scholarly journals Three-dimensional cell culture microarray for high-throughput studies of stem cell fate

2010 ◽  
pp. n/a-n/a ◽  
Author(s):  
Tiago G. Fernandes ◽  
Seok-Joon Kwon ◽  
Shyam Sundhar Bale ◽  
Moo-Yeal Lee ◽  
Maria Margarida Diogo ◽  
...  
Keyword(s):  
Cell Culture ◽  
Stem Cell ◽  
Cell Fate ◽  
High Throughput ◽  
Three Dimensional ◽  
Stem Cell Fate ◽  
Dimensional Cell

The effects of poly(dimethylsiloxane) surface silanization on the mesenchymal stem cell fate

2015 ◽  
Vol 3 (2) ◽  
pp. 383-390 ◽  
Author(s):  
Yon Jin Chuah ◽  
Shreyas Kuddannaya ◽  
Min Hui Adeline Lee ◽  
Yilei Zhang ◽  
Yuejun Kang
Keyword(s):  
Cell Culture ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Fate ◽  
Stem Cell Fate

Surface silanization with 3-aminopropyl triethoxy silane (APTES) ± glutaraldehyde (GA) enhanced the biocompatibility of poly(dimethylsiloxane) surfaces for long term cell culture investigation.

scholarly journals Beta-hairpin hydrogels as scaffolds for high-throughput drug discovery in three-dimensional cell culture

2017 ◽  
Vol 535 ◽  
pp. 25-34 ◽  
Author(s):  
Peter Worthington ◽  
Katherine M. Drake ◽  
Zhiqin Li ◽  
Andrew D. Napper ◽  
Darrin J. Pochan ◽  
...  
Keyword(s):  
Cell Culture ◽  
Drug Discovery ◽  
High Throughput ◽  
Three Dimensional ◽  
Beta Hairpin ◽  
Dimensional Cell

scholarly journals Growing, Tracking, and Directing Bone Marrow Derived Stem Cells From Two-Dimensional and Three-Dimensional Cell Culture Microenvironments

2019 ◽  
Author(s):  
Tiffany Miller
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Bone Marrow ◽  
Stem Cell ◽  
Three Dimensional ◽  
Inflammatory Biomarkers ◽  
Two Dimensional ◽  
Cell Culturing ◽  
Dimensional Cell ◽  
Stimulation Of

<p>Bone marrow derived stem cells express biomarkers capable of facilitating adhesion to the cell culturing microenvironment, thereby, influencing their proliferation, migration, and differentiation. In particular, biological biomarkers of mesenchymal stem cells include, but are not limited to, CD14-, CD19-, CD34-, CD45-, CD29, CD44, CD73+, CD90+, CD105+, CD106, CD166, Stro-1, and HLADR. The relationship between the stem cell biology and the materials and methods forming a cell culturing microenvironment serves as a critical aspect in the successful adhesion and growth within two-dimensional cell culture microenvironments such as polystyrene, laminin, fibronectin, or poly-L-lysine and within three-dimensional cell culture microenvironments such as hydrogel, ceramic, collagen, polymer based nanofibers, agitation, forced floating, and hang drop systems. Further, electrical stimulation of the stem cells may be implemented during the cell culturing process to measure stem cell growth and to determine stem cell viability. In addition, electrical stimulation of implanted stem cells may facilitate tracking by measuring stem cell migration distance and travel area. Although many biochemical and inflammatory biomarkers are expressed based on severity in stroke including, but not limited to, Interluken-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and glutamate (Glu), current methodologies of stem cell directing lack localization and biological effector specificity. Biological effector bound magnetic particle stem cells may serve as a potential treatment method in ischemic stroke. In particular, a stem cell biomarker may be configured to communicate with inflammatory biomarkers, thus, more efficiently delivering the stem cells to site specific areas having the most severely affected <i>in-vivo</i> biochemical microenvironments.</p>

Artificial niche microarrays for probing single stem cell fate in high throughput

2011 ◽  
Vol 8 (11) ◽  
pp. 949-955 ◽  
Author(s):  
Samy Gobaa ◽  
Sylke Hoehnel ◽  
Marta Roccio ◽  
Andrea Negro ◽  
Stefan Kobel ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Fate ◽  
High Throughput ◽  
Stem Cell Fate

scholarly journals Degradation-mediated cellular traction directs stem cell fate in covalently crosslinked three-dimensional hydrogels

2013 ◽  
Vol 12 (5) ◽  
pp. 458-465 ◽  
Author(s):  
Sudhir Khetan ◽  
Murat Guvendiren ◽  
Wesley R. Legant ◽  
Daniel M. Cohen ◽  
Christopher S. Chen ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Fate ◽  
Three Dimensional ◽  
Stem Cell Fate
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