Development of in‐flow label‐free single molecule sensors using planar solid‐state nanopore integrated microfluidic devices

2018 ◽  
Vol 13 (9) ◽  
pp. 1352-1357 ◽  
Author(s):  
Fatma D. Güzel ◽  
Benjamin Miles
Keyword(s):  
Solid State ◽  
Single Molecule ◽  
Microfluidic Devices ◽  
Label Free ◽  
Flow Label

scholarly journals Label-free single-molecule identification of telomere G-quadruplexes with a solid-state nanopore sensor

RSC Advances ◽  
2020 ◽  
Vol 10 (45) ◽  
pp. 27215-27224 ◽  
Author(s):  
Sen Wang ◽  
Liyuan Liang ◽  
Jing Tang ◽  
Yao Cai ◽  
Chuanqi Zhao ◽  
...  
Keyword(s):  
Solid State ◽  
Single Molecule ◽  
Label Free

Nanopore detection of single-molecule G-quadruplexes.

scholarly journals A Solid-State Nanopore-Based Single-Molecule Approach for Label-free Characterization of Plant Polysaccharides

2020 ◽  
pp. 100106
Author(s):  
Yao Cai ◽  
Baocai Zhang ◽  
Liyuan Liang ◽  
Sen Wang ◽  
Lanjun Zhang ◽  
...  
Keyword(s):  
Solid State ◽  
Single Molecule ◽  
Label Free ◽  
Plant Polysaccharides

Label-free, mass-sensitive single-molecule imaging using interferometric scattering microscopy

2020 ◽  
Author(s):  
Nikolas Hundt
Keyword(s):  
Single Molecule ◽  
Cellular Systems ◽  
Single Molecule Imaging ◽  
Label Free ◽  
Measurement Sensitivity ◽  
Mass Distributions ◽  
Quantitative Measurements ◽  
Contrast Mechanism ◽  
Cellular Components ◽  
Scattering Signal

Abstract Single-molecule imaging has mostly been restricted to the use of fluorescence labelling as a contrast mechanism due to its superior ability to visualise molecules of interest on top of an overwhelming background of other molecules. Recently, interferometric scattering (iSCAT) microscopy has demonstrated the detection and imaging of single biomolecules based on light scattering without the need for fluorescent labels. Significant improvements in measurement sensitivity combined with a dependence of scattering signal on object size have led to the development of mass photometry, a technique that measures the mass of individual molecules and thereby determines mass distributions of biomolecule samples in solution. The experimental simplicity of mass photometry makes it a powerful tool to analyse biomolecular equilibria quantitatively with low sample consumption within minutes. When used for label-free imaging of reconstituted or cellular systems, the strict size-dependence of the iSCAT signal enables quantitative measurements of processes at size scales reaching from single-molecule observations during complex assembly up to mesoscopic dynamics of cellular components and extracellular protrusions. In this review, I would like to introduce the principles of this emerging imaging technology and discuss examples that show how mass-sensitive iSCAT can be used as a strong complement to other routine techniques in biochemistry.

Single Molecule Solid State Light Emitting Electrochemical Cells with Lifetimes Superior to 3000 Hours

2008 ◽  
Author(s):  
Henk Bolink ◽  
Rubén D. Costa ◽  
Enrique Orti ◽  
Michele Sessolo ◽  
Stefan Graber ◽  
...  
Keyword(s):  
Solid State ◽  
Single Molecule ◽  
Electrochemical Cells ◽  
Light Emitting

scholarly journals Solid‐State Near‐Infrared Circularly Polarized Luminescence from Chiral YbIII‐Single‐Molecule Magnet

