A new stiffness evaluation toward high speed cell sorter

2010 ◽  
Author(s):  
Yuki Hirose ◽  
Kenjiro Tadakuma ◽  
Mitsuru Higashimori ◽  
Tatsuo Arai ◽  
Makoto Kaneko ◽  
...  
Keyword(s):  
High Speed ◽  
Cell Sorter ◽  
Stiffness Evaluation

Stability of the breakoff point in a high-speed cell sorter

Cytometry ◽  
2003 ◽  
Vol 56A (2) ◽  
pp. 63-70 ◽  
Author(s):  
Timothy W. Petersen ◽  
Ger van den Engh
Keyword(s):  
High Speed ◽  
Cell Sorter

3D Pulsed Laser Triggered High Speed Microfluidic Fluorescence Activated Cell Sorter

CLEO: 2013 ◽  
2013 ◽  
Author(s):  
Yue Chen ◽  
Ting-Hsiang Wu ◽  
Yu-Chun Kung ◽  
Michael A. Teitell ◽  
Eric Pei-Yu Chiou
Keyword(s):  
High Speed ◽  
Pulsed Laser ◽  
Cell Sorter

scholarly journals 3D pulsed laser-triggered high-speed microfluidic fluorescence-activated cell sorter

The Analyst ◽  
2013 ◽  
Vol 138 (24) ◽  
pp. 7308 ◽  
Author(s):  
Yue Chen ◽  
Ting-Hsiang Wu ◽  
Yu-Chun Kung ◽  
Michael A. Teitell ◽  
Pei-Yu Chiou
Keyword(s):  
High Speed ◽  
Pulsed Laser ◽  
Cell Sorter

Characterization of Switching Time and Cell Stress in a Gravity-Driven Microfluidic Cell Sorter Based on Hydrodynamic Switching

2010 ◽  
Author(s):  
Michael Grad ◽  
Lubomir Smilenov ◽  
David Brenner ◽  
Daniel Attinger
Keyword(s):  
High Speed ◽  
Bystander Effect ◽  
Fluorescent Protein ◽  
Switching Time ◽  
Blue Dye ◽  
Adhesive Tape ◽  
Buffer Solution ◽  
Cell Sorter ◽  
Microfluidic Cell Sorter

In this work we describe the control and characterization of the switching time and hydrodynamic stress in a microfluidic cell sorter. The device was designed to sort small (<1000) populations of live cells in buffer solution labeled with standard bio-markers such as live dyes or green fluorescent protein (GFP). Sorting occurs through a hydrodynamic switching technique where high-speed solenoid valves control a sheath flow used to steer sorted cells away from the unsorted bulk population. The device is constructed from a reusable hard plastic polymethylmethacrylate (PMMA) chip machined with 127μm × 50μm microchannels and sealed with adhesive tape. Open reservoirs in the chip facilitate pipette access, standard microscope visualization, and a simple disassembly and cleaning procedure. The sorting frequency of this type of device is typically limited by the hydrodynamic switching time. Here, we present a theoretical and numerical analysis of the device switching time. These results show that the sorter switching time t is practically limited by the velocity of the flow and the characteristic length between inlet and outlet channels. We validate this theoretical result with experimental data obtained from flow visualizations, along with experiments conducted to evaluate the repeatability of the hydrodynamic switching scheme and the survival rate of sorted fibroblast cells Manually operated, the sorting frequencies were approximately 10 cells per minute, with switching time constants of approximately 130ms. Current throughput is limited by this switching time to approximately 450 cells per minute. Automation can increase the velocity and reduce the spacing between cells, thereby increasing throughput by at least an order of magnitude. The cell sorter was then tested by manually sorting 100 beads in 7 minutes, and 30 cells in less than 3 minutes, and was successfully used in the framework of a study on the bystander effect occurring during cell irradiation. Experiments with Trypan Blue dye verified that cell viability was maintained during the sorting process.

Optical monitor for measuring the amplitude and phase of perturbations on the surface of a capillary jet in a high-speed cell sorter

2004 ◽  
Vol 75 (3) ◽  
pp. 741-746
Author(s):  
Bob M. Lansdorp ◽  
Peter I. Nelson ◽  
Timothy W. Petersen ◽  
Ger van den Engh
Keyword(s):  
High Speed ◽  
Cell Sorter

scholarly journals High-speed cell sorter

2012 ◽  
Vol 6 (5) ◽  
pp. 269-269 ◽  
Author(s):  
James Baxter
Keyword(s):  
High Speed ◽  
Cell Sorter

High speed cell stiffness evaluation toward 100% reliability

2011 ◽  
Author(s):  
Yuki Hirose ◽  
Makoto Kaneko ◽  
Tomohiro Kawahara ◽  
Yoko Yamanishi ◽  
Fumihito Arai
Keyword(s):  
High Speed ◽  
Cell Stiffness ◽  
Stiffness Evaluation

scholarly journals Online Red Blood Cell Stiffness Evaluation System by Utilizing a High Speed Vision and Micro Channel

2011 ◽  
Vol 47 (5) ◽  
pp. 221-229 ◽  
Author(s):  
Yuki HIROSE ◽  
Wataru FUKUI ◽  
Mitsuru HIGASHIMORI ◽  
Kenjiro TADAKUMA ◽  
Makoto KANEKO ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Evaluation System ◽  
High Speed ◽  
Cell Stiffness ◽  
Micro Channel ◽  
Stiffness Evaluation
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