Single molecule and cell manipulation in soft microfluidic devices

2002 ◽  
Author(s):  
S. Quake
Keyword(s):  
Single Molecule ◽  
Microfluidic Devices ◽  
Cell Manipulation

Single molecule detection of double-stranded DNA in poly(methylmethacrylate) and polycarbonate microfluidic devices

2001 ◽  
Vol 22 (18) ◽  
pp. 3939-3948 ◽  
Author(s):  
Musundi B. Wabuyele ◽  
Sean M. Ford ◽  
Wieslaw Stryjewski ◽  
James Barrow ◽  
Steven A. Soper
Keyword(s):  
Single Molecule ◽  
Microfluidic Devices ◽  
Single Molecule Detection ◽  
Double Stranded Dna

Single-cell manipulation and analysis using microfluidic devices

2006 ◽  
Vol 387 (1) ◽  
pp. 9-12 ◽  
Author(s):  
Gregory T. Roman ◽  
Yanli Chen ◽  
Pernilla Viberg ◽  
Anne H. Culbertson ◽  
Christopher T. Culbertson
Keyword(s):  
Single Cell ◽  
Microfluidic Devices ◽  
Cell Manipulation ◽  
Single Cell Manipulation

SIZE-CONTROLLED DROPLET GENERATION IN A MICROFLUIDIC DEVICE FOR RARE DNA AMPLIFICATION BY OPTIMIZING ITS EFFECTIVE PARAMETERS

2018 ◽  
Vol 18 (01) ◽  
pp. 1850002 ◽  
Author(s):  
ALI LASHKARIPOUR ◽  
ALI ABOUEI MEHRIZI ◽  
MASOUD GOHARIMANESH ◽  
MOHAMMADREZA RASOULI ◽  
SAJAD RAZAVI BAZAZ
Keyword(s):  
Single Molecule ◽  
Dominant Role ◽  
Dna Amplification ◽  
Microfluidic Devices ◽  
Sample Volume ◽  
Droplet Generation ◽  
Droplet Radius ◽  
Generation Process ◽  
Effective Parameters ◽  
Precise Control

Versatility and portability of microfluidic devices play a dominant role in their widespread use by researchers. Droplet-based microfluidic devices have been extensively used due to their precise control over sample volume, and ease of manipulating and addressing each droplet on demand. Droplet-based polymerase chain reaction (PCR) devices are particularly desirable in single DNA amplification. If the droplets are small enough to contain only one DNA molecule, single molecule amplification becomes possible, which can be advantageous in several cases such as early cancer detection. In this work, flow-focusing microfluidic droplet generation’s parameters are numerically investigated and optimized for generating the smallest droplet possible, while considering fabrication limits. Taguchi design of experiment method is used to study the effects of key parameters in droplet generation. By exploiting this approach, a droplet with a radius of 111[Formula: see text]nm is generated using a 3[Formula: see text][Formula: see text]m orifice. Since the governing physics of the droplet generation process is not totally understood yet, by means of analysis of variance (ANOVA) analysis, a generalized linear model (GLM) is proposed to predict the droplet radius, given the values of eight major parameters affecting the droplet size. The proposed model shows a correlation of 95.3% and 64.95% for droplets of radius greater than and lower than 5[Formula: see text][Formula: see text]m, respectively. Finally, the source of this variation of behavior in different size scales is identified.

Investigation of Automated Cell Manipulation in Optical Tweezers-Assisted Microfluidic Chamber Using Simulations

2011 ◽  
Author(s):  
Sagar Chowdhury ◽  
Petr Svec ◽  
Chenlu Wang ◽  
Kevin T. Seale ◽  
John P. Wikswo ◽  
...  
Keyword(s):  
Fluid Flow ◽  
Optical Tweezers ◽  
High Precision ◽  
High Throughput ◽  
Microfluidic Devices ◽  
Cell Manipulation ◽  
Precise Control ◽  
Biological Objects ◽  
Microfluidic Chamber ◽  
Manipulation Process

Microfluidic devices are well suited for the study of biological objects because of their indirect nature of manipulation and high throughput. However, the cell manipulation process solely depends on the fluid flow and hence precise control is difficult to attain inside a microfluidic chamber. Utilizing optical tweezers as a complementary tool provides precise manipulation control. We have presented an automated cell manipulation approach using optical tweezers operating inside a microfluidic chamber. To test and demonstrate the effectiveness of the approach we have developed a physics-based simulator that is completely automated and allows high precision of manipulation.

scholarly journals Single-Molecule Imaging of NGF Axonal Transport in Microfluidic Devices

2011 ◽  
Vol 100 (3) ◽  
pp. 256a
Author(s):  
Kai Zhang ◽  
Yasuko Osakada ◽  
Marija Vrljic ◽  
Liang Chen ◽  
Harsha V. Mudrakola ◽  
...  
Keyword(s):  
Axonal Transport ◽  
Single Molecule ◽  
Microfluidic Devices ◽  
Single Molecule Imaging

Tunable Confinement for Bridging Single-Cell Manipulation and Single-Molecule DNA Linearization

Small ◽  
2018 ◽  
Vol 14 (17) ◽  
pp. 1800229 ◽  
Author(s):  
Miao Yu ◽  
Youmin Hou ◽  
Ruyuan Song ◽  
Xiaonan Xu ◽  
Shuhuai Yao
Keyword(s):  
Single Cell ◽  
Single Molecule ◽  
Cell Manipulation ◽  
Single Cell Manipulation

scholarly journals Single cell manipulation, analytics, and label-free protein detection in microfluidic devices for systems nanobiology

2005 ◽  
Vol 26 (19) ◽  
pp. 3689-3696 ◽  
Author(s):  
Wibke Hellmich ◽  
Christoph Pelargus ◽  
Kai Leffhalm ◽  
Alexandra Ros ◽  
Dario Anselmetti
Keyword(s):  
Single Cell ◽  
Protein Detection ◽  
Microfluidic Devices ◽  
Cell Manipulation ◽  
Label Free ◽  
Free Protein ◽  
Single Cell Manipulation
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