Visual-based Impedance Force Control of Three-dimensional Cell Injection System

2007 ◽  
Author(s):  
Haibo Huang ◽  
Dong Sun ◽  
James K. Mills ◽  
Wen J. Li
Keyword(s):  
Force Control ◽  
Three Dimensional ◽  
Injection System ◽  
Cell Injection ◽  
Dimensional Cell

Fundamental research of a 3-dimensional cell injection system

2004 ◽  
Vol 2004 (0) ◽  
pp. 207-208
Author(s):  
Yanbin SUN ◽  
Toru MASUZAKI ◽  
Takayuki SHIBATA ◽  
Akio KISHIDA
Keyword(s):  
Injection System ◽  
Cell Injection ◽  
3 Dimensional ◽  
Fundamental Research ◽  
Dimensional Cell

Robotic Cell Injection System With Position and Force Control: Toward Automatic Batch Biomanipulation

2009 ◽  
Vol 25 (3) ◽  
pp. 727-737 ◽  
Author(s):  
H.B. Huang ◽  
Dong Sun ◽  
J.K. Mills ◽  
Shuk Han Cheng
Keyword(s):  
Force Control ◽  
Injection System ◽  
Robotic Cell ◽  
Cell Injection ◽  
Automatic Batch

scholarly journals Can keratin scaffolds be used for creating three-dimensional cell cultures?

Open Medicine ◽  
2020 ◽  
Vol 15 (1) ◽  
pp. 249-253
Author(s):  
Marta Bochynska-Czyz ◽  
Patrycja Redkiewicz ◽  
Hanna Kozlowska ◽  
Joanna Matalinska ◽  
Marek Konop ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Chemical Activation ◽  
Three Dimensional ◽  
Enzymatic Digestion ◽  
Pepsin Digestion ◽  
Cell Culturing ◽  
3D Cell Cultures ◽  
Associated Proteins ◽  
Positive Effect ◽  
Dimensional Cell

AbstractThree-dimensional (3D) cell cultures were created with the use of fur keratin associated proteins (F-KAPs) as scaffolds. The procedure of preparation F-KAP involves combinations of chemical activation and enzymatic digestion. The best result in porosity and heterogeneity of F-KAP surface was received during pepsin digestion. The F-KAP had a stable structure, no changes were observed after heat treatment, shaking and washing. The 0.15-0.5 mm fraction had positive effect for formation of 3D scaffolds and cell culturing. Living rat mesenchymal cells on the F-KAP with no abnormal morphology were observed by SEM during 32 days of cell culturing.

scholarly journals Factors to consider when interrogating 3D culture models with plate readers or automated microscopes

2021 ◽  
Author(s):  
Terry Riss ◽  
O. Joseph Trask
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
3D Culture ◽  
Fluorescent Imaging ◽  
Culture Models ◽  
Assay Methods ◽  
Diffusion Rates ◽  
Cell Culture Models ◽  
Dimensional Cell ◽  
Cells Cultured

AbstractAlong with the increased use of more physiologically relevant three-dimensional cell culture models comes the responsibility of researchers to validate new assay methods that measure events in structures that are physically larger and more complex compared to monolayers of cells. It should not be assumed that assays designed using monolayers of cells will work for cells cultured as larger three-dimensional masses. The size and barriers for penetration of molecules through the layers of cells result in a different microenvironment for the cells in the outer layer compared to the center of three-dimensional structures. Diffusion rates for nutrients and oxygen may limit metabolic activity which is often measured as a marker for cell viability. For assays that lyse cells, the penetration of reagents to achieve uniform cell lysis must be considered. For live cell fluorescent imaging assays, the diffusion of fluorescent probes and penetration of photons of light for probe excitation and fluorescent emission must be considered. This review will provide an overview of factors to consider when implementing assays to interrogate three dimensional cell culture models.

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