Cloning and function identification of the promoters and terminator of sugar beet chloroplast gene psbA

2011 ◽  
Author(s):  
Jie Cui ◽  
Wenjing Li ◽  
Dayou Cheng ◽  
Xin Yang ◽  
Binsheng Li
Keyword(s):  
Sugar Beet ◽  
Chloroplast Gene ◽  
Function Identification ◽  
And Function

The Preparation and Function Identification of a Novel Anti-Fumarate Hydratase Antibody

2013 ◽  
Vol 29 (1) ◽  
pp. 46
Author(s):  
Meihong FU ◽  
Liang LE ◽  
Zhong CHENG ◽  
Yong XIE ◽  
Hai GAO ◽  
...  
Keyword(s):  
Fumarate Hydratase ◽  
Function Identification ◽  
And Function

Definition and function identification of nucleus export signal of SGIV ICP46

2012 ◽  
Vol 36 (11) ◽  
pp. 1763
Author(s):  
Li-qun XIA ◽  
Hong-lian ZHANG ◽  
Qi-wei QIN
Keyword(s):  
Function Identification ◽  
And Function ◽  
Export Signal

scholarly journals Genome-wide identification and expression analysis of the WRKY genes in sugar beet (Beta vulgaris L.) under alkaline stress

2019 ◽  
Author(s):  
Guo-Qiang Wu ◽  
Zhi-Qiang Li ◽  
Han Cao ◽  
Jin-Long Wang
Keyword(s):  
Sugar Beet ◽  
Beta Vulgaris ◽  
Wrky Transcription Factor ◽  
Alkaline Stress ◽  
Expression Levels ◽  
Physiological Processes ◽  
Genome Wide ◽  
Intron Structure ◽  
Software Distribution ◽  
And Function

Background: The WRKY transcription factor family plays critical roles in many aspects of physiological processes and adaption to environment. Although the WRKY genes have been widely studied in various plants, the structure and function of the WRKY family in sugar beet (Beta vulgaris L.) remains unknown. Methods: In the present study, the WRKY genes were identified from the sugar beet genome by bioinformatics. Phylogenetic tree was constructed by MEGA7.0 software. Distribution map of these genes was displayed by MapInspect 1.0. Furthermore, the exon-intron structure and the conserved motifs were predicted by GSDS 2.0 and MEME 5.0.5, respectively. Additionally, the expression levels of these genes under alkaline stress were assayed by qRT-PCR. Results: A total of 58 putative BvWRKY genes are identified in the sugar beet genome. The coding sequence of these genes ranged from 558 to 2,307 bp and molecular weight varied from 21.3 to 84. Based on the conserved WRKY domain and zinc-finger motif, the BvWRKY genes are clustered into three major groups I, II and III, with 11, 40 and 7 genes, respectively. The number of intron in the BvWRKY genes ranged from 1 to 5, with majority of BvWRKY (27/58) containing three exons. All the BvWRKY genes have one or two WRKY motifs at the N-terminus. Moreover, the expression levels of BvWRKY genes are increased remarkedly by alkaline stress. Our findings extend understandings of the BvWRKY genes family and provide useful information for subsequent research on their functions in sugar beet under alkaline stress.

Facilitated Expression and Function Identification of Key Genes Converting Cyanocobalamin to Adenosylcobalamin

2012 ◽  
Vol 18 (2) ◽  
pp. 267
Author(s):  
Weifeng ZHAI ◽  
Huiying FANG ◽  
Bin ZHUGE ◽  
Cheng ZHANG ◽  
Yingmin XUE ◽  
...  
Keyword(s):  
Key Genes ◽  
Function Identification ◽  
And Function

scholarly journals Genome-wide identification and expression analysis of the WRKY genes in sugar beet (Beta vulgaris L.) under alkaline stress

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7817 ◽  
Author(s):  
Guo-Qiang Wu ◽  
Zhi-Qiang Li ◽  
Han Cao ◽  
Jin-Long Wang
Keyword(s):  
Sugar Beet ◽  
Beta Vulgaris ◽  
Expression Patterns ◽  
Wrky Transcription Factor ◽  
Amino Acid Sequences ◽  
Alkaline Stress ◽  
Molecular Weights ◽  
Intron Structure ◽  
Primary Amino ◽  
And Function

Background The WRKY transcription factor family plays crucial roles in many aspects of physiological processes and adaption to environment. Although the WRKY genes have been widely identified in various plant species, the structure and function of the WRKY family in sugar beet (Beta vulgaris L.) remains unknown. Methods In the present study, the WRKY genes were identified from the sugar beet genome by bioinformatics. A phylogenetic tree was constructed by MEGA7.0. A distribution map of these genes was displayed by MapInspect 1.0. Furthermore, the exon-intron structure and the conserved motifs were predicted by GSDS 2.0 and MEME 5.0.5, respectively. Additionally, the expression levels of nine selected genes in shoots and roots of sugar beet seedlings exposed to alkaline stress were assayed by qRT-PCR. Results A total of 58 putative BvWRKY genes are identified in the sugar beet genome. The coding sequences of these genes ranged from 558 to 2,307 bp and molecular weights (MWs) varied from 21.3 to 84. The BvWRKY genes are clustered into three major groups I, II, and III, with 11, 40, and seven members, based on the primary amino acid sequences. The number of introns in the BvWRKY genes ranged from 1 to 5, with a majority of BvWRKY (27/58) containing three exons. All the BvWRKY genes have one or two conserved WRKY domains and zinc-finger structure. Moreover, the selected BvWRKY genes showed a variety of expression patterns in shoots and roots of seedlings under various concentrations of NaHCO3. Importantly, BvWRKY10 in shoots and BvWRKY16 in roots were remarkably up-regulated by alkaline stress. Taken together, our findings extend understandings of the BvWRKY genes family and provide useful information for subsequent research on their functions in sugar beet under alkaline stress.

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