Using phylogenetic footprinting to discover putative Cis-regulatory elements and analysing their coexistence and related gene ontology

2006 ◽  
Author(s):  
Wei Shi ◽  
Wanlei Zhou
Keyword(s):  
Gene Ontology ◽  
Phylogenetic Footprinting ◽  
Regulatory Elements ◽  
Related Gene

Identifying cis-regulatory elements by statistical analysis and phylogenetic footprinting and analyzing their coexistence and related gene ontology

2007 ◽  
Vol 31 (3) ◽  
pp. 374-384 ◽  
Author(s):  
Wei Shi ◽  
Wanlei Zhou ◽  
Dakang Xu
Keyword(s):  
Gene Ontology ◽  
Transcription Factors ◽  
Related Species ◽  
Statistical Significance ◽  
Phylogenetic Footprinting ◽  
Regulatory Elements ◽  
Gene Promoters ◽  
Computational Molecular Biology ◽  
Novel Approach ◽  
Regulation Mechanisms

Discovery of cis-regulatory elements in gene promoters is a highly challenging research issue in computational molecular biology. This paper presents a novel approach to searching putative cis-regulatory elements in human promoters by first finding 8-mer sequences of high statistical significance from gene promoters of humans, mice, and Drosophila melanogaster, respectively, and then identifying the most conserved ones across the three species (phylogenetic footprinting). In this study, a conservation analysis on both closely related species (humans and mice) and distantly related species (humans/mice and Drosophila) is conducted not only to examine more candidates but also to improve the prediction accuracy. We have found 124 putative cis-regulatory elements and grouped these into 20 clusters. The investigation on the coexistence of these clusters in human gene promoters reveals that SP1, EGR, and NRF-1 are the dominant clusters appearing in the combinatorial combination of up to five clusters. Gene Ontology (GO) analysis also shows that many GO categories of transcription factors binding to these cis-regulatory elements match the GO categories of genes whose promoters contain these elements. Compared with previous research, the contribution of this study lies not only in the finding of new cis-regulatory elements, but also in its pioneering exploration on the coexistence of discovered elements and the GO relationship between transcription factors and regulated genes. This exploration verifies the putative cis-regulatory elements that have been found from this study and also gives new insight on the regulation mechanisms of gene expression.

Phylogenetic footprinting reveals multiple regulatory elements involved in control of the meiotic recombination gene,REC102

Yeast ◽  
2002 ◽  
Vol 19 (2) ◽  
pp. 99-114 ◽  
Author(s):  
Kai Jiao ◽  
John J. Nau ◽  
Marc Cool ◽  
William M. Gray ◽  
Jan S. Fassler ◽  
...  
Keyword(s):  
Meiotic Recombination ◽  
Phylogenetic Footprinting ◽  
Regulatory Elements ◽  
Recombination Gene
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