In-situ adhesion studies of a single living bovine endothelial cell using MEMS sensor

2002 ◽  
Author(s):  
C. Sager ◽  
P. LeDuc ◽  
T. Saif
Keyword(s):  
Endothelial Cell ◽  
Mems Sensor ◽  
Bovine Endothelial Cell ◽  
Single Living

Adhesion Studies of Single Living Cells Using MEMS Sensors

2000 ◽  
Author(s):  
Chad Sager ◽  
Taher Saif ◽  
Phil LeDuc
Keyword(s):  
Endothelial Cell ◽  
Living Cell ◽  
Mems Sensors ◽  
Adhesion Properties ◽  
Mems Sensor ◽  
Bovine Endothelial Cell ◽  
Single Living Cell ◽  
Single Living Cells ◽  
Single Living

Abstract Adhesion between cells is related to several physiological phenomena such as heart failure (Karila00), how cancer spreads (Ruoslahti99) and if an infection will be fought off. Controlling of these events requires knowledge of how cells adhere. Many previous studies have been conducted with various amounts of success. But none of these methods are independently capable of understanding the adhesion properties of a single living cell. In this article a MEMS sensor has been employed to study, quantitatively and qualitatively, the adhesion properties of a single living bovine endothelial cell. This experiment shows that the strength of a single anchorage site of the endothelial cell to an extracellular matrix coated substrate is 36 nN. Anchorage sites have been observed, in-situ, to be spaced on the order of 1 μm intervals. A model is also proposed for the detachment of a single living cell from a substrate.

Cellular mechanism of thrombin on endothelin-1 biosynthesis and release in bovine endothelial cell

1992 ◽  
Vol 44 (12) ◽  
pp. 2409-2411 ◽  
Author(s):  
Toshiaki Emori ◽  
Yukio Hirata ◽  
Taihei Imai ◽  
Kazuki Ohta ◽  
Kazuo Kanno ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cellular Mechanism ◽  
Endothelin 1 ◽  
Bovine Endothelial Cell

scholarly journals Selective internalization of arachidonic acid by endothelial cells

1987 ◽  
Vol 245 (1) ◽  
pp. 151-157 ◽  
Author(s):  
E R Hall ◽  
C E Manner ◽  
J Carinhas ◽  
R Snopko ◽  
M Rafelson
Keyword(s):  
Fatty Acids ◽  
Endothelial Cells ◽  
Arachidonic Acid ◽  
Endothelial Cell ◽  
Polyunsaturated Fatty Acids ◽  
Cell Types ◽  
Time Dependent ◽  
Asymmetric Distribution ◽  
Membrane Phospholipids ◽  
Bovine Endothelial Cell

The asymmetric distribution of phospholipids in bovine endothelial-cell membranes was probed with 2,4,6-trinitrobenzenesulphonate and purified phospholipase A2. The data suggest that phosphotidylethanolamine is primarily located in the inner lipid bilayer, as reported for other cell types. Stearic acid is taken up by the endothelial cells and is randomly distributed among the membrane phospholipids. In contrast, the polyunsaturated fatty acids (arachidonic, eicosatrienoic and eicosapentaenoic acids) have initial incorporation into the phosphatidylcholine fraction. These fatty acids then undergo a time-dependent transfer from phosphatidylcholine to phosphatidylethanolamine. Thus we propose that endothelial cells possess a mechanism for the selective internalization of polyunsaturated fatty acids.

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