is presented with three pairs of proteins.

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