Stability of the breakoff point in a high-speed cell sorter

Cytometry ◽  
2003 ◽  
Vol 56A (2) ◽  
pp. 63-70 ◽  
Author(s):  
Timothy W. Petersen ◽  
Ger van den Engh
Keyword(s):  
High Speed ◽  
Cell Sorter

3D Pulsed Laser Triggered High Speed Microfluidic Fluorescence Activated Cell Sorter

CLEO: 2013 ◽  
2013 ◽  
Author(s):  
Yue Chen ◽  
Ting-Hsiang Wu ◽  
Yu-Chun Kung ◽  
Michael A. Teitell ◽  
Eric Pei-Yu Chiou
Keyword(s):  
High Speed ◽  
Pulsed Laser ◽  
Cell Sorter

scholarly journals 3D pulsed laser-triggered high-speed microfluidic fluorescence-activated cell sorter

The Analyst ◽  
2013 ◽  
Vol 138 (24) ◽  
pp. 7308 ◽  
Author(s):  
Yue Chen ◽  
Ting-Hsiang Wu ◽  
Yu-Chun Kung ◽  
Michael A. Teitell ◽  
Pei-Yu Chiou
Keyword(s):  
High Speed ◽  
Pulsed Laser ◽  
Cell Sorter

Characterization of Switching Time and Cell Stress in a Gravity-Driven Microfluidic Cell Sorter Based on Hydrodynamic Switching

2010 ◽  
Author(s):  
Michael Grad ◽  
Lubomir Smilenov ◽  
David Brenner ◽  
Daniel Attinger
Keyword(s):  
High Speed ◽  
Bystander Effect ◽  
Fluorescent Protein ◽  
Switching Time ◽  
Blue Dye ◽  
Adhesive Tape ◽  
Buffer Solution ◽  
Cell Sorter ◽  
Microfluidic Cell Sorter

In this work we describe the control and characterization of the switching time and hydrodynamic stress in a microfluidic cell sorter. The device was designed to sort small (<1000) populations of live cells in buffer solution labeled with standard bio-markers such as live dyes or green fluorescent protein (GFP). Sorting occurs through a hydrodynamic switching technique where high-speed solenoid valves control a sheath flow used to steer sorted cells away from the unsorted bulk population. The device is constructed from a reusable hard plastic polymethylmethacrylate (PMMA) chip machined with 127μm × 50μm microchannels and sealed with adhesive tape. Open reservoirs in the chip facilitate pipette access, standard microscope visualization, and a simple disassembly and cleaning procedure. The sorting frequency of this type of device is typically limited by the hydrodynamic switching time. Here, we present a theoretical and numerical analysis of the device switching time. These results show that the sorter switching time t is practically limited by the velocity of the flow and the characteristic length between inlet and outlet channels. We validate this theoretical result with experimental data obtained from flow visualizations, along with experiments conducted to evaluate the repeatability of the hydrodynamic switching scheme and the survival rate of sorted fibroblast cells Manually operated, the sorting frequencies were approximately 10 cells per minute, with switching time constants of approximately 130ms. Current throughput is limited by this switching time to approximately 450 cells per minute. Automation can increase the velocity and reduce the spacing between cells, thereby increasing throughput by at least an order of magnitude. The cell sorter was then tested by manually sorting 100 beads in 7 minutes, and 30 cells in less than 3 minutes, and was successfully used in the framework of a study on the bystander effect occurring during cell irradiation. Experiments with Trypan Blue dye verified that cell viability was maintained during the sorting process.