2021 ◽  
Author(s):  
Fabrice Pointillart ◽  
Bertrand Lefeuvre ◽  
Carlo Andrea Mattei ◽  
Jessica Flores Gonzalez ◽  
Frédéric Gendron ◽  
...  
Keyword(s):  
Solid State ◽  
Single Molecule ◽  
Near Infrared ◽  
Circularly Polarized ◽  
Single Molecule Magnet ◽  
Circularly Polarized Luminescence ◽  
Polarized Luminescence

scholarly journals Biological Nanopores: Engineering on Demand

Life ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 27
Author(s):  
Ana Crnković ◽  
Marija Srnko ◽  
Gregor Anderluh
Keyword(s):  
Molecular Biology ◽  
Single Molecule ◽  
Enzymatic Reactions ◽  
Label Free ◽  
Natural Sources ◽  
On Demand ◽  
Label Free Detection ◽  
Long Read ◽  
Inorganic Molecules ◽  
Standard Molecular Biology

Nanopore-based sensing is a powerful technique for the detection of diverse organic and inorganic molecules, long-read sequencing of nucleic acids, and single-molecule analyses of enzymatic reactions. Selected from natural sources, protein-based nanopores enable rapid, label-free detection of analytes. Furthermore, these proteins are easy to produce, form pores with defined sizes, and can be easily manipulated with standard molecular biology techniques. The range of possible analytes can be extended by using externally added adapter molecules. Here, we provide an overview of current nanopore applications with a focus on engineering strategies and solutions.

scholarly journals Label-free single-molecule all-optical sensor

2008 ◽  
Author(s):  
Andrea M. Armani ◽  
Scott E. Fraser ◽  
Richard C. Flagan
Keyword(s):  
Single Molecule ◽  
Optical Sensor ◽  
Label Free ◽  
All Optical

Measuring Trapped DNA at the Liquid-Air Interface for Enhanced Single Molecule Sensing

Nanoscale ◽  
2021 ◽  
Author(s):  
Nasim Farajpour ◽  
Lauren Lastra ◽  
Vinay Sharma ◽  
Kevin Freedman
Keyword(s):  
Solid State ◽  
Single Molecule ◽  
Borosilicate Glass ◽  
Low Noise ◽  
Viable Alternative ◽  
Single Molecule Detection ◽  
Widespread Application ◽  
Promising Tool ◽  
Air Interface ◽  
Cost Efficient

Nanopore sensing is a promising tool with widespread application in single-molecule detection. Borosilicate glass nanopores are a viable alternative to other solid-state nanopores due to low noise and cost-efficient fabrication....

Single-molecule nucleic acid interactions monitored on a label-free microcavity biosensor platform

2014 ◽  
Vol 9 (11) ◽  
pp. 933-939 ◽  
Author(s):  
Martin D. Baaske ◽  
Matthew R. Foreman ◽  
Frank Vollmer
Keyword(s):  
Nucleic Acid ◽  
Single Molecule ◽  
Label Free ◽  
Nucleic Acid Interactions

scholarly journals Label-free DNA biosensing by topological light confinement

Nanophotonics ◽  
2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Gianluigi Zito ◽  
Gennaro Sanità ◽  
Bryan Guilcapi Alulema ◽  
Sofía N. Lara Yépez ◽  
Vittorino Lanzio ◽  
...  
Keyword(s):  
Single Molecule ◽  
Bound States ◽  
Production Control ◽  
Point Of Care ◽  
Low Cost ◽  
Label Free ◽  
Large Area ◽  
Light Confinement ◽  
Binding Event ◽  
The Continuum

Abstract Large-area and transparent all-dielectric metasurfaces sustaining photonic bound states in the continuum (BICs) provide a set of fundamental advantages for ultrasensitive biosensing. BICs bridge the gap of large effective mode volume with large experimental quality factor. Relying on the transduction mechanism of reactive sensing principle, herein, we first numerically study the potential of subwavelength confinement driven by topological decoupling from free space radiation for BIC-based biosensing. Then, we experimentally combine this capability with minimal and low-cost optical setup, applying the devised quasi-BIC resonator for PNA/DNA selective biosensing with real-time monitoring of the binding event. A sensitivity of 20 molecules per micron squared is achieved, i.e. ≃0.01 pg. Further enhancement can easily be envisaged, pointing out the possibility of single-molecule regime. This work aims at a precise and ultrasensitive approach for developing low-cost point-of-care tools suitable for routine disease prescreening analyses in laboratory, also adaptable to industrial production control.

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