Nano-structured poly-lactic-co-glycolic acid polymer surface features increase cell functions

2003 ◽  
Author(s):  
D.C. Miller ◽  
A. Thapa ◽  
K.M. Haberstroh ◽  
T.J. Webster
Keyword(s):  
Glycolic Acid ◽  
Polymer Surface ◽  
Surface Features ◽  
Cell Functions

Effects of PLGA Nano Patterns on the Responses of Healthy Osteoblasts

2012 ◽  
Vol 1412 ◽  
Author(s):  
Yongchen Wang ◽  
Lijuan Zhang ◽  
Linlin Sun ◽  
Thomas J. Webster
Keyword(s):  
Glycolic Acid ◽  
Bone Cells ◽  
Bone Cancer ◽  
Bone Cell ◽  
The Self ◽  
Healthy Human ◽  
Surface Features ◽  
Flat Surfaces ◽  
Cell Functions ◽  
Osteoblast Adhesion

ABSTRACTPoly(lactic-co-glycolic acid) (PLGA) films with flat surfaces and with 27nm, 190nm, 300nm, 400nm, and 520nm surface features were synthesized using a template method with polydimethylsiloxane (PDMS) molds. The nano patterns were transferred from the self-assembled polystyrene beads to the PLGA films through PDMS molds. After synthesis, the nano patterns were confirmed by AFM height scans. In order to investigate the influence of the materials on bone cells, healthy human osteoblasts were cultured on the PLGA films. The 27nm PLGA surface showed the maximum osteoblast adhesion density and a significant increase compared with the other surface features. For these reasons, and since previous studies have highlighted that similar nanometer surface features on PLGA decreased functions of other cancer cells, this study suggests that PLGA with 27nm surface features should be further studied for bone cancer applications where healthy bone cell functions need to be promoted and cancerous bone cell functions inhibited.

FORMATION OF COMBINED SURFACE FEATURES OF PROTRUSION ARRAY AND WRINKLES ATOP SHAPE-MEMORY POLYMER

2009 ◽  
Vol 16 (06) ◽  
pp. 929-933 ◽  
Author(s):  
L. SUN ◽  
Y. ZHAO ◽  
W. M. HUANG ◽  
T. H. TONG
Keyword(s):  
Shape Memory ◽  
Shape Memory Polymer ◽  
Polymer Surface ◽  
Cost Effective ◽  
Gold Layer ◽  
Surface Features ◽  
Cost Effective Approach ◽  
Different Types ◽  
Sputter Deposited ◽  
Steel Balls

We demonstrate a simple and cost-effective approach to realize two combined surface features of different scales together, namely submillimeter-sized protrusion array and microwrinkles, atop a polystyrene shape-memory polymer. Two different types of protrusions, namely flat-top protrusion and crown-shaped protrusion, were studied. The array of protrusions was produced by the Indentation-Polishing-Heating (IPH) process. Compactly packed steel balls were used for making array of indents. A thin gold layer was sputter deposited atop the polymer surface right after polishing. After heating for shape recovery, array of protrusions with wrinkles on the top due to the buckling of gold layer was produced.

Osteogenic Cell Attachment to Degradable Polymers

1991 ◽  
Vol 252 ◽  
Author(s):  
Kevin E. Healy ◽  
Davis Tsai ◽  
Jung E. Kim
Keyword(s):  
Cell Adhesion ◽  
Synthetic Peptide ◽  
Glycolic Acid ◽  
Cell Attachment ◽  
Polymer Surface ◽  
Degradable Polymers ◽  
Osteogenic Cell ◽  
Treatment Groups ◽  
Osteogenic Cells ◽  
Degradable Polyesters

ABSTRACTModifications were made to increase osteogenic cell adhesion to homo and copolymers of lactic and glycolic acid. A synthetic peptide containing the cell attachment signal Arginine-Glycine-Aspartate (RGD) was loaded into the polymers or adsorbed to the polymers' surfaces. Cell attachment was assayed after 24 hours incubation with an osteogenic cell line (ROS 17/2.8). Statistically significant differences in cell adhesion occurred between the polymers with the adsorbed peptides and the other treatment groups. Significant differences were not observed for the peptide loaded polymers and controls. These data indicate that precoating the polymer surface with a RGD-containing peptide prior to exposure to osteogenic cells increased cell attachment. For the current materials tested, the surface modification is preferred to increase osteogenic cell adhesion to degradable polyesters.

Improved Cardiomyocyte Functions of Carbon Nanofiber Cardiac Patches

2012 ◽  
Vol 1417 ◽  
Author(s):  
David A. Stout ◽  
Emilia Raimondo ◽  
Thomas J. Webster
Keyword(s):  
Electrical Stimulation ◽  
Glycolic Acid ◽  
Troponin I ◽  
Carbon Nanofiber ◽  
Myocardial Tissue ◽  
Human Cardiomyocytes ◽  
Cell Functions ◽  
Stimulation System ◽  
Myocardial Tissue Engineering

ABSTRACTThe objective of the present in vitro research was to determine cardiomyocyte functions on poly-lactic-co-glycolic acid (50:50 (PLA:PGA); PLGA) with greater amounts of carbon nanofibers (CNFs) using an in vitro electrical stimulation system for myocardial tissue engineering applications. The addition of CNFs can increase the conductivity and strength of pure PLGA. For this reason, different PLGA: CNF ratios (100:0, 75:25, 50:50, 25:75, 0:100 wt%) were created where conductivity and cytocompatibility properties under electrical stimulation with human cardiomyocytes were determined. Results showed that PLGA:CNF materials were conductive and that conductivity increased with greater amounts of PLGA added, from 0 S.m-1 for 100:0 wt% (pure PLGA) to 6.5x10-3 S.m-1 for 0:100 wt% (pure CNFs) materials. Furthermore, results indicated that cardiomyocyte cell density increased with continuous electrical stimulation (rectangular, 2 nm, 5 V/cm, 1 Hz) after 1, 3, and 5 days as well as a slight increase in Troponin I excretion compared to non-electrically stimulated normal cardiomyocyte cell functions. This study, thus, provides an alternative conductive scaffold using nanotechnology which should be further explored for numerous cardiovascular applications.

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