A new stiffness evaluation toward high speed cell sorter

2010 ◽  
Author(s):  
Yuki Hirose ◽  
Kenjiro Tadakuma ◽  
Mitsuru Higashimori ◽  
Tatsuo Arai ◽  
Makoto Kaneko ◽  
...  
Keyword(s):  
High Speed ◽  
Cell Sorter ◽  
Stiffness Evaluation

Optical monitor for measuring the amplitude and phase of perturbations on the surface of a capillary jet in a high-speed cell sorter

2004 ◽  
Vol 75 (3) ◽  
pp. 741-746
Author(s):  
Bob M. Lansdorp ◽  
Peter I. Nelson ◽  
Timothy W. Petersen ◽  
Ger van den Engh
Keyword(s):  
High Speed ◽  
Cell Sorter

scholarly journals High-speed cell sorter

2012 ◽  
Vol 6 (5) ◽  
pp. 269-269 ◽  
Author(s):  
James Baxter
Keyword(s):  
High Speed ◽  
Cell Sorter

scholarly journals Pulsed laser triggered high speed microfluidic fluorescence activated cell sorter

Lab on a Chip ◽  
2012 ◽  
Vol 12 (7) ◽  
pp. 1378 ◽  
Author(s):  
Ting-Hsiang Wu ◽  
Yue Chen ◽  
Sung-Yong Park ◽  
Jason Hong ◽  
Tara Teslaa ◽  
...  
Keyword(s):  
High Speed ◽  
Pulsed Laser ◽  
Cell Sorter

Abstract 566: Angiotensin II Promotes Proliferation and Fibrosis in Parietal Epithelial Cells Contributing to the Development of Crescentic Glomerulonephritis

Hypertension ◽  
2013 ◽  
Vol 62 (suppl_1) ◽  
Author(s):  
Yuki Shibayama ◽  
Maki Urushihara ◽  
Akira Nishiyama ◽  
Hiroyuki Kobori
Keyword(s):  
Cell Proliferation ◽  
Epithelial Cells ◽  
High Speed ◽  
Crescentic Glomerulonephritis ◽  
Ang Ii ◽  
Cell Sorter ◽  
Soluble Collagen ◽  
Collagen Secretion ◽  
Proliferation Ability ◽  
Parietal Epithelial Cells

Introduction: Parietal epithelial cells (PECs) contribute to the glomerular crescent formation of crescentic glomerulonephritis. Co-cultured model consisting of mesangial cells (MCs), PECs, and macrophages which play a pivotal role in the development of crescentic glomerulonephritis showed an increase in Angiotensin (Ang) II-induced cell proliferation and collagen secretion in PECs. Moreover, Ang II type 1 receptor blocker (ARB) treatment completely prevented an increase in cell proliferation and collagen secretion in PECs under the co-cultured model. However, it is not clear whether Ang II directly promotes PEC fibrosis and proliferation under the monoculture in PECs. Aim: The aim of present study is to demonstrate that Ang II stimulation directly affects PEC proliferation and fibrosis. Methods: Primary fluorescein isothiocyanate (FITC)-labeled PEC population was collected from glomeruli in rats using high-speed cell sorter. ERK1/2 phosphorylation was evaluated by Western blotting. Cell proliferation ability was measured by water-soluble tetrazolium salts (WST)-1 and the soluble collagen levels in culture supernatants was determined by the Sircol collagen assay. Results: FITC-labeled PECs could be identified as a distinct cell population by high-speed cell sorter. The ERK 1/2 phosphorylation in PECs was stimulated by Ang II (100 nmol/l) by about 1.7-fold. After the treatment with 100 nmol/l Ang II for 24 hrs, cell proliferation ability significantly increased in PECs (mean ± SEM: 1.60 ± 0.03 vs. 1.00 ± 0.04, relative ratio). However, the proliferation ability in Ang II-stimulated PECs was suppressed by Ang II plus 10 nmol/l olmesartan (an ARB) or Ang II plus 100 nmol/l PD98059 (a MEK inhibitor). PECs also promoted a collagen secretion after the stimulation by 100 nmol/l Ang II for 24 hrs (52.1 ± 8.1 vs. 16.8 ± 2.4 μg/ml). Furthermore, the significant decrease in soluble collagen secretion was observed by the treatments with Ang II plus 10 nmol/l olmesartan or Ang II plus 10 μg/ml pan-specific neutralizing TGF-β antibody. Conclusion: These data indicate that Ang II-stimulated PECs promote proliferation and fibrosis and may suggest contributing to the development of crescentic glomerulonephritis by PECs independently from MCs or macrophages.

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