Monitoring the glycerol concentration in aqueous glycerol solutions using a micromachined flow sensor

2014 ◽  
Author(s):  
S. Cerimovic ◽  
F. Keplinger ◽  
R. Beigelbeck ◽  
A. Jachimowicz ◽  
H. Antlinger ◽  
...  
Keyword(s):  
Flow Sensor ◽  
Glycerol Concentration

Composition of White Potato Starch (Ipomea batatas L.) with Avocado Seed Starch (Persea americana Mill) and Glyserol Concentration in Edible Film

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Musdar Musdar ◽  
Lukmanul Hakim ◽  
Juliani Juliani ◽  
Jailani Jailani
Keyword(s):  
Sweet Potato ◽  
Potato Starch ◽  
Persea Americana ◽  
Edible Film ◽  
Glycerol Concentration ◽  
White Potato ◽  
Ipomea Batatas ◽  
Sweet Potato Starch ◽  
Local Plants ◽  
Avocado Seed

White sweet potato starch (Ipomea batatas L.) and avocado seed starch (Parsea americana Mill) derived from local plants have the potential to be developed as agricultural products. Starch is a hydrocolloid compound as a potential local resource to be utilized. Glycerol function as an anti-freezing which is hygroscopic. This study aims to determine the ratio of white sweet potato starch with avocado seed starch and the concentration of glycerol for making edible film. This study was an experiment using a completely randimized factorial design with 2 (two) main factor consisting of a comparison of white sweet potato starch and avocado seed with 3 levels: P1 = 35%:65%., P2=50%:50%., P3=65%:35% and glycerol concentration with 3 levels: G1=1%., G2=2%., G3=3%. The best result reasearch were content of 23.03% (tratment P1G1), solubility of 55.57% (treatment P3G2)., swelling test of 9.83% (treatment P2g3)., elongation of 8.18% (treatment P3G2)

PENGARUH PELARUT KLOROFORM DALAM PEMURNIAN GLISEROL DENGAN PROSES ASIDIFIKASI ASAM KLORIDA

2016 ◽  
Vol 5 (3) ◽  
pp. 38-43
Author(s):  
Windi Monica Surbakti ◽  
Gerson Rico M.H ◽  
Mersi Suriani Sinaga
Keyword(s):  
Fatty Acid ◽  
Volume Ratio ◽  
Biodiesel Production ◽  
Extraction Time ◽  
Glycerol Concentration ◽  
Purification Process ◽  
Soap Content ◽  
Test Volume ◽  
Base Content ◽  
Water Salt

Glycerol as a byproduct of biodiesel production was approximately formed 10% of the biodiesel weight. Impurities which contained in the glycerol such as catalyst, soap, methanol, water, salt, and matter organic non glycerol (MONG) have a significant effect on the glycerol concentration. So, it is necessary to treat the impurities. The purpose of this study is to know the effect of chloroform to glycerol purification process with acidification method using hydrochloric acid as pretreatment process. This research was begun with acid addition to the glycerol to neutralize the base content and to split the soap content into free fatty acid and salt, that are more easily separated from glycerol. Then the process was continued with extraction by the solvent chloroform using the variable of test volume ratio (v/v) (1:1, 1:1.5, 1:2)  and the extraction time (20, 40, and 60 minutes). The results showed that the more volume of solvent used, gave less extraction time to produce high purity of glycerol. The highest purity produced in this study amounted to 90,9082% is obtained at the ratio of the volume solvent (v/v) 1:1 with extraction time 60 minutes.

THE USE OF GLYCEROL FOR THE CRYOPRESERVATION OF INCONNU’S SPERM

2017 ◽  
pp. 117-121
Author(s):  
Amina Kumarovna Karamuldaeva ◽  
Andrey Mikhailovich Tikhomirov
Keyword(s):  
Semen Quality ◽  
Life Time ◽  
Egg Yolk ◽  
Maximum Activity ◽  
Equilibration Time ◽  
Glycerol Concentration ◽  
Scientific Center ◽  
Spawning Period ◽  
Base Solution ◽  
Academy Of Sciences

