A high-throughput perfusion-based micro three-dimensional cell culture platform

2011 ◽  
Author(s):  
Song-Bin Huang ◽  
Min-Hsien Wu ◽  
Shih-Siou Wang ◽  
Chun Che Lin ◽  
Gwo-Bin Lee
Keyword(s):  
Cell Culture ◽  
High Throughput ◽  
Three Dimensional ◽  
Dimensional Cell

scholarly journals Beta-hairpin hydrogels as scaffolds for high-throughput drug discovery in three-dimensional cell culture

2017 ◽  
Vol 535 ◽  
pp. 25-34 ◽  
Author(s):  
Peter Worthington ◽  
Katherine M. Drake ◽  
Zhiqin Li ◽  
Andrew D. Napper ◽  
Darrin J. Pochan ◽  
...  
Keyword(s):  
Cell Culture ◽  
Drug Discovery ◽  
High Throughput ◽  
Three Dimensional ◽  
Beta Hairpin ◽  
Dimensional Cell

High-throughput generation of spheroids using magnetic nanoparticles for three-dimensional cell culture

Biomaterials ◽  
2013 ◽  
Vol 34 (34) ◽  
pp. 8555-8563 ◽  
Author(s):  
Jeong Ah Kim ◽  
Jong-Ho Choi ◽  
Minsoo Kim ◽  
Won Jong Rhee ◽  
Boram Son ◽  
...  
Keyword(s):  
Cell Culture ◽  
Magnetic Nanoparticles ◽  
High Throughput ◽  
Three Dimensional ◽  
Dimensional Cell

scholarly journals Three-dimensional cell culture microarray for high-throughput studies of stem cell fate

2010 ◽  
pp. n/a-n/a ◽  
Author(s):  
Tiago G. Fernandes ◽  
Seok-Joon Kwon ◽  
Shyam Sundhar Bale ◽  
Moo-Yeal Lee ◽  
Maria Margarida Diogo ◽  
...  
Keyword(s):  
Cell Culture ◽  
Stem Cell ◽  
Cell Fate ◽  
High Throughput ◽  
Three Dimensional ◽  
Stem Cell Fate ◽  
Dimensional Cell

A high throughput perfusion-based microbioreactor platform integrated with pneumatic micropumps for three-dimensional cell culture

2007 ◽  
Vol 10 (2) ◽  
pp. 309-319 ◽  
Author(s):  
Min-Hsien Wu ◽  
Song-Bin Huang ◽  
Zhanfeng Cui ◽  
Zheng Cui ◽  
Gwo-Bin Lee
Keyword(s):  
Cell Culture ◽  
High Throughput ◽  
Three Dimensional ◽  
Dimensional Cell

scholarly journals Factors to consider when interrogating 3D culture models with plate readers or automated microscopes

2021 ◽  
Author(s):  
Terry Riss ◽  
O. Joseph Trask
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
3D Culture ◽  
Fluorescent Imaging ◽  
Culture Models ◽  
Assay Methods ◽  
Diffusion Rates ◽  
Cell Culture Models ◽  
Dimensional Cell ◽  
Cells Cultured

AbstractAlong with the increased use of more physiologically relevant three-dimensional cell culture models comes the responsibility of researchers to validate new assay methods that measure events in structures that are physically larger and more complex compared to monolayers of cells. It should not be assumed that assays designed using monolayers of cells will work for cells cultured as larger three-dimensional masses. The size and barriers for penetration of molecules through the layers of cells result in a different microenvironment for the cells in the outer layer compared to the center of three-dimensional structures. Diffusion rates for nutrients and oxygen may limit metabolic activity which is often measured as a marker for cell viability. For assays that lyse cells, the penetration of reagents to achieve uniform cell lysis must be considered. For live cell fluorescent imaging assays, the diffusion of fluorescent probes and penetration of photons of light for probe excitation and fluorescent emission must be considered. This review will provide an overview of factors to consider when implementing assays to interrogate three dimensional cell culture models.

A novel fluffy hydroxylapatite fiber scaffold with deep interconnected pores designed for three-dimensional cell culture

2014 ◽  
Vol 2 (1) ◽  
pp. 129-136 ◽  
Author(s):  
Lin Jin ◽  
Zhang-Qi Feng ◽  
Ting Wang ◽  
Zhuozhuo Ren ◽  
Shuangshuang Ma ◽  
...  
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
Interconnected Pores ◽  
Dimensional Cell
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