The article studies the possibility to use glycerol as cryoprotectant, instead of dimethylsulfoxide for cryopreservation of sperm of inconnu ( Stenodus leucichthys Gueldenstaedtii, 1772). Investigations were carried out from 2015 to 2016 in the laboratory of the Southern Scientific Center, Russian Academy of Sciences, on the basis of the Astrakhan State Technical University. The material collected on the Alexander sturgeon hatcheries (the Astrakhan region) in the spawning period. Native sperm of 6 male inconnu species was used as a control means. The semen quality was determined in terms of moving activity (life time) of sperm after its activation by water. As the cryoprotectant there were used: base solution - 80%, sucrose - 1.71 g/l, mannite - 0.98 g/l, yolk - 10%, dimethylsulfoxide - 10% and base solution - 87%, sucrose - 1.71% g/l, mannite - 0.98 g/l, yolk - 10%, glycerol - 3 variants: 3; 5 and 10%. In order to provide the most complete penetration of cryoprotectants into the cells there were used electrostimulation of cell membranes. Equilibration time was 5 and 15 minutes. Thawing semen was performed in a water bath at a temperature of 38-40°C. For removing protectors from cells there was chosen a saline solution (0.7% NaCl) as isotonic solution. In tests using dimethylsulfoxide life activity of sex cells was 2 times lower than in tests with glycerol: 78 and 186.2 s at the end of equilibration and 52.3 and 128.9 s after thawing. Sperm showed maximum activity under 5% glycerol concentration during equilibration - 15 min. Concentration of 3% was insufficient, concentration of 10% was excessive, as it suppressed activity of sperm. Egg yolk which coagulated together with glycerol, making difficulty for observing, had to be excluded from the composition of cryoprotectant.

Viability of Edwardsiella tarda and Esherichia coli preserved with glycerol-tryptone soy broth (TSB) kept at freezing temperature

2013 ◽  
Vol 1 (2) ◽  
pp. 154
Author(s):  
Abdur Rohman ◽  
Frans Ijong ◽  
I K Suwetja
Keyword(s):  
Research And Development ◽  
Germ Plasm ◽  
Freezing Temperature ◽  
Experimental Methods ◽  
Edwardsiella Tarda ◽  
Diagnostic Tools ◽  
Glycerol Concentration ◽  
E Coli ◽  
Esherichia Coli ◽  
Escherchia Coli

Preservation of bacteria carried out in relation to the collection and preservation of germ plasm microbe is useful for research and development or for the establishment of diagnostic tools. Glycerol is a good preservation media but it is not known what doses should be used for effective preservation.  This research used two experimental  methods consisting of 2 factors and 3 treatments. This study aimed to find the best glycerol concentration that can be used to preserve Edwarsiella tarda and Escherchia coli in the -20ºC environment, to understand the viability of bacteria after being preserved and to describe the characteristics of the preserved bacteria. Treatments applied were 10%, 15% and 20%  glycerol in TSB. Viability of the bacteria was analyzed after 7, 14, 28, 35, and 42 days of preservation. Results showed that E.coli bacteria preserved in 15%  glycerol had the highest viability, i.e. 84% and preserved in 10% glycerol had the lowest viability, i.e. 80%. But for E. tarda bacteria preserved in 10% glycerol had the highest viability, i.e. 1.83% and preseved in 15% glycerol had the lowest viability, i.e. 0,55%. Preservasi bakteri dilakukan dalam kaitannya dengan koleksi dan konservasi plasma nutfah mikroba yang berguna untuk penelitian dan pengembangan atau untuk pembentukan alat diagnosa. Gliserol merupakan bahan preservasi yang baik, tetapi belum diketahui dosis yang baik dan efektif untuk preservasi bakteri Edwarsiella tarda dan Escherchia coli pada suhu -20ºC. Penelitian ini dilakukan dengan metode eksperimen yang terdiri dari 2 faktor dan 3 taraf perlakuan, masing-masing perlakuan dengan 3 kali ulangan, media preservasi yang digunakan adalah TSB dan gliserol dengan konsentrasi 10%, 15% dan 20%. Parameter yang diukur adalah viabilitas dan kecocokan/penyimpangan karakteristik biokimia. Penelitian ini dilaksanakan di Laboratorium Balai Karantina Ikan Pengendalian Mutu dan Keamanan Hasil Perikanan Manado, dari bulan September sampai dengan November 2013. Tujuan Penelitian ini adalah untuk menentukan konsentrasi gliserol dalam TSB sebagai media preservasi yang efektif dan efisien pada bakteri  Edwarsiella tarda dan Escherchia coli yang dipreservasi dengan suhu -20ºC dan disimpan selama 42  hari. Hasil penelitian menunjukkan adanya penurunan laju pertumbuhan bakteri selama preservasi. Persentase viabilitas  bakteri E. coli yang tertinggi selama preservasi diperoleh dengan penggunaan gliserol konsentrasi 15% dengan jumlah 84% dan yang terendah adalah dengan penggunaan konsentrasi 10% yakni sebesar 80%, sedangkan untuk E. tarda persentase viabilitas  bakteri yang tertinggi selama preservasi diperoleh dengan penggunaan gliserol konsentrasi 10% dengan jumlah 1,83% dan yang terendah adalah dengan penggunaan konsentrasi 15% yakni sebesar 0,55%. Berdasarkan uji statistik analisis variasi (ANAVA) didapat hasil F hitung E. tarda dan E. coli yang lebih besar  dari FTabel dengan tingkat kepercayaan 95 %.

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